<?xml version="1.0" encoding="UTF-8"?>
<article article-type="research-article" dtd-version="2.3" xml:lang="EN" annotation-version="1.0"><front><journal-meta><journal-id journal-id-type="publisher-id">mmb</journal-id><journal-id journal-id-type="hwp">mmb</journal-id><journal-title>Meat and Muscle Biology</journal-title><abbrev-journal-title abbrev-type="full">MMB</abbrev-journal-title><issn pub-type="ppub">XXXX-XXXX</issn><issn pub-type="epub">XXXX-XXXX</issn><publisher><publisher-name>American Meat Science Association</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">2017-01-0003</article-id><article-id pub-id-type="doi">10.22175/mmb2017.01.0003</article-id><article-categories><subj-group subj-group-type="heading"><subject/></subj-group></article-categories><title-group><article-title>Assessment of Postmortem Aging Effects on Texas-style Barbecue Beef Briskets</article-title></title-group><contrib-group><contrib contrib-type="author" contact-id="4179573151956947641" contact-type="auto"><name><surname>Harris</surname><given-names>McKensie K.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib><contrib contrib-type="author" contact-id="3475446493016519118" contact-type="auto"><name><surname>Riley</surname><given-names>Ray R.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib><contrib contrib-type="author" contact-id="14123818815514539121" contact-type="auto"><name><surname>Arnold</surname><given-names>Ashley N.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib><contrib contrib-type="author" contact-id="2893544581157141340" contact-type="auto"><name><surname>Miller</surname><given-names>Rhonda K.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib><contrib contrib-type="author" contact-id="15821914127467306215" contact-type="auto"><name><surname>Griffin</surname><given-names>Davey B.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib><contrib contrib-type="author" contact-id="16628871945035482657" contact-type="auto"><name><surname>Gehring</surname><given-names>Kerri B.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib><contrib contrib-type="author" corresp="yes" contact-id="0" contact-type="auto"><name><surname>Savell</surname><given-names>Jeffrey W.</given-names></name><aff><label>1</label>Department of Animal Science, Texas A&amp;M University, Texas A&amp;M AgriLife Research, College Station, TX 77843, USA</aff></contrib></contrib-group><author-notes><corresp id="cor1">*Corresponding author. Email: <email>j-savell@tamu.edu</email> (J. Savell)</corresp></author-notes><pub-date pub-type="ppub"><month>06</month><year>2017</year></pub-date><volume>1</volume><issue>1</issue><fpage>46</fpage><lpage>52</lpage><history><date date-type="received"><day>09</day><month>01</month><year>2017</year></date><date date-type="accepted"><day>30</day><month>03</month><year>2017</year></date></history><permissions><copyright-year>2017</copyright-year><copyright-holder>American Meat Science Association</copyright-holder><license license-type="open-access"><p>This is an open access article distributed under the CC BY-NC-ND license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-nd/4.0/" xmlns:xlink="http://www.w3.org/1999/xlink">http://creativecommons.org/licenses/by-nc-nd/4.0/</ext-link>)</p></license></permissions><abstract><p>Palatability traits (tenderness, juiciness, flavor, and overall liking) of beef briskets (<italic>n</italic> = 48) were evaluated for 3 different postmortem aging period comparisons: 7 d vs. 21 d (Set 1), 21 d vs. 35 d (Set 2), and 7 d vs. 35 d (Set 3). Briskets were prepared as Texas-style barbecue by seasoning with salt and pepper and smoking with oak wood over a long period (approx. 11 h) using a commercial oven designed for such purposes until deemed tender (approx. 85°C internal temperature). Within each treatment set, Warner-Bratzler shear (WBS) force measurements did not differ (<italic>P</italic> &gt; 0.05) between aging days; however, WBS force measurements for point (<italic>Mm. pectorales superficiales</italic> composed of the <italic>M. pectoralis transversus and M. pectoralis descendens</italic>) were lower (<italic>P</italic> &lt; 0.05) compared to the flat portions (<italic>M. pectorals profundus</italic>) within each aging set. Consumer panelists did not (<italic>P</italic> &gt; 0.05) detect differences between aging days within each set for overall liking, flavor liking, tenderness liking, and juiciness liking. Conversely, differences were found between brisket point and flat portions; Set 1 differed for flavor liking (flat &gt; point; <italic>P</italic> = 0.0348) and juiciness liking (point &gt; flat; <italic>P</italic> = 0.0004), Set 2 differed for overall liking (flat &gt; point; <italic>P</italic> = 0.0499) and juiciness liking (point &gt; flat; <italic>P</italic> &lt; 0.0001), and Set 3 differed for overall liking (flat &gt; point; <italic>P</italic> = 0.0296) and juiciness liking (point &gt; flat; <italic>P</italic> = 0.0112). Our findings indicate postmortem aging did not improve beef brisket palatability, but we did note differences between point and flat portions. Preparing beef briskets as Texas-style barbecue resulted in products with low WBS values and high consumer palatability ratings, which helps demonstrate why barbecued briskets are so popular.</p></abstract><kwd-group><title>Keywords</title><kwd>aging</kwd><kwd>beef</kwd><kwd>brisket</kwd><kwd>consumer panels</kwd><kwd>warner-bratzler shear force</kwd></kwd-group></article-meta><custom-meta-wrap><custom-meta><meta-name>author</meta-name><meta-value>Harris McKensie K.