<?xml version="1.0" encoding="UTF-8"?><article article-type="research-article" dtd-version="2.3" xml:lang="EN" annotation-version="1.0"><front><journal-meta><journal-id journal-id-type="hwp">mmb</journal-id><journal-id journal-id-type="publisher-id">mmb</journal-id><journal-title>Meat and Muscle Biology</journal-title><abbrev-journal-title abbrev-type="full">MMB</abbrev-journal-title><issn pub-type="epub">2575-985X</issn><publisher><publisher-name>American Meat Science Association</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">2018-03-0004</article-id><article-id pub-id-type="doi">10.22175/mmb2018.03.0004</article-id><article-categories><subj-group subj-group-type="heading"><subject/></subj-group></article-categories><title-group><article-title>Honduran and U.S. Consumer Assessment of Beef from Various Production Systems with or Without Marinating</article-title></title-group><contrib-group><contrib contrib-type="author" contact-id="0" contact-type="auto"><name><surname>Gomez</surname><given-names>A. R.</given-names></name><aff><label>1</label>Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, 79409, USA</aff></contrib><contrib contrib-type="author" corresp="yes" contact-id="0" contact-type="auto"><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><aff><label>1</label>Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, 79409, USA</aff></contrib><contrib contrib-type="author" contact-id="8990140399033745331" contact-type="auto"><name><surname>O’Quinn</surname><given-names>T. G.</given-names></name><aff><label>2</label>Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS, 66506 USA</aff></contrib><contrib contrib-type="author" contact-id="9446779953340164225" contact-type="auto"><name><surname>Bueso</surname><given-names>M. E.</given-names></name><aff><label>1</label>Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, 79409, USA</aff></contrib><contrib contrib-type="author" contact-id="14844430523273889023" contact-type="auto"><name><surname>Brooks</surname><given-names>J. C.</given-names></name><aff><label>1</label>Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, 79409, USA</aff></contrib><contrib contrib-type="author" contact-id="1296035091196069718" contact-type="auto"><name><surname>Brashears</surname><given-names>M. M.</given-names></name><aff><label>1</label>Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, 79409, USA</aff></contrib><contrib contrib-type="author" contact-id="11928184478183999859" contact-type="auto"><name><surname>Miller</surname><given-names>M. F.</given-names></name><aff><label>1</label>Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX, 79409, USA</aff></contrib></contrib-group><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Email: <email>andrea.garmyn@ttu.edu</email> (A. J. Garmyn)</corresp></author-notes><pub-date pub-type="ppub"><month>08</month><year>2018</year></pub-date><volume>2</volume><issue>1</issue><fpage>242</fpage><lpage>253</lpage><history><date date-type="received"><day>30</day><month>03</month><year>2018</year></date><date date-type="accepted"><day>17</day><month>07</month><year>2018</year></date></history><permissions><copyright-year>2018</copyright-year><copyright-holder>American Meat Science Association</copyright-holder><license license-type="open-access"><p>This is an open access article distributed under the CC BY-NC-ND license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-nd/4.0/" xmlns:xlink="http://www.w3.org/1999/xlink">http://creativecommons.org/licenses/by-nc-nd/4.0/</ext-link>)</p></license></permissions><abstract><p>Our objective was to evaluate the effects of different Honduran cattle production systems, enhancement, and country of origin on palatability of the <italic>longissimus</italic> muscle aged 21 d postmortem as determined by U.S. and Honduran consumers (<italic>n</italic> = 240/country). U.S.