<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD with OASIS Tables with MathML3 v1.2d1 20130915//EN" "JATS-archive-oasis-article1.dtd"><article article-type="research-article" xml:lang="en" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><front><journal-meta><journal-id journal-id-type="publisher-id">MMB</journal-id><journal-title-group><journal-title>Meat and Muscle Biology</journal-title></journal-title-group><issn pub-type="epub">2575-985X</issn><publisher><publisher-name>American Meat Science Association</publisher-name><publisher-loc/></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.22175/mmb.25038</article-id><article-id pub-id-type="publisher-id"/><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Association Between Beef Ribeye Area Measurements and Steak Portion Size</article-title><alt-title alt-title-type="right-running">Schiefelbein et al.&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;&#x02003;Ribeye area and portion size</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Schiefelbein</surname><given-names>Abbey F.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Smith</surname><given-names>Colton L.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Geornaras</surname><given-names>Ifigenia</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Scanga</surname><given-names>John A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Belk</surname><given-names>Keith E.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Delmore</surname><given-names>Robert J.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Clark</surname><given-names>Daniel L.</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Nair</surname><given-names>Mahesh N.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="aff1"><label><sup>1</sup></label>Department of Animal Sciences, <institution>Colorado State University</institution>, Fort Collins, CO 80523, USA</aff><aff id="aff2"><label><sup>2</sup></label><institution>Certified Angus Beef</institution>, Wooster, OH 44691, USA</aff></contrib-group><author-notes><corresp id="cor1"><label>&#x0002A;</label>Corresponding author. Email: <email>mnair@colostate.edu</email> (Mahesh N. Nair)</corresp></author-notes><pub-date date-type="epub" publication-format="electronic"><day>00</day><month>00</month><year>0000</year></pub-date><volume>10</volume><issue>1</issue><fpage>1</fpage><lpage>8</lpage><history><date date-type="received"><day>26</day><month>03</month><year>2026</year></date><date date-type="accepted"><day>25</day><month>06</month><year>2026</year></date></history><permissions><copyright-statement>&#x000A9; 2026 Schiefelbein, Smith, Geornaras, Scanga, Belk, Delmore, Clark, and Nair.</copyright-statement><copyright-year>2026</copyright-year><copyright-holder>&#x000A9; 2026 Schiefelbein, Smith, Geornaras, Scanga, Belk, Delmore, Clark, and Nair.</copyright-holder><license license-type="open-access"><license-p>This is an open access article distributed under the CC BY license (<ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</ext-link>)</license-p></license></permissions><abstract><title>Abstract</title><p>With the increase in cattle weights over the past decades, hot carcass weight and ribeye area (REA) have also increased, which, in turn, affects steak thickness. The objective of this study was to examine the relationship between REA and steak portion size and estimate the change in portion size based on REA. Beef carcasses (<italic>n</italic>&#x02009;&#x0003D;&#x02009;100) were selected based on REA in 10 categories of 6.45-cm<sup>2</sup> (1-in<sup>2</sup>) increments, ranging from less than 70.97&#x000A0;cm<sup>2</sup> (11 in<sup>2</sup>) to greater than 122.58&#x000A0;cm<sup>2</sup> (19 in<sup>2</sup>). The REA measurements were obtained using the grading camera, a manual grid, and tracing. Strip loins were collected from the selected carcasses, and their weight, length, and width were measured. Each strip loin was then scanned through a portion cutter to determine the thickness of 340.19-g (12-oz) and 453.59-g (16-oz) portions and the weight of a 2.54-cm (1-in) thick portion. Linear regression models were developed using traced REA to examine the relationship between steak thickness (cut to 12-oz and 16-oz portions) and steak weight (cut at 1-in thickness). Each model was evaluated separately with significance set at &#x003B1;&#x02009;&#x0003D;&#x02009;0.05. There was a linear relationship (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.05) between traced REA measurement and 12-oz (<italic>R</italic><sup>2</sup>&#x02009;&#x0003D;&#x02009;&#x02212;0.71), 16-oz (<italic>R</italic><sup>2</sup>&#x02009;&#x0003D;&#x02009;&#x02212;0.71), and 1-in-thick (<italic>R</italic><sup>2</sup>&#x02009;&#x0003D;&#x02009;0.75) portions. For 340.19-g (12-oz) steaks, the thickness decreased by an estimated 0.138&#x000A0;cm (0.054 in), whereas the thickness of the 453.59-g (16-oz) steaks decreased by an estimated 0.185&#x000A0;cm (0.073 in) for every 6.45-cm<sup>2</sup> (1-in<sup>2</sup>) increase in REA. The 2.54-cm (1-in) steak portions had a mean weight of 340 g, and the steak weight increased by 18 g for every 6.45-cm<sup>2</sup> (1-in<sup>2</sup>) increase in REA. Overall, these results indicate that REA had a linear relationship with the portion size of strip loin steaks cut to a specified weight or thickness and provide quantitative estimates of the corresponding differences in portion dimensions.