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Riley Ray R.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Arnold Ashley N.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Miller Rhonda K.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Griffin Davey B.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Gehring Kerri B.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Savell Jeffrey W.</meta-value></custom-meta></custom-meta-wrap><ar:concepts xmlns:ar="http://appliedrelevance.com"><ar:concept><ar:id>fa6a5a3224d7da66d9e0bdec25f62cf0</ar:id><ar:name>Testing</ar:name><ar:path a="a">Agronomy|Training &amp; Education|Testing</ar:path><ar:taxonomy>Agronomy</ar:taxonomy></ar:concept><ar:concept><ar:id>12d60d1447d4e3a8e677e155bc785d44</ar:id><ar:name>Nutrient</ar:name><ar:path a="a">Agronomy|Ecology &amp; Environment|Fate and Transport Processes|Nutrient</ar:path><ar:taxonomy>Agronomy</ar:taxonomy></ar:concept><ar:concept><ar:id>58412bbc1e3f44b73e9be60f47ed3857</ar:id><ar:name>thicknesses</ar:name><ar:path a="a">Soils|Miscellaneous|thicknesses</ar:path><ar:taxonomy>Soils</ar:taxonomy></ar:concept></ar:concepts></front><body><sec sec-type="introduction"><title>Introduction</title><p>The demand for beef brisket has increased due to its rising popularity among Texas Barbecue enthusiasts (<xref ref-type="bibr" rid="bib5">Franklin and Mackay, 2015</xref>, <xref ref-type="bibr" rid="bib6">Goldwyn and Blonder, 2016</xref>, <xref ref-type="bibr" rid="bib17">Walsh, 2016</xref>). Beyond barbecue, this beef commodity is becoming more mainstream in restaurants where chefs are refining the use of brisket for specialty ground beef items. Along with these uses, quick service restaurants and retailers, such as Arby’s and H-E-B, have empowered the rise in brisket consumption by offering specialty sandwiches and products that go beyond the traditional hamburger. Therefore, it is important for the beef industry to explore options for adding value to brisket by better understanding and/or enhancing its flavor, juiciness, and tenderness; all important aspects for pit masters, chefs, retailers, and consumers.</p><p>A Canadian study evaluated the palatability characteristics of 33 beef muscles and found that the overall tenderness ratings for point (<italic>Mm. pectorales superficiales: M. pectoralis transversus and M. pectoralis descendens</italic> or more simply known as the superficial pectoral) and flat (<italic>M. pectoralis profundus</italic> or deep pectoral) portions that make up the brisket, were ranked by a trained sensory panel as 2 of the least tender of the muscles studied (<xref ref-type="bibr" rid="bib7">Jeremiah et al., 2003</xref>). In addition, there have been multiple studies that have evaluated tenderness of beef brisket by Warner-Bratzler Shear (WBS) force (<xref ref-type="bibr" rid="bib1">Belew et al., 2003</xref>, <xref ref-type="bibr" rid="bib8">Johnson et al., 1988</xref>, <xref ref-type="bibr" rid="bib11">Ramsbottom et al., 1945</xref>). In one of the earliest studies characterizing muscles in the beef carcass, <xref ref-type="bibr" rid="bib11">Ramsbottom et al. (1945)</xref> ranked muscles from most to least tender, with the deep and superficial pectoral muscles falling in the bottom 25% for shear force and organoleptic ratings. This same study identified the deep pectoral as being more tender than the superficial pectoral, whereas <xref ref-type="bibr" rid="bib8">Johnson et al. (1988)</xref> found the superficial pectoral to be more tender than the deep pectoral. A more recent study conducted by <xref ref-type="bibr" rid="bib1">Belew et al. (2003)</xref> utilized the tenderness threshold identified by <xref ref-type="bibr" rid="bib12">Shackelford et al. (1991)</xref> to classify various muscles as “very tough,” “tough,” “intermediate,” “tender,” and “very tender” based on WBS force measurements. The superficial pectoral was classified as “tender,” whereas the deep pectoral was categorized as “tough” (<xref ref-type="bibr" rid="bib1">Belew et al., 2003</xref>). Previous studies examining the muscles comprising the brisket have reported conflicting results, showing great variability in WBS force values and tenderness rankings.</p><p>Not only has historical research classified muscles based on palatability attributes, but it has also identified useful ways in improving the eating experience of beef. Research has shown that tenderness is 1 of the 3 most important factors in beef eating quality (<xref ref-type="bibr" rid="bib9">Legako et al., 2016</xref>), and can be improved through postmortem aging (<xref ref-type="bibr" rid="bib2">Calkins and Seideman, 1988</xref>, <xref ref-type="bibr" rid="bib3">Doty and Pierce, 1961</xref>, <xref ref-type="bibr" rid="bib4">Eilers et al., 1996</xref>, <xref ref-type="bibr" rid="bib13">Smith et al., 1978</xref>). Although most researchers have conducted studies on tenderness for “middle meats” and muscles from the round, there has been limited work evaluating the postmortem aging effect on other muscle groups such as those comprising the brisket. <xref ref-type="bibr" rid="bib13">Smith et al. (1978)</xref> found the deep and superficial pectoral muscles to achieve maximum postmortem tenderization after 5 d and 28 d, respectively. Thus, there is a possibility of eliciting changes in palatability characteristics that would add value to the previously considered “tough” brisket.