Breeding Physiology

Cloning of the complete gene for pig PIT-1 and analysis of PIT-1 protein function

Authors
  • T.-P. Yu (Iowa State University)
  • H. S. Sun (Iowa State University)
  • Max F. Rothschild (Iowa State University)
  • C. K. Tuggle (Iowa State University)

Abstract

We have cloned and sequenced the complete proteincoding region of the pig PIT-1 gene. The pig PIT-1 gene is highly similar to the PIT-1 gene in other species (human, rodent, cattle). We also found some alternative forms of the pig PIT-1 gene. We have analyzed the protein function of the pig PIT-1 genes and one of its alternative forms; D3PIT-1. The functional studies showed that the pig PIT-1 protein can function normally at growth hormone (GH) and prolactin (PRL), whereas the pig D3PIT-1 protein cannot. These results imply that the pig PIT-1 gene is important in pig growth through regulation of GH and PRL gene expression, whereas its alternative forms might play different roles on PIT-1 target genes.

Keywords: ASL R1492

How to Cite:

Yu, T., Sun, H. S., Rothschild, M. F. & Tuggle, C. K., (1998) “Cloning of the complete gene for pig PIT-1 and analysis of PIT-1 protein function”, Iowa State University Animal Industry Report 1(1).

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Published on
01 Jan 1998
Peer Reviewed