</p></abstract><kwd-group><title>Keywords:</title><kwd>beef</kwd><kwd>ribeye area</kwd><kwd>steak thickness</kwd></kwd-group></article-meta></front><body><sec id="sec1"><title>Introduction</title><p>Average beef carcass weights have continued to increase over the past several years (<xref ref-type="bibr" rid="r12">US Department of Agriculture [USDA] Economic Research Service, 2025</xref>). While this has led to a potential increase in efficiency during beef processing, it has also led beef processors to apply some discounts on beef prices (when purchased on a grid-based marketing system) when carcasses are over 408&#x000A0;kg and additional discounts for carcasses over 476&#x000A0;kg (<xref ref-type="bibr" rid="r14">USDA Economics, Statistics and Market Information System, 2024a</xref>). Along with the increase in the hot carcass weight (HCW), the average ribeye area (REA) has also increased over the years. Specifically, the 2022 National Beef Quality Audit indicated that the average HCW has increased by 56.9&#x000A0;kg from 1991 (average HCW of 345.0&#x000A0;kg) to 2022 (average HCW of 401.9&#x000A0;kg), while the REA increased by 7.6&#x000A0;cm<sup>2</sup> (1.2 in<sup>2</sup>) from 1991 with the average REA of 91.0&#x000A0;cm<sup>2</sup> (14.1 in<sup>2</sup>) in 2022 (<xref ref-type="bibr" rid="r7">Lorenzen et&#x000A0;al., 1993</xref>; <xref ref-type="bibr" rid="r9">Mayer et&#x000A0;al., 2024</xref>). In general, REA is used to calculate the yield grade to predict cutability, but it can also affect the thickness of whole-muscle steaks if they are portioned to a specific weight.</p><p>Previous consumer surveys have reported that consumers prefer to purchase thicker steaks when comparing 0.5-in, 1.0-in, and 1.5-in thick ribeye and sirloin steaks (<xref ref-type="bibr" rid="r8">Maples et&#x000A0;al., 2018</xref>). Moreover, steak thickness can influence the consumer&#x02019;s eating experience. Miller et&#x000A0;al. (<xref ref-type="bibr" rid="r10">2019</xref>) evaluated the interaction between steak thickness and grill temperature on consumers&#x02019; perceived liking and flavor of beef top loin steaks and reported that thickness impacted beef flavor with thick steaks (3.8&#x000A0;cm) cooked at low temperatures (177&#x000B0;C) or thin steaks (1.3&#x000A0;cm) cooked at high temperatures (232&#x000B0;C), having more positive sensory traits and consumer liking scores. Similarly, Kerth (<xref ref-type="bibr" rid="r4">2016</xref>) reported that the steak thickness influenced the generation of volatile compounds during cooking, with thin steaks (1.27&#x000A0;cm) producing volatiles originating from lipid degradation, whereas thick steaks (3.8&#x000A0;cm) produced more Maillard products. Differences in the volatile compounds generated could affect perceived flavor, thereby altering the overall liking of the steak. Additionally, Dunn et&#x000A0;al. (<xref ref-type="bibr" rid="r2">2000</xref>) reported that steak thickness affected consumer perception and eating quality, with thicker steaks having a more intense flavor compared to thinner steaks.</p><p>Due to this association among REA, steak thickness, and eating quality, previous studies have attempted to utilize the REA to predict steak thickness (<xref ref-type="bibr" rid="r1">Bass et&#x000A0;al., 2009</xref>; <xref ref-type="bibr" rid="r11">Steele et&#x000A0;al., 2020</xref>). Steele et&#x000A0;al. (<xref ref-type="bibr" rid="r11">2020</xref>) reported that the strip loins from the smaller REA categories were more likely to meet the optimal weight and thickness; however, sorting carcasses by REA was less associated with the tenderloin weight and thickness than it was for ribeye or strip loins, suggesting that sorting subprimals based on REA might improve boxed product consistency. Bass et&#x000A0;al. (<xref ref-type="bibr" rid="r1">2009</xref>) also observed similar results, with only 7 of the 14 muscles examined showing direct effects of the REA on their retail portion weights. These authors reported that the portion characteristics for longissimus thoracis (ribeye) had moderate to low correlations with carcass REA, and portion characteristics for the longissimus lumborum (strip loin) had weak to very weak correlations with carcass REA.</p><p>The REA is also used as a metric for inclusion into several branded beef programs in the United States, which could offer a higher price for carcasses that meet their specifications. For example, the Certified Angus Beef program specifies that carcasses should have a REA between 64.52&#x000A0;cm<sup>2</sup> (10 in<sup>2</sup>) and 109.68&#x000A0;cm<sup>2</sup> (17 in<sup>2</sup>) based on measurements taken from the grading camera to be included in the brand&#x02019;s program. Therefore, understanding the relationship between REA and steak thickness/weight is critical to ensure the consistency of beef products. The objective of this study was to evaluate the effect of differing REA on the steak dimension of longissimus lumborum muscle cut to various weights (340.19 g [12&#x000A0;oz] and 453.59 g [16&#x000A0;oz]) or thickness (2.54&#x000A0;cm [1 in]) and to estimate the change in portion size based on REA.