Animal Health

Lawsonia intracellularis ELISA: A New Test at the ISU-VDL

Authors
  • Ronaldo L. Magtoto (Iowa State University)
  • John K. Johnson (Iowa State University)
  • Sheela Ramamoorthy (Iowa State University)

Abstract

Porcine Proliferative Enteropathy (PPE) is a common disease of swine which is caused by the intracellular bacterium, Lawsonia intracellularis.

The performance of a recently available commercial ELISA for L. intracellularis was evaluated by comparison with an immuno-peroxidase monolayer assay (IPMA) and found to have at least 97% correlation. The same assay when conducted at different laboratories showed 100% agreement in results. Examination of serum samples from various cases submitted to the Iowa State University Veterinary Diagnostic laboratory indicated that 87% of the herds examined were sero-positive for L. intracellularis. Therefore, the Enzyme-Linked Immunosorbent Assay (ELISA) for Lawsonia intracellularis is a useful and reliable test which allows veterinary practitioners and producers to obtain same day results on swine serum samples submitted.

Keywords: ASL R2486

How to Cite:

Magtoto, R. L., Johnson, J. K. & Ramamoorthy, S., (2010) “Lawsonia intracellularis ELISA: A New Test at the ISU-VDL”, Iowa State University Animal Industry Report 7(1). doi: https://doi.org/10.31274/ans_air-180814-645

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Published on
01 Jan 2010
Peer Reviewed