</p><p>Cookery method is one of multiple factors that can impact the tenderness of beef. <xref ref-type="bibr" rid="bib13">Smith et al. (1978)</xref> prepared beef samples by roasting to an internal temperature of 75°C in an electric oven. <xref ref-type="bibr" rid="bib1">Belew et al. (2003)</xref> cooked brisket steaks to 70°C on a flat-top grill. Thus, data from previous studies should be evaluated with cooking difference in mind. Palatability of traditional Texas-style smoked briskets that are cooked at low temperatures (93.3°C to 121.1°C) for extended lengths of time (8 to 12 h) (<xref ref-type="bibr" rid="bib5">Franklin and Mackay, 2015</xref>, <xref ref-type="bibr" rid="bib6">Goldwyn and Blonder, 2016</xref>, <xref ref-type="bibr" rid="bib17">Walsh, 2016</xref>) has not been evaluated. The current study was designed to evaluate the effect of postmortem aging periods on Texas-style smoked briskets. Determination of postmortem aging effects on the palatability of briskets could provide the opportunity to add value and promote more effective merchandising of beef briskets.</p></sec><sec sec-type="materials|methods"><title>Materials and Methods</title><p>Consumer panel procedures were approved by the Texas A&amp;M Institutional Review Board (IRB2015–0498M).</p><sec><title>Product collection</title><p>Twenty-four A-maturity, yield grade 1 to 4 beef carcasses with small, modest, or moderate marbling (<xref ref-type="bibr" rid="bib15">USDA, 2016a</xref>) and carcass weights ranging from 320.0 kg to 438.4 kg were selected 48 h postmortem at a commercial harvest and processing facility for use in this study. Carcasses were selected by trained individuals who estimated the amount of intramuscular fat (marbling) at the 12th and 13th rib interface along with lean color and skeletal ossification (<xref ref-type="bibr" rid="bib15">USDA, 2016a</xref>). Paired, untrimmed beef briskets, deckle-on, boneless (IMPS 119), as described by <xref ref-type="bibr" rid="bib14">USDA (2010)</xref>, were removed from each of the selected carcasses. Briskets (<italic>n</italic> = 48) then were vacuum packaged, boxed, stored under refrigerated conditions, and transported to the Texas A&amp;M Rosenthal Meat Science and Technology Center (College Station, TX).</p></sec><sec><title>Treatment assignment and storage</title><p>Upon receipt, each pair of briskets was assigned to 1 of 3 aging period comparison sets: 7 versus 21 d (Set 1); 21 versus 35 d (Set 2); and 7 versus 35 d (Set 3). Briskets from 1 carcass side were assigned to a consistent age day treatment within each set. The pack date was identified as Day 0 for all aging periods. Briskets were aged under refrigerated conditions (2°C to 4°C) for each designated treatment length. After each aging period, briskets were frozen (–40°C) and stored (–10°C) for a minimum of 5 d.</p></sec><sec><title>Raw product preparation</title><p>Briskets were thawed (2°C to 4°C) for 5 to 6 d. Thawed briskets were unpackaged, deckle fat was removed, and sternum and external fat were trimmed to 0.64 cm to create a deckle-off, boneless brisket (IMPS 120) as described by <xref ref-type="bibr" rid="bib14">USDA (2010)</xref>. Trimmed briskets were weighed (<xref ref-type="table" rid="tbl1">Table 1</xref>), and a seasoning mix consisting of 89 g Morton’s Kosher Salt (Grand Saline, TX) and 42 g 16-mesh coarse ground black pepper (REO Spice and Seasoning, Huntsville, TX) was applied to all surfaces of each brisket. Briskets were held in insulated containers, transported (∼132 km) to Southside Market and Barbeque (Elgin, TX), and cooked using typical commercial methods.</p><table-wrap id="tbl1"><label>Table 1.</label><caption><p>Means and standard deviations for weights, cook yield, temperatures, and cook duration of briskets</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Parameter</td><td align="center"><italic>n</italic><sup>1</sup></td><td align="center">Mean</td><td align="center">SD</td></tr></thead><tbody><tr><td>Raw weight (kg)</td><td align="char" char=".">48</td><td align="char" char=".">5.60</td><td align="char" char=".">0.75</td></tr><tr><td>Cooked weight (kg)</td><td align="char" char=".">48</td><td align="char" char=".">3.51</td><td align="char" char=".">0.51</td></tr><tr><td>Cook yield (%)</td><td align="char" char=".">48</td><td align="char" char=".">62.60</td><td align="char" char=".">2.01</td></tr><tr><td>Initial raw temperature (°C)</td><td align="char" char=".">48</td><td align="char" char=".">3.03</td><td align="char" char=".">0.73</td></tr><tr><td>Final cooked temperature (°C)</td><td align="char" char=".">48</td><td align="char" char=".">85.42</td><td align="char" char=".">2.28</td></tr><tr><td>Cook duration (h)</td><td align="char" char=".">39</td><td align="char" char=".">11.00</td><td align="char" char=".">0.58</td></tr></tbody></table><table-wrap-foot><fn><p><sup>1</sup>Number of briskets evaluated.</p></fn></table-wrap-foot></table-wrap></sec><sec><title>Cooking</title><p>After initial internal raw brisket temperatures were recorded, briskets were spaced evenly on one of six racks in an Oyler Barbecue Pit (J&amp;R Manufacturing, Inc., Mesquite, TX) and smoked using oak wood at a pit temperature of 98.8°C for approximately 11 h. As brisket internal temperatures approached the doneness threshold (approx. 85°C internal temperature), an experienced pit master assessed each brisket manually for pliability as an indicator that the desired level of tenderness had been achieved. Final internal temperatures were recorded before finished briskets were removed from the pit, weighed, wrapped in peach treated butcher paper (Norpak, Newark, NJ) and stored in an insulated container for transport to the Texas A&amp;M University sensory facilities (College Station). Brisket weights and cooking data are reported in <xref ref-type="table" rid="tbl1">Table 1</xref>.