-sourced strip loins (<italic>n</italic> = 10/treatment) were selected: USDA Select (SE) and Top (upper 2/3) Choice (TC). Honduran-sourced strip loins (<italic>n</italic> = 10/treatment) included: 1) dual-purpose bulls, raised on native pasture (HDP), 2) F1 crossbred Brahman bulls finished on a corn-based grain diet for 180 d (HCF), and 3) purebred Brahman bulls finished on a sugarcane-based diet for 180 d (HSC). Ten additional strip loins from each Honduran treatment were selected and enhanced (E; 112% ± 3.5%) with water, salt, and tripolyphosphate, resulting in EHDP, EHCF, and EHSC. Steaks were cooked to 77°C prior to consumer evaluation of tenderness, juiciness, and flavor and overall liking, with classification of each trait as acceptable or unacceptable. Consumers indicated if they were willing to pay 0, 3, 6, or 10 USD/0.45 kg. Consumer data were analyzed using the GLIMMIX procedure of SAS as a split plot design, with treatment as the whole plot factor and country and the country × treatment interaction as the subplot factors, including panel as a random effect. The EHCF had greater (<italic>P</italic> &lt; 0.05) scores for tenderness, juiciness, flavor and overall liking. No differences (<italic>P</italic> &gt; 0.05) were found between TC and SE when scoring palatability traits, but more (<italic>P</italic> &lt; 0.05) consumers found TC acceptable for juiciness compared to SE. Honduran consumers ranked all palatability traits greater than U.S consumers and found a greater percentage of samples acceptable for tenderness (<italic>P</italic> &lt; 0.05). Enhancement of Honduran treatments had a positive effect on palatability traits, as well as the acceptability of those traits. Regardless of the differences in breeds, using high-energy diets and enhancement resulted in greater palatability scores.</p></abstract><kwd-group><title>Keywords: </title><kwd>beef</kwd><kwd>consumers</kwd><kwd>enhancement</kwd><kwd>Honduras</kwd><kwd>palatability</kwd></kwd-group></article-meta><custom-meta-wrap><custom-meta><meta-name>author</meta-name><meta-value>Gomez A. R.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Garmyn A. J.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>O’Quinn T. G.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Bueso M. E.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Brooks J. C.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Brashears M. M.</meta-value></custom-meta><custom-meta><meta-name>author</meta-name><meta-value>Miller M. F.</meta-value></custom-meta></custom-meta-wrap><ar:concepts xmlns:ar="http://appliedrelevance.com"><ar:concept><ar:id>16d2b386b2034b9488996466aaae0b57</ar:id><ar:name>History</ar:name><ar:path a="a">Agronomy|Training &amp; Education|History</ar:path><ar:taxonomy>Agronomy</ar:taxonomy></ar:concept><ar:concept><ar:id>7e1e3f377648773863a504d18b367ea3</ar:id><ar:name>Economics</ar:name><ar:path a="a">Agronomy|Training &amp; Education|Economics</ar:path><ar:taxonomy>Agronomy</ar:taxonomy></ar:concept><ar:concept><ar:id>6260c82d505c39199a09ae0fdfdb00c3</ar:id><ar:name>Soybean</ar:name><ar:path a="a">Crops|Crop Type|Oilseed crops|Soybean</ar:path><ar:taxonomy>Crops</ar:taxonomy></ar:concept><ar:concept><ar:id>ed3d69e56a1a51214c0b93418da7e7c6</ar:id><ar:name>Composition</ar:name><ar:path a="a">Crops|Plant Improvement|Traits|Composition</ar:path><ar:taxonomy>Crops</ar:taxonomy></ar:concept><ar:concept><ar:id>3266524df2419aad3a32ade779d3b586</ar:id><ar:name>Protein</ar:name><ar:path a="a">Crops|Plant Improvement|Traits|Nutritive value|Protein</ar:path><ar:taxonomy>Crops</ar:taxonomy></ar:concept><ar:concept><ar:id>29bf7a323f1088692c1ad29f08f4f573</ar:id><ar:name>Si</ar:name><ar:path a="a">Soils|Miscellaneous|Si</ar:path><ar:taxonomy>Soils</ar:taxonomy></ar:concept><ar:concept><ar:id>86ffbce6771a11bbf5a1ff1b779463c8</ar:id><ar:name>agric</ar:name><ar:path a="a">Soils|Miscellaneous|agric</ar:path><ar:taxonomy>Soils</ar:taxonomy></ar:concept><ar:concept><ar:id>58412bbc1e3f44b73e9be60f47ed3857</ar:id><ar:name>thicknesses</ar:name><ar:path