</p></sec><sec id="sec2"><title>Materials and Methods</title><sec id="sec2.1"><title>Carcass selection</title><p>Beef carcasses (<italic>n</italic>&#x02009;&#x0003D;&#x02009;100) were selected from a commercial beef harvesting facility based on REA, categorized as 6.45-cm<sup>2</sup> (1-in<sup>2</sup>) increments ranging from less than 70.97&#x000A0;cm<sup>2</sup> (11 in<sup>2</sup>) to greater than 122.58&#x000A0;cm<sup>2</sup> (19 in<sup>2</sup>) based on a USDA-approved camera (E&#x0002B;V) with 10 total categories. Carcass collection was intended to achieve an even distribution of carcasses in each REA category. Instrumental carcass data, including the HCW, marbling score, and fat thickness, were obtained from the facility. In addition to the REA obtained from the grading camera, REA was also measured using an approved USDA ribeye grid and tracing. The traced REA measurement was accomplished using plain paper copier transparency film (APOLLO, IL, item #VPP100C) and a permanent marker, which was used to outline the REA. Tracings were scanned into the computer as .jpg images with a resolution of 1120&#x02009;&#x000D7;&#x02009;1696 pixels per image. ImageJ software (Image J 1.54d, Java 1.8.0_345) was calibrated to 1.001 in<sup>2</sup> with a 1-in<sup>2</sup> rectangle (1 in&#x02009;&#x0003D;&#x02009;208.36 pixels) to calculate the REA. After calibration, the software processed each tracing to estimate the REA.</p></sec><sec id="sec2.2"><title>Strip loin collection</title><p>The selected beef carcasses were fabricated in the processing facility, and strip loins (IMPS#180) from the right side were collected from each carcass. Strip loins were then trimmed to approximately 0.64&#x000A0;cm (0.25 in) of external fat and vacuum packaged. Each strip loin was boxed and transported to Marel Center (Marel, Des Moines, Iowa), where they were held under refrigeration overnight.</p></sec><sec id="sec2.3"><title>Dimension data collection</title><p>The external fat of each strip loin was further trimmed for uniformity to approximately 0.32-cm (0.125-in) thickness. Then, the weight (g), length (cm; anterior to posterior), and 3 width measurements (cm; anterior, midpoint, and posterior) were recorded manually using a tape measure. The strip loins were scanned using the Marel I-Cut 56 portion cutter (Marel), which used a laser to scan each strip loin and used the scan data to calculate weight or thickness. Individual strip loins were placed onto the belt with the fat cover facing the belt. The I-Cut 56 portion cutter mapped each strip loin&#x02019;s topical area, weight, length, and maximum height (cm). The machine was also programmed to estimate an initial facing cut of 50 g to help square the remainder of the portioned steaks. Each strip loin was scanned 3 times with the blades removed to allow for multiple scans to collect data for 340.19-g (12-oz), 453.59-g (16-oz), or 2.54-cm (1-in) thick portion sizes. Using an estimation for the density of fresh beef, the I-Cut would determine how thick each cut would have been if cuts were made to make 12-oz, 16-oz, or 1-in thick portions. Within the I-Cut software, the estimated outcomes for each portion&#x02019;s thickness or weight were recorded. The strip loin&#x02019;s maximum height (depth) was measured at each portion size and averaged for each strip loin at the end.</p></sec><sec id="sec2.4"><title>Statistical analysis</title><p>The Pearson correlation between REA and the 3 portion size data was calculated using the stats package in R (version 4.2.2). Two initial and final steak portions were excluded from the study to avoid potential bias from tapering at the anterior or posterior ends of the strip loins. The remaining portions of the steak&#x02019;s thickness or weight within a strip loin were averaged, and each strip loin was considered the experimental unit. All 3 REA measurement methods (camera, gridded, and traced) were highly correlated (Table&#x000A0;<xref ref-type="table" rid="tab1">1</xref>; <italic>P</italic>&#x02009;&#x0003C;&#x02009;.001). However, 34% of carcasses had a 2.54 cm (1 in) or greater difference between USDA-approved camera measurements and traced REA measurements. Therefore, the traced REA was selected as the predictor for all 3 portion sizes to develop the linear regression model, as it was obtained manually and could be considered the standard value. Each model was then evaluated separately for the main effects of each variable, with significance set at &#x003B1;&#x02009;&#x0003D;&#x02009;0.05.</p><table-wrap id="tab1"><label>Table 1.</label><caption><p>Pearson correlation between different methods for measuring the ribeye area (camera, gridded, and traced)</p></caption><table><colgroup><col align="left"/><col align="char" char="."/><col align="char" char="."/><col align="center"/></colgroup><thead><tr><th/><th>Camera REA</th><th>Gridded REA</th><th>Traced REA</th></tr></thead><tbody><tr><td>Camera REA</td><td>1.000</td><td/><td/></tr><tr><td>Gridded REA</td><td>0.905<xref ref-type="table-fn" rid="tab1fn2"><sup>&#x0002A;</sup></xref></td><td>1.000</td><td/></tr><tr><td>Traced REA</td><td>0.898<xref ref-type="table-fn" rid="tab1fn2"><sup>&#x0002A;</sup></xref></td><td>0.982<xref ref-type="table-fn" rid="tab1fn2"><sup>&#x0002A;</sup></xref></td><td>1.000</td></tr></tbody></table><table-wrap-foot><fn id="tab1fn1"><p>REA, ribeye area.</p></fn><fn id="tab1fn2"><label><sup>&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.001.