</p></sec><sec><title>Sensory evaluation</title><p>Cooked briskets were held in the insulated containers for approximately 2 h, which included transportation and staging before the sensory panels. Ten minutes before serving, briskets were taken out of the insulated containers and the peach paper wrapping was removed. A knife was used to separate the point from flat portion at the most posterior edge of the hard sternum fat. Each portion was rewrapped in a new sheet of peach paper and held in an oven (Alto-Shaam, Milwaukee, WI) set at 93.3°C until subsequent slicing and serving.</p><p>Slicing varied for each portion. The point portion was divided in half with a knife cut starting at the apex of the brisket (cranial end) and continuing to the cut face (perpendicular to the point/flat separation). The half furthest from the hard sternum fat (craniodorsal half) was used to remove a 2.54 cm thick slice (from the cut surface) for WBS force, followed by six subsequent center cut slices, each 1.27 cm thick and 12.7 cm wide. Slices from the flat portion were removed according to the following: the first slice (2.54 cm thick), destined for WBS force, was removed at the interface of point/flat separation; 6 subsequent center cut slices 1.27 cm thick and 12.7 cm wide (accomplished by removing 2 equidistant ends to make a center cut slice) were separated. Prepared slices from each portion destined for consumer evaluation were placed on individually labeled, clear serving plates and served to consumer panelists. A plastic knife and fork were provided with each sample to assist in sample tasting.</p><p>The panel was conducted at the Kleberg Animal and Food Sciences Center at Texas A&amp;M University (College Station). Panelists (<italic>n</italic> = 83) were recruited from the Bryan/College Station area via electronic survey. Compensation in the amount of $25 USD was awarded to all panelists who completed the study. Before beginning each session, panelists were given verbal instructions and asked to complete a consent form, demographics survey (<xref ref-type="table" rid="tbl2">Table 2</xref>), and a meat consumption questionnaire (<xref ref-type="table" rid="tbl3">Table 3</xref>). Panelists then were seated in individual testing booths equipped with red theater gel lights. Samples were served warm, in a random order, and identified with random 3-digit codes. Nabisco Unsalted Tops Premium Saltine Crackers (Kraft Foods Global, Inc., East Hanover, NJ) and double distilled, deionized water were provided to panelists to cleanse their palate between samples. Panelists were asked to evaluate brisket slice attributes based on a 9-point scale. Attributes included: overall liking (1 = dislike extremely; 9 = like extremely), flavor liking (1 = dislike extremely; 9 = like extremely), juiciness liking (1 = dislike extremely; 9 = like extremely), and tenderness liking (1 = dislike extremely; 9 = like extremely).</p><table-wrap id="tbl2"><label>Table 2.</label><caption><p>Demographic summary of consumer panelists (<italic>n</italic> = 83)</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Demographic</td><td align="center"><italic>n</italic><sup>1</sup></td><td align="center">%</td></tr></thead><tbody><tr><td colspan="3">Sex</td></tr><tr><td> Male</td><td align="char" char=".">40</td><td align="char" char=".">48</td></tr><tr><td> Female</td><td align="char" char=".">43</td><td align="char" char=".">52</td></tr><tr><td colspan="3">Age</td></tr><tr><td> 20 yr or younger</td><td align="char" char=".">5</td><td align="char" char=".">6</td></tr><tr><td> 21 to 25 yr</td><td align="char" char=".">23</td><td align="char" char=".">28</td></tr><tr><td> 26 to 35 yr</td><td align="char" char=".">18</td><td align="char" char=".">22</td></tr><tr><td> 36 to 45 yr</td><td align="char" char=".">7</td><td align="char" char=".">8</td></tr><tr><td> 46 to 55 yr</td><td align="char" char=".">11</td><td align="char" char=".">13</td></tr><tr><td> 56 to 65 yr</td><td align="char" char=".">9</td><td align="char" char=".">11</td></tr><tr><td> 66 yr and older</td><td align="char" char=".">10</td><td align="char" char=".">12</td></tr><tr><td colspan="3">Working status</td></tr><tr><td> Not employed</td><td align="char" char=".">11</td><td align="char" char=".">12</td></tr><tr><td> Part-time</td><td align="char" char=".">12</td><td align="char" char=".">13</td></tr><tr><td> Full-time</td><td align="char" char=".">33</td><td align="char" char=".">37</td></tr><tr><td> Student</td><td align="char" char=".">33</td><td align="char" char=".">37</td></tr><tr><td colspan="3">Annual household income</td></tr><tr><td> Below $25,000</td><td align="char" char=".">17</td><td align="char" char=".">20</td></tr><tr><td> $25,001 to 49,999</td><td align="char" char=".">14</td><td align="char" char=".">17</td></tr><tr><td> $50,000 to 74,999</td><td align="char" char=".">17</td><td align="char" char=".">20</td></tr><tr><td> $75,000 to 99,999</td><td align="char" char=".">13</td><td align="char" char=".">16</td></tr><tr><td> $100,000 or more</td><td align="char" char=".">22</td><td align="char" char=".">27</td></tr><tr><td colspan="3">Ethic background</td></tr><tr><td> White</td><td align="char" char=".">71</td><td align="char" char=".">86</td></tr><tr><td> Hispanic</td><td align="char" char=".">10</td><td align="char" char=".">12</td></tr><tr><td> Asian or Pacific Islander</td><td align="char" char=".">1</td><td align="char" char=".">1</td></tr><tr><td> Black</td><td align="char" char=".">1</td><td align="char" char=".">1</td></tr><tr><td> American Indian</td><td align="char" char=".">0</td><td align="char" char=".">0</td></tr><tr><td> Other</td><td align="char" char=".">0</td><td align="char" char=".">0</td></tr></tbody></table><table-wrap-foot><fn><p><sup>1</sup>Number of responses.