a="a">Soils|Miscellaneous|thicknesses</ar:path><ar:taxonomy>Soils</ar:taxonomy></ar:concept></ar:concepts></front><body><sec sec-type="introduction"><title>Introduction</title><p>The United States is the largest producer of high quality beef in the world, with most cattle fed a high-concentrate grain-based finishing diet. Moreover, according to United States Department of Agriculture (<xref ref-type="bibr" rid="r55">USDA, 2015</xref>), the U.S. is the fourth largest beef exporting country in the world and the largest importer of beef. The U.S. has increased its share of imported meat from countries with alternative beef production practices, such as Central and South American countries (<xref ref-type="bibr" rid="r54">USDA, 2012</xref>). Central and South American beef production, however, is mainly based on a grazing system utilizing <italic>Bos indicus</italic> cattle.</p><p>Previous studies have found differences in carcass composition from grain finished and grass finished cattle (<xref ref-type="bibr" rid="r42">Riley et al., 2005</xref>; <xref ref-type="bibr" rid="r56">Van Elswyk and McNeill, 2014</xref>). Additionally, fat deposition varies depending on breed or biological cattle type with <italic>Bos taurus</italic> having a higher fat deposition than <italic>Bos indicus</italic> (<xref ref-type="bibr" rid="r10">Crouse et al., 1989</xref>; <xref ref-type="bibr" rid="r59">Warren et al., 2008</xref>). Previous studies suggest breed variance can increase intramuscular fat to improve meat palatability traits (<xref ref-type="bibr" rid="r32">Muir et al., 1998</xref>; <xref ref-type="bibr" rid="r44">Sadkowski et al., 2014</xref>), ultimately affecting meat quality and acceptability with the consumer (<xref ref-type="bibr" rid="r22">Koch et al., 1976</xref>; <xref ref-type="bibr" rid="r23">Koch et al., 1979</xref>; <xref ref-type="bibr" rid="r28">Maughan et al., 2012</xref>).</p><p>Crossbreeding has been implemented, especially in tropical and subtropical areas, to achieve heterosis, thereby enhancing cattle’s genetics and contributing to more efficient meat production. In addition to crossbreeding, Latin American countries have been implementing the incorporation of feed supplements to improve growth performance or meat quality. In particular, corn has been added to the diets as an alternative to accelerate cattle fattening.</p><p>Additionally, as the world population increases, there is an increase in food demand, especially in developing countries. In 2015, the Food and Agriculture Organization reported 1 million undernourished people living in Honduras (<xref ref-type="bibr" rid="r15">FAO, 2015</xref>). To improve population nutritional conditions, more efficient production systems and alternatives for improving animal protein access are needed. Due to the success of U.S. beef in international markets, some countries have tried to adapt their production systems to produce beef similar in quality and composition to U.S. beef. Currently, however, there are no data describing consumer (Honduran or U.S.) perception of grain-finished U.S. beef compared to Honduran beef derived from these various alternative cattle finishing systems.</p><p>To overcome problems with consumer acceptability of low quality meat, enhancement of beef products with the addition of brines containing water, phosphates and salt has also been used in the industry. It has been demonstrated that the application of enhancement results in improved palatability and greater yields (<xref ref-type="bibr" rid="r26">Lee et al., 2014</xref>; <xref ref-type="bibr" rid="r46">Scanga et al., 2000</xref>; <xref ref-type="bibr" rid="r47">Sheard and Tali, 2004</xref>).</p><p>The objective of this study was to characterize the eating quality of beef from the United States compared to non-enhanced and enhanced Honduran beef from cattle raised using alternative finishing practices according to both Honduran and U.S. consumers to compare consumer preferences between these 2 countries.