</p></fn></table-wrap-foot></table-wrap></sec></sec><sec id="sec3"><title>Results</title><p>One hundred carcasses were selected based on USDA-approved camera REA, with REA ranging from 63.2 to 125.8&#x000A0;cm<sup>2</sup> (9.8&#x02013;19.5 in<sup>2</sup>). The distribution of carcasses in each REA bracket is presented in Figure&#x000A0;<xref ref-type="fig" rid="f1">1</xref>. Even though the intent was to obtain 10 carcasses in each category, there were slightly lower numbers of carcasses in the less-than-70.97-cm<sup>2</sup> (11-in<sup>2</sup>; <italic>n</italic>&#x02009;&#x0003D;&#x02009;7) and greater-than-122.58-cm<sup>2</sup> (19-in<sup>2</sup>; <italic>n</italic>&#x02009;&#x0003D;&#x02009;6) categories. Descriptive summary statistics of the carcass data collected are presented in Table&#x000A0;<xref ref-type="table" rid="tab2">2</xref>. The average REA from a USDA-approved camera measurement, gridded measurement, and traced measurement were 94.8&#x000A0;cm<sup>2</sup> (14.7 in<sup>2</sup>), 96.8&#x000A0;cm<sup>2</sup> (15.0 in<sup>2</sup>), and 93.5&#x000A0;cm<sup>2</sup> (14.5 in<sup>2</sup>), respectively (Table&#x000A0;<xref ref-type="table" rid="tab2">2</xref>). The average HCW was 399.0&#x02009;&#x000B1;&#x02009;66.0&#x000A0;kg with a minimum of 262.0&#x000A0;kg and a maximum of 534.0&#x000A0;kg. Fat thickness also varied, ranging from 0.8 to 3.3&#x000A0;cm. Furthermore, the average strip loin weight was 5.3&#x02009;&#x000B1;&#x02009;1.0&#x000A0;kg with an average length of 41.4&#x02009;&#x000B1;&#x02009;2.6&#x000A0;cm and an average height of 10.0&#x02009;&#x000B1;&#x02009;0.1&#x000A0;cm (Table&#x000A0;<xref ref-type="table" rid="tab2">2</xref>).</p><fig id="f1"><label>Figure 1.</label><caption><p>Number of beef strip loins (<italic>n</italic>&#x02009;&#x0003D;&#x02009;100) within each category of the incremental ribeye area category increasing by 1 in<sup>2</sup> from less than 71.0&#x000A0;cm<sup>2</sup> (&#x0003C;11 in<sup>2</sup>) through greater than 122.6&#x000A0;cm<sup>2</sup> (&#x0003E;19.0 in<sup>2</sup>). REA, ribeye area.</p></caption><graphic xlink:href="1.png"/></fig><table-wrap id="tab2"><label>Table 2.</label><caption><p>Descriptive summary statistics for data collected from beef carcasses (<italic>n</italic>&#x02009;&#x0003D;&#x02009;100)</p></caption><table><colgroup><col align="left"/><col align="center"/><col align="char" char="."/><col align="char" char="."/><col align="char" char="."/></colgroup><thead><tr><th>Characteristic</th><th align="center">Mean (&#x000B1;SD)</th><th align="center">Minimum</th><th align="center">Median</th><th align="center">Maximum</th></tr></thead><tbody><tr><td>Camera REA (cm<sup>2</sup>)</td><td>94.8&#x02009;&#x000B1;&#x02009;17.4</td><td>63.2</td><td>93.5</td><td>125.8</td></tr><tr><td>Gridded REA (cm<sup>2</sup>)</td><td>96.8&#x02009;&#x000B1;&#x02009;15.5</td><td>67.7</td><td>96.1</td><td>126.5</td></tr><tr><td>Traced REA (cm<sup>2</sup>)</td><td>93.5&#x02009;&#x000B1;&#x02009;15.5</td><td>66.5</td><td>92.3</td><td>124.5</td></tr><tr><td>HCW (kg) (<italic>n</italic>&#x02009;&#x0003D;&#x02009;98)<xref ref-type="table-fn" rid="tab2fn2"><sup>1</sup></xref></td><td>399.0&#x02009;&#x000B1;&#x02009;66.0</td><td>262.0</td><td>407.0</td><td>534.0</td></tr><tr><td>Carcass fat thickness<xref ref-type="table-fn" rid="tab2fn3"><sup>2</sup></xref> (cm) (<italic>n</italic>&#x02009;&#x0003D;&#x02009;98)<xref ref-type="table-fn" rid="tab2fn2"><sup>1</sup></xref></td><td>1.7&#x02009;&#x000B1;&#x02009;0.5</td><td>0.8</td><td>1.6</td><td>3.3</td></tr><tr><td>Marbling score (<italic>n</italic>&#x02009;&#x0003D;&#x02009;98)<xref ref-type="table-fn" rid="tab2fn2"><sup>1</sup></xref></td><td>503.0&#x02009;&#x000B1;&#x02009;73.0</td><td>398.0</td><td>486.0</td><td>727.0</td></tr><tr><td>Strip loin weight (kg)</td><td>5.3&#x02009;&#x000B1;&#x02009;1.0</td><td>3.3</td><td>5.3</td><td>7.4</td></tr><tr><td>Strip loin length (cm)</td><td>41.4&#x02009;&#x000B1;&#x02009;2.6</td><td>35.0</td><td>41.5</td><td>47.0</td></tr><tr><td>Strip loin anterior width (cm)</td><td>21.0&#x02009;&#x000B1;&#x02009;1.9</td><td>15.0</td><td>21.0</td><td>26.0</td></tr><tr><td>Strip loin middle width (cm) (<italic>n</italic>&#x02009;&#x0003D;&#x02009;99)<xref ref-type="table-fn" rid="tab2fn2"><sup>1</sup></xref></td><td>20.7&#x02009;&#x000B1;&#x02009;1.8</td><td>17.0</td><td>20.0</td><td>25.0</td></tr><tr><td>Strip loin posterior width (cm)</td><td>24.1&#x02009;&#x000B1;&#x02009;2.1</td><td>19.0</td><td>24.0</td><td>30.0</td></tr><tr><td>Strip loin height (cm)</td><td>10.0&#x02009;&#x000B1;&#x02009;0.1</td><td>7.7</td><td>10.0</td><td>11.6</td></tr></tbody></table><table-wrap-foot><fn id="tab2fn1"><p>HCW, hot carcass weight; REA, ribeye area; SD, standard deviation from the mean.</p></fn><fn id="tab2fn2"><label><sup>1</sup></label><p><italic>n</italic> differs from the original dataset due to the exclusion of 2 observations without matching plant data and 1 observation with an implausible strip loin thickness value (8.5&#x000A0;cm).</p></fn><fn id="tab2fn3"><label><sup>2</sup></label><p>Fat thickness&#x02009;&#x0003D;&#x02009;subcutaneous fat thickness measured at 3/4 the length of the ribeye on the lateral side.</p></fn></table-wrap-foot></table-wrap><p>The mean thickness or weight of the portioned cuts is shown in Table&#x000A0;<xref ref-type="table" rid="tab3">3</xref>. The mean thickness of the 340.19-g (12-oz) portion was 2.62&#x02009;&#x000B1;&#x02009;0.39&#x000A0;cm (1.03 in), whereas the thickness of the 453.59-g (16-oz) portion was 3.53&#x02009;&#x000B1;&#x02009;0.52&#x000A0;cm (1.39 in). The 2.54-cm (1-in) thick portion had a mean weight of 340&#x02009;&#x000B1;&#x02009;50 g (12 &#x000B1; 1.76 oz). When evaluating the relationship between traced REA and average thickness of the portioned cuts, both the thickness of 12-oz (340.19-g) and 16-oz (453.29-g) portioned cuts were negatively correlated with the traced REA measurement (<italic>R</italic><sup>2</sup>&#x02009;&#x0003D;&#x02009;&#x02212;0.71, <italic>P</italic>&#x02009;&#x0003C;&#x02009;.001; Figure&#x000A0;<xref ref-type="fig" rid="f2">2</xref>), which means that as the REA increased, steak thickness decreased. Conversely, the average weight of each strip loin, when portioned to 1-in (2.54-cm) thick cuts, was positively correlated with traced REA (<italic>R</italic><sup>2</sup>&#x02009;&#x0003D;&#x02009;0.75, <italic>P</italic>&#x02009;&#x0003C;&#x02009;.001; Figure&#x000A0;<xref ref-type="fig" rid="f2">2</xref>), which means that the average weight of the 1-in thick steaks increased with the increase in REA.