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tbl3"><label>Table 3.</label><caption><p>Meat consumption profile of consumer panelists (<italic>n =</italic> 83)</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Meat consumption pattern</td><td align="center"><italic>n</italic><sup>1</sup></td><td align="center">%</td></tr></thead><tbody><tr><td colspan="3">Meat consumption</td></tr><tr><td> Yes</td><td align="char" char=".">82</td><td align="char" char=".">99</td></tr><tr><td> No</td><td align="char" char=".">1</td><td align="char" char=".">1</td></tr><tr><td colspan="3">Type of meat consumption</td></tr><tr><td> Beef</td><td align="char" char=".">83</td><td align="char" char=".">100</td></tr><tr><td> Pork</td><td align="char" char=".">82</td><td align="char" char=".">99</td></tr><tr><td> Chicken</td><td align="char" char=".">83</td><td align="char" char=".">100</td></tr><tr><td> Fish</td><td align="char" char=".">77</td><td align="char" char=".">93</td></tr><tr><td colspan="3">Frequency of beef consumption</td></tr><tr><td> Daily</td><td align="char" char=".">7</td><td align="char" char=".">8</td></tr><tr><td> 5 or more times per wk</td><td align="char" char=".">18</td><td align="char" char=".">22</td></tr><tr><td> 3 or more times per wk</td><td align="char" char=".">40</td><td align="char" char=".">48</td></tr><tr><td> Once per wk</td><td align="char" char=".">16</td><td align="char" char=".">19</td></tr><tr><td> Once every 2 wk</td><td align="char" char=".">1</td><td align="char" char=".">1</td></tr><tr><td> Less than once every 2 wk</td><td align="char" char=".">1</td><td align="char" char=".">1</td></tr><tr><td colspan="3">Frequency of beef consumption</td></tr><tr><td colspan="3">At home</td></tr><tr><td> None</td><td align="char" char=".">2</td><td align="char" char=".">3</td></tr><tr><td> Once weekly</td><td align="char" char=".">20</td><td align="char" char=".">25</td></tr><tr><td> Twice weekly</td><td align="char" char=".">26</td><td align="char" char=".">33</td></tr><tr><td> 3 times weekly</td><td align="char" char=".">18</td><td align="char" char=".">23</td></tr><tr><td> 4 times weekly</td><td align="char" char=".">7</td><td align="char" char=".">9</td></tr><tr><td> 5 or more times weekly</td><td align="char" char=".">7</td><td align="char" char=".">9</td></tr><tr><td colspan="3">At a restaurant</td></tr><tr><td> None</td><td align="char" char=".">3</td><td align="char" char=".">4</td></tr><tr><td> Once weekly</td><td align="char" char=".">40</td><td align="char" char=".">49</td></tr><tr><td> Twice weekly</td><td align="char" char=".">20</td><td align="char" char=".">25</td></tr><tr><td> 3 times weekly</td><td align="char" char=".">7</td><td align="char" char=".">9</td></tr><tr><td> 4 times weekly</td><td align="char" char=".">7</td><td align="char" char=".">9</td></tr><tr><td> 5 or more times weekly</td><td align="char" char=".">4</td><td align="char" char=".">5</td></tr><tr><td colspan="3">Preferred degree of doneness</td></tr><tr><td> Rare</td><td align="char" char=".">3</td><td align="char" char=".">4</td></tr><tr><td> Medium rare</td><td align="char" char=".">21</td><td align="char" char=".">25</td></tr><tr><td> Medium</td><td align="char" char=".">6</td><td align="char" char=".">7</td></tr><tr><td> Medium well</td><td align="char" char=".">38</td><td align="char" char=".">45</td></tr><tr><td> Well done</td><td align="char" char=".">17</td><td align="char" char=".">20</td></tr><tr><td colspan="3">Type of beef purchased</td></tr><tr><td> Grass-fed</td><td align="char" char=".">11</td><td align="char" char=".">12</td></tr><tr><td> Traditional</td><td align="char" char=".">71</td><td align="char" char=".">75</td></tr><tr><td> Aged</td><td align="char" char=".">6</td><td align="char" char=".">6</td></tr><tr><td> Organic</td><td align="char" char=".">7</td><td align="char" char=".">7</td></tr></tbody></table><table-wrap-foot><fn><p><sup>1</sup>Number of responses.</p></fn></table-wrap-foot></table-wrap></sec><sec><title>Warner Bratzler shear force</title><p>Single slices taken from the point and flat portions of each cooked brisket were placed in a single layer on a plastic tray, covered with plastic wrap and stored (2°C to 4°C) for 12 h. Slices were equilibrated to room temperature before being trimmed of bark (exterior crust formed during cooking), visible fat, and heavy connective tissue to expose muscle fiber orientation. Six cores, 1.27 cm in diameter, were removed parallel to the muscle fiber for testing. Each core was sheared once perpendicular to the muscle fiber on a United Testing machine (United SSTM-500, Huntington Beach, CA) at a cross-head speed of 200 mm/min using a 10.0 kg load cell and a 1.02-cm-thick V-shape blade with a 60° angle and a half-round peak. The peak force needed to shear each core was recorded, and the average of the six cores was used for analysis.</p></sec><sec><title>Statistical analysis</title><p>Data were analyzed using the PROC GLM function of SAS (v9.3; SAS Inst. Inc., Cary, NC) with ɑ &lt; 0.05. Data were analyzed so that only aging treatments within carcass were evaluated and carcass was included as a fixed effect. Therefore, 3 analyses were conducted that compared 7 d versus 21 d aging, 21 d versus 35 d aging, and 7 d versus 35 d aging. Main effects included in the model were aging time, portion (flat versus point) and their interaction. Least squares means were calculated and were separated using the PDIFF option (<italic>P</italic> &lt; 0.05) where appropriate.