Swine

Variants in the CD163 and CD169 Genes Associated with Host Response to PRRS and PCV2b in Nursery Pigs

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Abstract

Recently, pigs with complete resistance to PRRS have been produced by editing the CD163 gene. In this study, we genotyped pigs from previously conducted porcine reproductive and respiratory syndrome (PRRS) vaccination and PRRS-porcine circovirus type 2b (PCV2b) co-infection studies for several single nucleotide polymorphisms (SNPs) in CD163 and other candidate genes. The main reason for genotyping these additional SNPs was because CD163 was not included on the commercial porcine SNP panel originally used to genotype these animals. The objective of this study was to identify natural variation in these genes associated with host response to PRRS virus (PRRSV) and PCV2b co-infection following prior vaccination, or not, with a PRRS modified live virus (MLV) vaccine. Several SNPs were significantly associated with PRRS and PCV2b viral load (VL), as well as average daily gain (ADG) following vaccination and following co-infection. The effects of some SNPs depended on previous vaccination for PRRS or genotype at the WUR10000125 (WUR) SNP (previously associated with host response to PRRS and coinfection with PRRSV and PCV2b). Interestingly, one SNP in CD163 and one SNP in CD169 causing amino acid substitutions had significant effects on PRRSV/PCV2b VL and/or ADG, regardless of whether/not pigs had been vaccinated for PRRS. The identified SNPs are potential genetic markers that can be used to select for increased natural resistance to PRRSV and PCV2b co-infection.

How to Cite:

Dekkers, J. C., Tuggle, C. K., Dong, Q., Lunney, J., Lim, K., Dunkelberger, J. & Rowland, B., (2019) “Variants in the CD163 and CD169 Genes Associated with Host Response to PRRS and PCV2b in Nursery Pigs”, Iowa State University Animal Industry Report 16(1).