Animal Health

Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments

Authors
  • Stephen B. Gaul (Iowa State University)
  • Stephanie Wedel (Iowa State University)
  • Matthew M. Erdman (Iowa State University)
  • D.L. Hank Harris (Iowa State University)
  • Isabel Turney Harris (Iowa State University)
  • Kathleen E. Ferris (USDA National Veterinary Services Laboratories)
  • Lorraine J. Hoffman (Iowa State University)

Abstract

Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with Xba1. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using Xba1 restriction provides a screening method for identifying swine Salmonella serotypes.

Keywords: ASL R2176

How to Cite:

Gaul, S. B., Wedel, S., Erdman, M. M., Harris, D. H., Harris, I. T., Ferris, K. E. & Hoffman, L. J., (2007) “Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments”, Iowa State University Animal Industry Report 4(1). doi: https://doi.org/10.31274/ans_air-180814-809

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Published on
01 Jan 2007
Peer Reviewed