</p></sec></sec><sec sec-type="results|discussion"><title>Results and Discussion</title><sec><title>Warner-Bratzler shear force</title><p>There were no interactions (<italic>P</italic> &gt; 0.05) between aging treatment and portion for WBS values (data not reported in tabular form). Least squares means for WBS force values for Set 1, 2, and 3 main effects are reported in <xref ref-type="table" rid="tbl4">Table 4</xref>. Though briskets aged for a greater number of days were not more tender (<italic>P</italic> &gt; 0.05) than those aged for fewer days, all WBS force values fell well within the “very tender” threshold (&lt; 31.38 N) as defined by <xref ref-type="bibr" rid="bib1">Belew et al. (2003)</xref>.</p><table-wrap id="tbl4"><label>Table 4.</label><caption><p>Least squares means and SE for WBS force values obtained from each set comparison for aging treatment and brisket portion</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Aging treatment and brisket portion comparison</td><td align="center"><italic>n</italic><sup>1</sup></td><td align="center">WBS force (N)</td></tr></thead><tbody><tr><td colspan="3">Set 1</td></tr><tr><td colspan="3"> Age</td></tr><tr><td>  7 d</td><td align="char" char=".">16</td><td align="char" char=".">19.27</td></tr><tr><td>  21 d</td><td align="char" char=".">16</td><td align="char" char=".">18.14</td></tr><tr><td> SEM</td><td/><td align="char" char=".">1.01</td></tr><tr><td> <italic>P</italic>-value</td><td/><td align="char" char=".">0.4396</td></tr><tr><td> Portion</td><td/><td/></tr><tr><td>  Flat</td><td align="char" char=".">16</td><td align="char" char=".">23.20</td></tr><tr><td>  Point</td><td align="char" char=".">16</td><td align="char" char=".">14.21</td></tr><tr><td> SEM</td><td/><td align="char" char=".">1.01</td></tr><tr><td> <italic>P</italic>-value</td><td/><td align="char" char=".">&lt; 0.0001</td></tr><tr><td colspan="3">Set 2</td></tr><tr><td colspan="3"> Age</td></tr><tr><td>  21 d</td><td align="char" char=".">16</td><td align="char" char=".">16.92</td></tr><tr><td>  35 d</td><td align="char" char=".">16</td><td align="char" char=".">17.60</td></tr><tr><td> SEM</td><td/><td align="char" char=".">1.06</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">0.6543</td></tr><tr><td> Portion</td><td/><td/></tr><tr><td>  Flat</td><td align="char" char=".">16</td><td align="char" char=".">22.05</td></tr><tr><td>  Point</td><td align="char" char=".">16</td><td align="char" char=".">12.47</td></tr><tr><td> SEM</td><td/><td align="char" char=".">1.06</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">&lt; 0.0001</td></tr><tr><td colspan="3">Set 3</td></tr><tr><td colspan="3"> Age</td></tr><tr><td>  7 d</td><td align="char" char=".">16</td><td align="char" char=".">17.99</td></tr><tr><td>  35 d</td><td align="char" char=".">16</td><td align="char" char=".">17.16</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.93</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">0.5297</td></tr><tr><td> Portion</td><td/><td/></tr><tr><td>  Flat</td><td align="char" char=".">16</td><td align="char" char=".">22.95</td></tr><tr><td>  Point</td><td align="char" char=".">16</td><td align="char" char=".">12.20</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.93</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">&lt; 0.0001</td></tr></tbody></table><table-wrap-foot><fn><p><sup>1</sup>Number of observations evaluated.</p></fn></table-wrap-foot></table-wrap><p>For all treatment comparison sets, point portions had lower (<italic>P</italic> &lt; 0.05) WBS force values than flat portions, regardless of length of age (<xref ref-type="table" rid="tbl4">Table 4</xref>). This is consistent with the findings of <xref ref-type="bibr" rid="bib1">Belew et al. (2003)</xref> and <xref ref-type="bibr" rid="bib8">Johnson et al. (1988)</xref>. In contrast, <xref ref-type="bibr" rid="bib11">Ramsbottom et al. (1945)</xref> reported the flat as having lower shear force values than the point. This inconsistency could be attributed to the varying cook methods and postmortem aging durations among these studies. <xref ref-type="bibr" rid="bib11">Ramsbottom et al. (1945)</xref> cooked the brisket portions in lard to an internal temperature of 76.7°C, and <xref ref-type="bibr" rid="bib8">Johnson et al. (1988)</xref> cooked briskets to 70°C in a water bath. In contrast, we used a commercial pit barbecue smoker to cook product to approximately 85°C. Additionally, the briskets evaluated by <xref ref-type="bibr" rid="bib11">Ramsbottom et al. (1945)</xref> and <xref ref-type="bibr" rid="bib8">Johnson et al. (1988)</xref> were aged 5 d and 21 d, respectively, compared to the 7 d, 21 d, and 35 d aging periods we used. These factors created differences among the studies that are important to consider when comparing results.</p></sec><sec><title>Consumer sensory evaluations</title><p>There were no (<italic>P</italic> &gt; 0.05) interactions between aging treatment and portion for the consumer sensory traits for any of the 3 sets (data not presented in tabular form). Least squares means for rankings in overall liking, flavor liking, tenderness liking, and juiciness liking for Set 1, 2, and 3 are reported in <xref ref-type="table" rid="tbl5">Table 5</xref>. Consumer ratings did not differ (<italic>P</italic> &gt; 0.05) between aging treatments within each comparison set. However, consumers did detect differences (<italic>P</italic> &lt; 0.05) between point and flat portions within each comparison.