Agriculture, Life Sciences, and Veterinary Medicine
Botanical Microtechnique
Description
Permanent slides for microscopic study are indispensable in the teaching of a basic course in botany and also in specialized advanced courses. In some advanced courses, the students prepare many of the slides used in the course, but in elementary courses the slides are furnished. In the latter case, the slides either are purchased from commercial sources or made in the departmental laboratory.
Publication Details
Published: January 1, 1958
Publisher: Iowa State College Press
Location: Ames, IA
DOI: https://doi.org/10.31274/isudp.25
Downloads: 38742
Chapters
- 0. Botanical Microtechnique
- 1. Part 1 Introduction
- 2. Collecting and Subdividing Plant Materials
- 3. Killing, Fixing, and Storing Plant Tissues
- 4. Dehydration for Embedding
- 5. Infiltration and Embedding in Paraffin Wax
- 6. Microtome Sectioning of Material in Paraffin
- 7. Staining Paraffin Sections
- 8. The Celloidin Method
- 9. Sectioning Unembedded Tissues
- 10. The Preparation of Whole Mounts and Smears
- 11. Criteria of Successful Processing
- 12. Part 2 Introduction
- 13. Vegetative Organs of Vascular Plants
- 14. Thallophyta and Bryophyta
- 15. Reproductive Structures of Vascular Plants
- 16. Microscope Construction, Use, and Care
- 17. Photomicrography
- Index
- Bibliography
Citation
Sass J. 1958. Botanical Microtechnique