Animal Health

Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments

Authors: , , , , , ,

Abstract

Swine Salmonella isolates (n=674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with Xba1. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using Xba1 restriction provides a screening method for identifying swine Salmonella serotypes.

Keywords: ASL R2176

How to Cite: Gaul, S. B. , Wedel, S. , Erdman, M. M. , Harris, D. H. , Harris, I. T. , Ferris, K. E. & Hoffman, L. J. (2007) “Identification of Swine Salmonella serotypes Using Pulsed-field Gel Electrophoresis of Conserved Xba1 Fragments”, Iowa State University Animal Industry Report. 4(1). doi: https://doi.org/10.31274/ans_air-180814-809