</p></sec><sec sec-type="materials|methods"><title>Materials and Methods</title><sec><title>Product collection</title><p>Eight total treatments were utilized for this study including 2 U.S. treatments and 6 Honduran treatments. U.S. treatments included USDA Select strip loins (SE; <italic>n</italic> = 10) and USDA Top (upper 2/3) Choice strip loins (TC; <italic>n</italic> = 10). The U.S.-sourced strip loins (IMPS #180; <xref ref-type="bibr" rid="r33">NAMP, 2011</xref>) were collected at a federally inspected commercial beef processing facility by trained Texas Tech personnel. Carcasses were chilled for approximately 24 h before grading. Carcass fabrication occurred between 24 and 32 h postmortem. Strip loins were transported to the Gordon. W. Davis Meat Science Laboratory located in Lubbock, Texas. Honduran treatments were collected at Agroindustrias Del Corral located in Siguatepeque, Honduras. All experimental procedures with Honduran animals were conducted in accordance with a Texas Tech University Animal Care and Use Committee protocol (Protocol # 14071–09). Strip loins collected in Honduras included 3 different treatments, consisting of 1) predominantly Brahman, Holstein, and Brown Swiss crossbred (information about the exact breed composition was unknown), dual-purpose cattle [HDP (milk and beef production); <italic>n</italic> = 10], that were raised solely on native pasture (<italic>Hyparrhenia rufa</italic>) and improved grasses (<italic>Panicum maximum, Cynodon plectostachyus, Digitaria swazilandensis, Brachiaria decumbens,</italic> and <italic>Brachiaria brizantha</italic>) resulting in an age at slaughter of approximately 40 mo; 2) cattle finished on a high energy diet consisting of cracked corn, palm kernel, and sugarcane for 180 d (HCF; <italic>n</italic> = 10). Cattle used for HCF treatment were F1 crosses between Brahman and either Senepol, Red Angus, or Simmental that averaged 24 mo at slaughter. A third treatment group consisted of cattle finished on a by-product-based diet consisting of sugarcane, palm kernel meal, poultry litter, and cracked corn for 180 d, (HSC; <italic>n</italic> = 10) but were predominantly native purebred Brahman that were approximately 24 mo of age at slaughter. All Honduran cattle involved were bulls, as it is customary to leave male cattle intact during growing and finishing in Honduras. Ingredient composition for HCF and HSC can be found in <xref ref-type="table" rid="tbl1">Table 1</xref>. Diets were formulated to provide approximately 13.5% crude protein on a DM basis, but feed composition was not tested. Carcass fabrication occurred between 18 and 24 h postmortem. Ten additional paired strip loins from each of the 3 Honduran sourced treatments were collected from the other side of the carcass and enhanced at 24 h postmortem at a 112% (± 3.5%) pump rate using a multi needle injector (Accujector 450, GEA Group; Bogotá D. C., Colombia) with a target concentration of 0.25% tripolyphosphate and 0.50% salt in the final product. Enhancement resulted in the following three treatments: enhanced HDP (EHDP), enhanced HCF (EHCF), and enhanced HSC (EHSC).</p><table-wrap id="tbl1" position="float"><label>Table 1.</label><caption><p>Ingredient composition (DM basis) of the experimental diets fed in finishing diets to Honduran cattle</p></caption><table frame="hsides" rules="groups"><thead><tr><td rowspan="2">Item</td><td colspan="2" align="center">Treatment<hr/></td></tr><tr><td align="center">HCF<sup>1</sup></td><td align="center">HSC<sup>1</sup></td></tr></thead><tbody><tr><td colspan="4">Ingredient, %</td></tr><tr><td> Fresh Sugar Cane</td><td align="char" char=".">15.00</td><td align="char" char=".">37.30</td></tr><tr><td> Palm Kernel Meal</td><td align="char" char=".">20.00</td><td align="char" char=".">20.40</td></tr><tr><td> Poultry Litter, dry</td><td align="char" char=".">8.00</td><td align="char" char=".">19.90</td></tr><tr><td> Soybean Meal</td><td align="char" char=".">5.00</td><td align="center">--</td></tr><tr><td> Cracked Corn</td><td align="char" char=".">46.00</td><td align="char" char=".">15.80</td></tr><tr><td> Molasses</td><td align="char" char=".">5.00</td><td align="char" char=".">6.5</td></tr><tr><td> Calcium Carbonate</td><td align="char" char=".">1.00</td><td align="center">--</td></tr></tbody></table><table-wrap-foot><fn><p><sup>1</sup>HCF – Honduran corn-fed, HSC – Honduran sugar cane.