</p><table-wrap id="tbl5"><label>Table 5.</label><caption><p>Least squares means and SEM for consumer sensory rankings of beef palatability attributes for Sets 1<sup>1</sup>, 2<sup>1</sup>, and 3<sup>1</sup> stratified by aging treatment and brisket portion main effects</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Aging treatment and brisket portion comparison</td><td align="center"><italic>n</italic><sup>2</sup></td><td align="center">Overall like/dislike<sup>3</sup></td><td align="center">Flavor like/dislike<sup>3</sup></td><td align="center">Tenderness like/dislike<sup>3</sup></td><td align="center">Juiciness like/dislike<sup>3</sup></td></tr></thead><tbody><tr><td colspan="6">Set 1</td></tr><tr><td colspan="6"> Age</td></tr><tr><td>  7 d</td><td align="char" char=".">16</td><td align="char" char=".">6.3</td><td align="char" char=".">6.6</td><td align="char" char=".">6.2</td><td align="char" char=".">6.0</td></tr><tr><td>  21 d</td><td align="char" char=".">16</td><td align="char" char=".">6.9</td><td align="char" char=".">6.9</td><td align="char" char=".">6.8</td><td align="char" char=".">6.5</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.21</td><td align="char" char=".">0.18</td><td align="char" char=".">0.28</td><td align="char" char=".">0.23</td></tr><tr><td> <italic>P</italic>-value</td><td/><td align="char" char=".">0.0818</td><td align="char" char=".">0.1728</td><td align="char" char=".">0.1456</td><td align="char" char=".">0.1393</td></tr><tr><td> Portion</td><td/><td/><td/><td/><td/></tr><tr><td>  Flat</td><td align="char" char=".">16</td><td align="char" char=".">6.8</td><td align="char" char=".">7.0a</td><td align="char" char=".">6.2</td><td align="char" char=".">5.6b</td></tr><tr><td>  Point</td><td align="char" char=".">16</td><td align="char" char=".">6.4</td><td align="char" char=".">6.5b</td><td align="char" char=".">6.9</td><td align="char" char=".">6.9a</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.21</td><td align="char" char=".">0.18</td><td align="char" char=".">0.28</td><td align="char" char=".">0.23</td></tr><tr><td> <italic>P</italic>-value</td><td/><td align="char" char=".">0.1807</td><td align="char" char=".">0.0348</td><td align="char" char=".">0.0864</td><td align="char" char=".">0.0004</td></tr><tr><td colspan="6">Set 2</td></tr><tr><td colspan="6"> Age</td></tr><tr><td>  21 d</td><td align="char" char=".">16</td><td align="char" char=".">6.4</td><td align="char" char=".">6.9</td><td align="char" char=".">6.7</td><td align="char" char=".">6.6</td></tr><tr><td>  35 d</td><td align="char" char=".">16</td><td align="char" char=".">6.9</td><td align="char" char=".">7.3</td><td align="char" char=".">7.0</td><td align="char" char=".">6.6</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.24</td><td align="char" char=".">0.19</td><td align="char" char=".">0.26</td><td align="char" char=".">0.19</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">0.1381</td><td align="char" char=".">0.1073</td><td align="char" char=".">0.5269</td><td align="char" char=".">0.8533</td></tr><tr><td> Portion</td><td/><td/><td/><td/><td/></tr><tr><td>  Flat</td><td align="char" char=".">16</td><td align="char" char=".">7.0a</td><td align="char" char=".">7.4</td><td align="char" char=".">6.7</td><td align="char" char=".">5.8b</td></tr><tr><td>  Point</td><td align="char" char=".">16</td><td align="char" char=".">6.3b</td><td align="char" char=".">6.8</td><td align="char" char=".">7.1</td><td align="char" char=".">7.3a</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.24</td><td align="char" char=".">0.19</td><td align="char" char=".">0.26</td><td align="char" char=".">0.19</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">0.0499</td><td align="char" char=".">0.0602</td><td align="char" char=".">0.2571</td><td align="char" char=".">&lt; .0001</td></tr><tr><td colspan="6">Set 3</td></tr><tr><td colspan="6"> Age</td></tr><tr><td>  7 d</td><td align="char" char=".">16</td><td align="char" char=".">6.8</td><td align="char" char=".">7.1</td><td align="char" char=".">6.8</td><td align="char" char=".">6.6</td></tr><tr><td>  35 d</td><td align="char" char=".">16</td><td align="char" char=".">6.4</td><td align="char" char=".">6.8</td><td align="char" char=".">6.7</td><td align="char" char=".">6.4</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.22</td><td align="char" char=".">0.22</td><td align="char" char=".">0.24</td><td align="char" char=".">0.22</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">0.1719</td><td align="char" char=".">0.3031</td><td align="char" char=".">0.6498</td><td align="char" char=".">0.4669</td></tr><tr><td> Portion</td><td/><td/><td/><td/><td/></tr><tr><td>  Flat</td><td align="char" char=".">16</td><td align="char" char=".">7.0a</td><td align="char" char=".">7.2</td><td align="char" char=".">6.5</td><td align="char" char=".">6.1b</td></tr><tr><td>  Point</td><td align="char" char=".">16</td><td align="char" char=".">6.2b</td><td align="char" char=".">6.7</td><td align="char" char=".">6.9</td><td align="char" char=".">6.9a</td></tr><tr><td> SEM</td><td/><td align="char" char=".">0.22</td><td align="char" char=".">0.22</td><td align="char" char=".">0.24</td><td align="char" char=".">0.22</td></tr><tr><td> <italic>P-</italic>value</td><td/><td align="char" char=".">0.0296</td><td align="char" char=".">0.1028</td><td align="char" char=".">0.2285</td><td align="char" char=".">0.0112</td></tr></tbody></table><table-wrap-foot><fn><p><sup>1</sup>Set 1: 7 d versus 21 d aging; Set 2: 21 d versus 35 d aging; Set 3: 7 d versus 35 d aging.