Avian

SIRT1 attenuates the host resistance to Salmonella by negatively regulating the immune response in chicken

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Abstract

Objective: Elucidate the role of SIRT1 in Salmonella infection, enrich our understanding of its role in innate immune regulation in chicken, and determine whether its modulation can provide a theoretical basis for defense against Salmonella in chicken.Method: The role of SIRT1 in Salmonella infection and its effects on immune factor levels were determined at the cellular level using flow cytometry, qPCR, ELISA, and other methods. Additionally, transcriptome sequencing and acetylome analysis were performed on SIRT1-inhibited cells infected with Salmonella, and relevant immune indicators were examined in chicks with inhibited SIRT1 after Salmonella infection.Results: Investigation of intracellular bacterial load and immune factor levels after SIRT1 inhibition or activation revealed that activation of SIRT1 facilitates Salmonella infection, while its inhibition leads to increased levels of immune factors. This is consistent with the traditional view that SIRT1 exerts anti-inflammatory effects in the immune response. Omics analysis further indicated that SIRT1 may negatively regulate the immune response by modulating immune-related genes and pathways. Finally, in vivo experiments showed that SIRT1 inhibition enhance resistance to Salmonella infection in chicken by resulting in a significant increase in pro-inflammatory factors.Conclusion: The present results show that SIRT1 promotes Salmonella infection of chicken macrophages and reduces host defense by negatively regulating NF-κB activation and the production of pro-inflammatory cytokines. This finding enriches our understanding of the function of SIRT1 in Salmonella infection and lays a theoretical foundation for defense against Salmonella in chicken.

Keywords: 2026

How to Cite:

Gao, q. & Li, Q., (2026) “SIRT1 attenuates the host resistance to Salmonella by negatively regulating the immune response in chicken”, World Congress on Genetics Applied to Livestock Production Digital Archive 2026(1): 2267965. doi: https://doi.org/10.31274/wcgalp.24546

Rights: 1