</p><table-wrap id="tab3"><label>Table 3.</label><caption><p>The mean (&#x000B1;SE), minimum, median, and maximum thickness or weight of the 3 different portioned sizes from beef strip loins (<italic>n</italic>&#x02009;&#x0003D;&#x02009;100) from various ribeye areas</p></caption><table><colgroup><col align="left"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/></colgroup><thead><tr><th>Portion Size</th><th align="center">Mean (&#x000B1;SE)</th><th align="center">Minimum</th><th align="center">Median</th><th align="center">Maximum</th></tr></thead><tbody><tr><td>340.19-g (12-oz) portion thickness (cm)</td><td>2.62&#x02009;&#x000B1;&#x02009;0.39</td><td>1.95</td><td>2.58</td><td>3.73</td></tr><tr><td>453.59-g (16-oz) portion thickness (cm)</td><td>3.53&#x02009;&#x000B1;&#x02009;0.52</td><td>2.60</td><td>3.48</td><td>4.95</td></tr><tr><td>2.54-cm (1-in) portion weight (g)</td><td>340.00&#x02009;&#x000B1;&#x02009;50</td><td>230</td><td>330</td><td>440</td></tr></tbody></table><table-wrap-foot><fn id="tab3-fn1"><p>SE, standard error.</p></fn></table-wrap-foot></table-wrap><fig id="f2"><label>Figure 2.</label><caption><p>Correlation between traced ribeye measurements (traced ribeye area) and 3 separate portion cut sizes: 12&#x000A0;oz (340.19g), 16&#x000A0;oz (453.59 g), and 1 in (2.54&#x000A0;cm; <italic>n</italic>&#x02009;&#x0003D;&#x02009;100). (A)&#x000A0;Traced REA vs the average thickness of a 12-oz portion (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.001), (B)&#x000A0;traced REA vs the average thickness of a 16-oz portion (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.001), and (C)&#x000A0;traced REA vs the average weight of a 1-in thick portion (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.001). REA, ribeye area.</p></caption><graphic xlink:href="2.png"/></fig><p>The correlation matrix for all factors measured in this study is presented in Table&#x000A0;<xref ref-type="table" rid="tab4">4</xref>. Traced REA was correlated (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.05) to all the parameters evaluated, except the marbling score and fat thickness. Similarly, HCW was correlated with all measurements (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.05), except the marbling score. The strip loin length and all measured widths (anterior, middle, and posterior) were also highly correlated with steak thickness and weight (<italic>P</italic> <italic>&#x0003C;</italic> .05).</p><table-wrap id="tab4"><label>Table 4.</label><caption><p>Pearson correlations between all measurements at carcass level (traced ribeye area, hot carcass weight, fat thickness, marbling score), strip loin level (weight, length, anterior width, middle width, posterior width), and portion size level (12&#x000A0;oz, 16&#x000A0;oz, and 1 in)</p></caption><table><colgroup><col align="left"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/></colgroup><thead><tr><th/><th align="center">Traced REA (in<sup>2</sup>)</th><th align="center">HCW (kg)</th><th align="center">Fat Thickness<xref ref-type="table-fn" rid="tab4fn2"><sup>1</sup></xref> (cm)</th><th align="center">Marbling Score</th><th align="center">Weight (kg)</th><th align="center">Strip Loin Length (cm)</th><th align="center">Anterior Width (cm)</th><th align="center">Middle Width (cm)</th><th align="center">Posterior Width (cm)</th><th align="center">12-oz Portion (cm)</th><th align="center">16-oz Portion (cm)</th></tr></thead><tbody><tr><td>HCW (kg)</td><td>0.836<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/><td/><td/><td/><td/><td/><td/><td/><td/></tr><tr><td>Fat thickness<xref ref-type="table-fn" rid="tab4fn2"><sup>1</sup></xref> (cm)</td><td>&#x02212;0.058</td><td>0.230<xref ref-type="table-fn" rid="tab4fn3"><sup>&#x0002A;</sup></xref></td><td/><td/><td/><td/><td/><td/><td/><td/><td/></tr><tr><td>Marbling score</td><td>&#x02212;0.083</td><td>0.209</td><td>0.350<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/><td/><td/><td/><td/><td/><td/></tr><tr><td>Weight (kg)</td><td>0.867<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.875<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.089</td><td>&#x02212;0.093</td><td/><td/><td/><td/><td/><td/><td/></tr><tr><td>Strip loin length (cm)</td><td>0.581<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.732<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.127</td><td>&#x02212;0.066</td><td>0.773<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/><td/><td/><td/><td/></tr><tr><td>Anterior width (cm)</td><td>0.790<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.699<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.016</td><td>&#x02212;0.011</td><td>0.740<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.473<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/><td/><td/><td/></tr><tr><td>Middle width (cm)</td><td>0.688<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.645<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.008</td><td>&#x02212;0.165</td><td>0.771<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.497<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.660<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/><td/><td/></tr><tr><td>Posterior width (cm)</td><td>0.624<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.694<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.004</td><td>&#x02212;0.066</td><td>0.796<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.597<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.571<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.662<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/><td/></tr><tr><td>12-oz portion (cm)</td><td>&#x02212;0.843<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.826<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.084</td><td>0.108</td><td>&#x02212;0.950<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.608<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.733<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.795<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.764<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/><td/></tr><tr><td>16-oz portion (cm)</td><td>&#x02212;0.843<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.832<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.090</td><td>0.102</td><td>&#x02212;0.954<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.619<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.734<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.786<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.770<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.998<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td/></tr><tr><td>1-in portion (g)</td><td>0.864<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.824<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.094</td><td>&#x02212;0.082</td><td>0.964<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.610<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.732<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.786<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>0.787<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.985<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td><td>&#x02212;0.986<xref ref-type="table-fn" rid="tab4fn5"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td></tr></tbody></table><table-wrap-foot><fn id="tab4fn1"><p>HCW, hot carcass weight; REA, ribeye area.</p></fn><fn id="tab4fn2"><label><sup>1</sup></label><p>Fat thickness&#x02009;&#x0003D;&#x02009;subcutaneous fat thickness measured at 3/4 the length of the ribeye on the lateral side.</p></fn><fn id="tab4fn3"><label><sup>&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.05.</p></fn><fn id="tab4fn4"><label><sup>&#x0002A;&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.01.</p></fn><fn id="tab4fn5"><label><sup>&#x0002A;&#x0002A;&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.001.</p></fn></table-wrap-foot></table-wrap><p>The results of the linear regression models indicated that traced REA was significant (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.001) for estimating all 3 portion sizes (Table&#x000A0;<xref ref-type="table" rid="tab5">5</xref>). A 2.54-cm (1-in) increase in REA was estimated to decrease the thickness of a 340.19-g (12-oz) portion and a 453.59-g (16-oz) portion by 0.138&#x000A0;cm (95% CI: &#x02212;0.155, &#x02212;0.120&#x000A0;cm) and 0.185&#x000A0;cm (95% CI: &#x02212;0.209, &#x02212;0.162&#x000A0;cm), respectively. Additionally, a 1-in increase in traced REA could increase the weight of a 1-in thick portion by 18 g (95% CI: 15, 20 g).</p><table-wrap id="tab5"><label>Table 5.</label><caption><p>Linear regression models for each portion measurement with the average thickness (cm) of 12-oz (340.19-g) or 16-oz (453.59-g) portioned cuts or the average weight (kg) of 1-in (2.54-cm) portion cuts (n&#x02009;&#x0003D;&#x02009;100) as the expected outcome, using the traced ribeye area measurement (in<sup>2</sup>) as the predictor</p></caption><table><colgroup><col align="left"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/><col align="center"/></colgroup><thead><tr><th>Predictor Variable</th><th align="center">Model Outcome</th><th align="center">Estimate&#x02009;&#x000B1;&#x02009;SE</th><th align="center">Lower CI</th><th align="center">Upper CI</th><th align="center">Test Statistic</th></tr></thead><tbody><tr><td>Traced REA</td><td>12-oz (340.19-g) portion (cm)</td><td>&#x02212;0.138&#x02009;&#x000B1;&#x02009;0.009</td><td>&#x02212;0.155</td><td>&#x02212;0.120</td><td>&#x02212;15.5<xref ref-type="table-fn" rid="tab5fn4"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td></tr><tr><td>Traced REA</td><td>16-oz (453.59-g) portion (cm)</td><td>&#x02212;0.185&#x02009;&#x000B1;&#x02009;0 .012</td><td>&#x02212;0.209</td><td>&#x02212;0.162</td><td>&#x02212;15.5<xref ref-type="table-fn" rid="tab5fn4"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td></tr><tr><td>Traced REA</td><td>1-in (2.54-cm) portion (g)</td><td>18.0&#x02009;&#x000B1;&#x02009;1.0</td><td>15.0</td><td>20.0</td><td>17.0<xref ref-type="table-fn" rid="tab5fn4"><sup>&#x0002A;&#x0002A;&#x0002A;</sup></xref></td></tr></tbody></table><table-wrap-foot><fn id="tab5fn1"><p>REA, ribeye area; SE, standard error.</p></fn><fn id="tab5fn2"><label><sup>&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.05.