</p></fn><fn><p><sup>2</sup>Number of observations evaluated.</p></fn><fn><p><sup>3</sup>Rankings were assigned based on a nine-point hedonic scale for each attribute (1 = dislike extremely and 9 = like extremely).</p></fn></table-wrap-foot></table-wrap><p>Overall liking ratings were greater for the flat compared to the point sections in Set 2 (<italic>P</italic> = 0.0499) and Set 3 (<italic>P</italic> = 0.0296). Flavor liking ratings for flat portions within Set 1 were higher (<italic>P</italic> = 0.0348) than point portions, although there were no (<italic>P</italic> &gt; 0.05) flavor attribute differences found for the 2 portions in sets 2 and 3. <xref ref-type="bibr" rid="bib7">Jeremiah et al. (2003)</xref> found the point and flat portion flavor intensity and desirability ratings to reside between 4 and 6 on a 9-point hedonic scale, and the flat portion had higher scores than the point. The values in the past study were still lower and less desirable than the values found in our study. In addition, there are multiple influences on flavor in beef, one in particular being lipid type, amount, and composition (<xref ref-type="bibr" rid="bib18">Wood et al., 2004</xref>). With regards to lipid type and amount, <xref ref-type="bibr" rid="bib10">Mason et al. (2009)</xref> dissected point and flat portions of the brisket to determine the percentages of external fat, seam fat, and extractable fat, finding the flat portion had a higher percent fat and lower percent lean when compared to the point. In addition, the USDA’s National Nutrient Database for Standard Reference (<xref ref-type="bibr" rid="bib16">USDA, 2016b</xref>) gives information on total lipid within each portion; on a raw basis, the flat portion has a higher fat content (22.18 g/100 g vs. 20.98 g/100 g) than the point. However, this is reversed on a cooked basis. Both resources show that there is variability in the fat content of the point and flat portions of beef brisket. Further studies should be conducted on the lipid composition of the point and flat portions and how this pertains to consumer preferences of each; this may help clarify the differences found in our study for flavor like/dislike.</p><p>Surprisingly, there were no (<italic>P</italic> &gt; 0.05) tenderness liking differences detected among the point and flat portions in any of the set comparisons, even though there were WBS value differences between the portions. Because the WBS force values fell below the “very tender” threshold defined by <xref ref-type="bibr" rid="bib1">Belew et al. (2003)</xref>, it may be that consumers were unable to detect slight differences in tenderness ratings between samples. In comparison, <xref ref-type="bibr" rid="bib7">Jeremiah et al. (2003)</xref>, using a trained sensory panel and a nine-point scale, found the tenderness rankings for both portions of the brisket to be slightly lower (∼5.5 to 6.0) than what we found.</p><p>Finally, it is interesting to note consumers preferred (<italic>P</italic> &lt; 0.05) the juiciness of point portions as compared to flat portions in all three comparison sets, paralleling what was found by <xref ref-type="bibr" rid="bib7">Jeremiah et al. (2003)</xref>. Interestingly, the preference in point portion juiciness seen in Set 2 and Set 3 did not drive overall like or dislike preferences, as flats obtained higher rankings in this category. This finding may indicate that consumers preferred samples from the leaner flat portion, even though they gave higher ratings for specific traits to samples from the point portion.</p><p>Although there were similarities between results from our study and those from <xref ref-type="bibr" rid="bib7">Jeremiah et al. (2003)</xref>, the differences seen are most likely attributable to the difference in sample preparation. <xref ref-type="bibr" rid="bib7">Jeremiah et al. (2003)</xref> roasted 6 d aged 1 kg roasts in an electric convection oven to 72°C; a method very different than smoking to higher temperatures (approx. 85°C) for longer times (∼11 h). It may be that cooking technique has a large effect on palatability for muscles such as those that make up the brisket. Furthermore, differences in sensory panel type could contribute to the differing results; a trained consumer panel was utilized by <xref ref-type="bibr" rid="bib7">Jeremiah et al. (2003)</xref>, whereas a consumer panel was used in our study.</p></sec><sec><title>Conclusions</title><p>Significant aging treatment differences were not found for the objective (WBS force) and subjective (consumer sensory panel) techniques used to assess palatability attributes. Therefore, if smoked briskets are prepared using a Texas-style barbecue method, cooking at low temperatures for long durations of time, no added palatability benefits would be achieved through using product with extended postmortem aging periods. However, based on WBS force and consumer differences detected between point and flat portions, pit masters and barbecue enthusiasts may find value in buying individual muscle pieces or marketing the 2 portions individually.</p><p>In addition, despite previous studies that ranked the brisket point and flat portion as being tough cuts/muscles, this study revealed there is an advantage to preparing briskets Texas-style, as the WBS force values fell well below the thresholds determined as “very tender” by previous studies, and palatability ratings were relatively high on average (6 and 7 on 9-point scale).</p></sec></sec></body><back><fn-group><fn><p>Funded, in part, by the Beef Checkoff. 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