Characterization of Campylobacter coli isolated from pig, sheep, poultry, wild bird, river and shellfish using MALDI-TOF and comparison of their protein spectra to identify relationships between sources
- A. Rincé (Université de Caen)
- B. Nagard (Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail (ANSES))
- E. Cauvin (Labéo)
- F. Benoit (Labéo)
- J. Serghine (Ifremer)
- M. Denis (Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail (ANSES))
- M. Gourmelon (Ifremer)
- M. Meunier (Labéo)
- V. Rose (Anses)
Abstract
The sanitary quality of shellfish-harvesting areas is a key issue in France, the leading shellfish producer in Europe. Campylobacter spp was detected in coastal catchments and shellfish-harvesting areas in Brittany and Normandy, France (Rincé et al., 2018). This pathogen is excreted by many animals whether wild birds or livestock (Mughini-Gras et al., 2016). These participate in the contamination of the environment directly or via manure spreading. Comparison of PFGE profiles or MLST types have proved their effectiveness in determining the origin of human cases of campylobacteriosis or surface water contamination (Denis et al., 2009; Denis et al., 2011a; Clark et al., 2011; Jonas et al., 2015; Mughini-Gras et al., 2016). Identify the sources of shellfish contamination with these techniques is possible but these latter are expensive and long to implement. An alternative may be the typing of strains by MALDI-TOF MS. Links of MALDI-types of Campylobacter to their MLST types were described (Zautner et al., 2013). Moreover, the MALDI-TOF MS technique is easy to perform and inexpensive.
How to Cite:
Rincé, A., Nagard, B., Cauvin, E., Benoit, F., Serghine, J., Denis, M., Gourmelon, M., Meunier, M. & Rose, V., (2019) “Characterization of Campylobacter coli isolated from pig, sheep, poultry, wild bird, river and shellfish using MALDI-TOF and comparison of their protein spectra to identify relationships between sources”, SafePork 13(1), 163-164. doi: https://doi.org//safepork.11410
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