</p></fn><fn id="tab5fn3"><label><sup>&#x0002A;&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.01</p></fn><fn id="tab5fn4"><label><sup>&#x0002A;&#x0002A;&#x0002A;</sup></label><p><italic>P</italic>&#x02009;&#x0003C;&#x02009;.001.</p></fn></table-wrap-foot></table-wrap></sec><sec id="sec4"><title>Discussion</title><p>Steak thickness and steak weight can influence both food-service operations and retail merchandising. In food service, steaks are commonly purchased and marketed by weight. However, when steaks vary in thickness, achieving consistent degrees of doneness can be challenging because chefs often rely on cooking time to manage multiple steaks simultaneously. In response, chefs who fabricate steaks in-house may cut portions thicker, and therefore heavier than the advertised menu, to maintain a more premium visual appearance. Although this approach may enhance perceived quality and customer satisfaction, it can also reduce yield and negatively affect profitability. In retail settings, subprimals with large REA could create merchandising challenges. When steaks are cut to a thickness that meets consumer expectations, the resulting package price may exceed what many consumers are willing to pay. Conversely, cutting steaks thinner to maintain a lower package price can reduce visual appeal, leading consumers to select alternative packages with more desirable thickness.</p><p>Previous studies have demonstrated that the thickness of beef ribeye, top loin, and top sirloin steaks could be related to consumers&#x02019; willingness to pay (WTP; <xref ref-type="bibr" rid="r6">Leick et&#x000A0;al., 2012</xref>). Maples et&#x000A0;al. (<xref ref-type="bibr" rid="r8">2018</xref>) also examined the relationship between portion size and consumers&#x02019; WTP for beef steaks using ribeye and sirloin steaks with 3 different REA (10 in<sup>2</sup>, 14 in<sup>2</sup>, 18 in<sup>2</sup>) and 3 different thicknesses (0.5 in, 1.0 in, 1.5 in). These authors reported that 90% of consumers perceived the thinnest steaks (0.5 in) as less desirable compared to 1-in thick steaks. Additionally, consumers would pay $7.07 less per package for steaks with a 10-in<sup>2</sup> ribeye and $3.51 less per package for steaks with an 18-in<sup>2</sup> ribeye when compared to a 14-in<sup>2</sup> REA (<xref ref-type="bibr" rid="r8">Maples et&#x000A0;al., 2018</xref>). When assessing how steak thickness influenced consumers&#x02019; WTP, the authors found that WTP was $18.67 lower for 0.5-in thick steaks compared with 1-in thick steaks, while WTP increased by $4.66 for 1.5-in thick steaks relative to 1-in thick steaks (<xref ref-type="bibr" rid="r8">Maples et&#x000A0;al., 2018</xref>).</p><p>In the current study, the 340.19-g (12-oz) portion strip loin steaks had a mean thickness of 2.62&#x000A0;cm, whereas the 453.59-g (16-oz) portion had a mean thickness of 3.53&#x000A0;cm (Table&#x000A0;<xref ref-type="table" rid="tab3">3</xref>). Steak thickness can impact cookery and consumer perception. Thicker steaks had an overall higher consumer liking compared to thinner steaks when cooked the same way (<xref ref-type="bibr" rid="r10">Miller et&#x000A0;al., 2019</xref>), which could be due to differences in volatile compounds generated during cooking (<xref ref-type="bibr" rid="r4">Kerth, 2016</xref>). Specifically, Miller et&#x000A0;al. (<xref ref-type="bibr" rid="r10">2019</xref>) reported that thicker (3.8-cm) steaks cooked at grill surface temperature of 177&#x000B0;C had more intense umami basic taste and beef identity, whereas thinner (1.3-cm) steaks had lower levels of beef identity and brown/roasted flavor aromatics. These results indicated that consumers place high importance on steak thickness, which would be affected by the REA. Foster et&#x000A0;al. (<xref ref-type="bibr" rid="r3">2021</xref>) also reported that portioning steaks by a constant weight resulted in significant variations in thickness, regardless of HCW or REA, while steaks cut to a uniform thickness showed significant differences in weight. Additionally, these authors reported that the steak thickness affected shear force values and sensory attributes. When steaks were portioned by weight, those in the small REA category received the highest sensory overall liking and tenderness scores, outperforming steaks from the medium and large categories. No differences were observed among REA categories when steaks were portioned by thickness (<xref ref-type="bibr" rid="r3">Foster et&#x000A0;al., 2021</xref>). The difference in overall liking of steaks when portioned by weight (different thicknesses) indicates that sorting based on REA and portioning to a specific weight may lead to changes in overall liking of the steak.</p><p>The results of the current study also indicated that each of the 3 portion dimensions (12-oz, 16-oz, and 1-in thick) was correlated (<italic>P</italic>&#x02009;&#x0003C;&#x02009;.001; <italic>R</italic><sup>2</sup>&#x02009;&#x0003D;&#x02009;&#x02212;0.71, &#x02212;0.71, 0.75, respectively) to the traced REA measurements (Figure&#x000A0;<xref ref-type="fig" rid="f2">2</xref>). Dunn et&#x000A0;al. (<xref ref-type="bibr" rid="r2">2000</xref>) also categorized REA into 1-in<sup>2</sup> categories, ranging from less than 11 in<sup>2</sup> to greater than 16 in<sup>2</sup>, to examine the impact of REA on steak thickness, cooking parameters, and tenderness of the longissimus muscle in strip loin and T-bone steaks. As expected, steak thickness within groups portioned to a predetermined weight decreased with increasing REA, consistent with the results of the current study. These authors also indicated that REA affected tenderness and the cooking times of both strip steaks and T-bone steaks, with steaks portioned from carcasses with 77.4-cm<sup>2</sup> (11.99-in<sup>2</sup>) to 96.6-cm<sup>2</sup> (14.97-in<sup>2</sup>) REA having optimal tenderness and cooking times (<xref ref-type="bibr" rid="r2">Dunn et&#x000A0;al., 2000</xref>), which reiterates the importance of having consistent steak thickness for the food-service industry. On contrary, Bass et&#x000A0;al. (<xref ref-type="bibr" rid="r1">2009</xref>) reported that among the strip loin characteristics measured (whole-muscle maximum height, whole-muscle maximum width, whole-muscle maximum length, face length, and face width), only whole-muscle maximum height had a significant (still weak) correlation with REA. Previous studies attributed the variation in the correlation between muscle size and REA to differences in the breed of the cattle, sex, maturity, and management practices of the live animals (<xref ref-type="bibr" rid="r2">Dunn et&#x000A0;al., 2000</xref>; <xref ref-type="bibr" rid="r1">Bass et&#x000A0;al., 2009</xref>).</p><p>The REA is a key component of the current USDA yield grade equation used to estimate retail yield in beef carcasses. However, previous studies have discussed whether the original yield grade equations developed in 1965 should be modified to better reflect changes in beef carcass composition over time (<xref ref-type="bibr" rid="r5">Lawrence et&#x000A0;al., 2008</xref>). Although yield grade equations were not evaluated in the current study, our results indicate that a larger REA results in thinner boneless strip loin steaks when portioned to specific weights. A clearer understanding of the relationship between REA and subprimal muscling could provide additional context for future refining yield grade equations. Furthermore, advances in carcass sorting technologies and improved subprimal characterization could enable secondary sorting beyond the grading chain, allowing strip loins to be more effectively aligned with specific processing and end-use requirements.</p><p>In US meat processing facilities, sorting carcasses on the grading chain is essential because this is where premiums and discounts are assigned under grid-based pricing systems (<xref ref-type="bibr" rid="r13">USDA Economics, Statistics and Market Information System, 2024b</xref>). For example, carcasses that conform to boxed beef program standards can be rewarded with a premium because they can be marketed through higher value programs. The current study revealed that REA was an accurate predictor of steak thickness when portioned to 12 or 16&#x000A0;oz or to 1-in thickness. Numerous additional factors, including differences in production systems, cattle sex, age class, and breed, can affect predictors such as REA, HCW, and fat thickness across commercial beef populations. For example, Steele et&#x000A0;al. (<xref ref-type="bibr" rid="r11">2020</xref>) examined whether sorting by REA could be improved by sorting by the weight of the subprimal instead to increase the consistency of the product. These authors suggested that while weight may minimize some variation, it would not be fully sufficient to minimize most of the inter-carcass variation. This may be because these authors grouped subprimals into only 2 weight categories (heavy and light), whereas grouping into additional weight categories may reduce variability (<xref ref-type="bibr" rid="r11">Steele et&#x000A0;al., 2020</xref>).</p></sec><sec id="sec5"><title>Conclusions</title><p>The results of the current study indicated a linear relationship between REA and strip loin weight and thickness. Thickness of the strip loin steaks decreased by an estimated 0.138&#x000A0;cm (0.054 in.) for 340.19-g (12-oz) steaks and 0.185&#x000A0;cm (0.073 in.) for 453.59-g (16-oz) steaks per 6.45-cm<sup>2</sup> (1-in<sup>2</sup>) increase in REA. On the other hand, the weight of 2.54-cm (1-in) steak portions increased by approximately 18 g for every 6.45-cm<sup>2</sup> (1-in<sup>2</sup>) increase in REA. The industry could benefit from additional research to determine an optimal REA range based on consumer acceptance and degree of doneness for steaks with varying thickness, which could optimize consumer acceptance and steak portion sizes for retail and food service.</p></sec><sec id="sec6"><title>Conflict of Interest</title><p>The authors declare that there are no conflicts of interest relevant to this article.</p></sec></body><back><ack><title>Acknowledgments</title><p>This project was supported by funding from Certified Angus Beef. This work was also partially supported by the US Department of Agriculture National Institute of Food and Agriculture, Multistate Hatch project COL00276B (W5177).</p></ack><sec id="sec7"><title>Author Contribution</title><p>A. F. Schiefelbein: data collection, data analysis, investigation, writing&#x02014;original draft, reviewing, and editing; C. L. Smith: data collection, and writing&#x02014;reviewing and editing; I. Geornaras: data collection and writing&#x02014;reviewing and editing; J. A. Scanga: conceptualization, funding acquisition, and data collection; K. E. Belk: writing&#x02014;reviewing and editing; R. J. Delmore: data collection and writing&#x02014;reviewing and editing; D. L. Clark: conceptualization, funding acquisition, data collection, and writing&#x02014;reviewing and editing; and M. N. 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