</p></fn></table-wrap-foot></table-wrap></sec><sec><title>Sample preparation</title><p>Strip loins from both countries were aged for 21 d at 0 to 4°C under vacuum. Eight 2.5-cm steaks were fabricated from each strip loin starting at the anterior end using a manual meat slicer (Model X13E, 33 cm; ITW Food Equipment Group LLC, Louisville, KY) to ensure uniform thickness. The anterior-most steak from each strip loin was assigned to proximate analysis, and the next steak was assigned to Warner-Bratzler shear force (WBSF). The next 6 steaks were assigned randomly to consumer sensory panel evaluation. Consecutively cut steaks were paired, with 1 steak from each pair assigned to consumer testing in the U.S. and the other steak assigned to consumer testing in Honduras. Steaks were frozen (–20°C) at 21 d post mortem and remained frozen until each evaluation. Any steaks destined to be tested in the country other than where collection took place, including all Honduran steaks destined for shear force and compositional analyses, were shipped via commercial air in a frozen state (0°C), and were stored frozen (–20°C) on arrival until further sample evaluation. Steaks were thawed for 24 h at 2 to 4°C prior to consumer evaluation, shear force analysis and proximate analysis.</p></sec><sec><title>Proximate analysis</title><p>Proximate analysis was conducted to determine chemical values of fat, protein and moisture. Frozen samples were thawed at 2 to 4°C for 24 h prior to analysis. Steaks were trimmed of external fat and additional muscles (<italic>longissimus costarum</italic> and the <italic>multifidus dorsi</italic>). Samples were ground in a commercial food grinder (Krups 150-Watt Meat Grinder item #402–70, Krups, Shelton, CT). Proximate analysis was conducted on approximately 200-g samples using an AOAC-approved (Official Method 2007.04; <xref ref-type="bibr" rid="r3">Anderson, 2007</xref>) near infrared spectrophotometer (FoodScan, FOSS NIRsystems, Inc., Laurel, MD). Chemical values (%) of fat, protein, and moisture were determined.</p></sec><sec><title>Warner-Bratzler Shear Force analysis</title><p>Tenderness was determined using a WBSF analyzer (G-R Elec. Mfg., Manhattan, KS). . Frozen steaks were thawed at 2 to 4°C for 24 h prior to analysis. All steaks were cooked on non-stick clamshell grills (George Foreman, Wilkes Barre, PA) to an internal temperature of 77°C and then cooled overnight at 2°C for 24 h. Six 1.3-cm core samples were removed parallel to the orientation of the muscle fibers, from each steak and sheared once, perpendicular to the orientation of the muscle fibers. The 6 values were averaged to determine 1 shear force value (kg) for each steak sample.</p></sec><sec><title>Consumer sensory evaluation</title><p>The Texas Tech University Institutional Review Board approved procedures for use of human subjects for consumer panel evaluation of sensory attributes in the U.S. and Honduras. Consumer sensory evaluation was based on the American Meat Science Association’s sensory guidelines (<xref ref-type="bibr" rid="r2">American Meat Science Association, 2015</xref>). Strip loin samples were thawed at 2 to 4°C for 24 h prior to cooking for consumer evaluation. Steaks were cooked on non-stick clamshell grills (George Foreman, Wilkes Barre, PA) to an internal temperature 77°C, monitored using a thermocouple probe (Type J; Cole-Parmer Instrument Company, Vernon Hills, IL) attached to a thermometer (Digi-sense; Cole Parmer). Each steak was then portioned into 8 uniform pieces. Consumer panelists (<italic>n</italic> = 240) in the U.S. were recruited in Lubbock, Texas; consumer panelists (<italic>n</italic> = 240) in Honduras were recruited either at Zamorano University in Tegucigalpa, Honduras or Siguatepeque, Honduras. U.S. consumer panels were conducted in the Animal and Food Sciences building at Texas Tech University. Panelists were seated individually in numbered booths and were each provided with a ballot, toothpick, napkin, plastic utensils, cup of water, and unsalted crackers (used as palate cleanser). Honduran consumer panels were conducted at an outdoor booth at the annual Pan- American Celebration Fair and at a supermarket in Siguatepeque, Honduras. Consumers were seated at open tables in Honduras, but were instructed not to communicate with each other during assessment and were provided the same supplies as U.S. consumers. Each ballot included an information sheet about the project for the consumer, demographic survey, and 8 sample evaluation ballots. Verbal instructions were given to consumers prior to each panel regarding the ballot, the procedure to follow for the panel, and the use of palate cleansers. Ballots for Honduran consumer panels were translated and provided in Spanish.</p><p>Eight samples were served to each consumer in a predetermined order. All samples were identified with a unique 4-digit identification number. An 8-point hedonic scale was used to rate juiciness, flavor liking, tenderness, and overall liking. On the scale, tenderness (1 = extremely tough, 2 = very tough, 3 = moderately tough, 4 = slightly tough, 5 = slightly tender, 6 = moderately tender, 7 = very tender, 8 = extremely tender), juiciness (1 = extremely dry, 2 = very dry, 3 = moderately dry, 4 = slightly dry, 5 = slightly juicy, 6 = moderately juicy, 7 = very juicy, 8 = extremely juicy), and flavor and overall liking (1 = extremely dislike, 2 = very much dislike, 3 = moderately dislike, 4 = slightly dislike, 5 = slightly like, 6 = moderately like, 7 = very much like, 8 = extremely like) were evaluated. Consumers were also asked to indicate whether the tenderness, juiciness, flavor, and overall eating quality of the sample were acceptable (Si) or unacceptable (No). Additionally, panelists indicated how much they were willing to pay for each sample by selecting 1 of 4 monetary values (U. S. consumers selected either $0, 3, 6, or 10 per 0.45 kg). The national Honduran currency (Lempiras; L.) was used in Honduras so that consumers selected L. 0, 60, 120, or 200 per 0.45 kg. At the time of testing, one U.S. dollar was equivalent to L. 20.00.</p></sec><sec><title>Statistical methods</title><p>Statistical analysis was conducted in SAS (Version 9.4; SAS Inst. Inc., Cary, NC). Proximate and shear force data were analyzed using the GLIMMIX procedure as a completely randomized design, with a fixed effect of treatment. The model for consumer rating data was analyzed as a split plot, with treatment as the whole plot factor and country and the country × treatment interaction as the subplot factors. Consumer acceptability data and willingness to pay data were analyzed using a model with a binomial error distribution. For all consumer data, panel was included as a random effect. Treatment least square means were separated with the PDIFF option of SAS at a significance level of <italic>P</italic> &lt; 0.05. For all analyses, denominator degrees of freedom were calculated using the Kenward-Roger approximation.</p></sec></sec><sec sec-type="results|discussion"><title>Results and Discussion</title><sec><title>Proximate analysis</title><p>Proximate composition results are presented in <xref ref-type="table" rid="tbl2">Table 2</xref>. Treatment had an effect (<italic>P</italic> &lt; 0.01) on fat, protein and moisture. Fat percentages ranged widely among treatments (1.63 to 9.24%). Fat percentage of TC was greater (<italic>P</italic> &lt; 0.05) than all other treatments, and consequently moisture percentage was the lowest (<italic>P</italic> &lt; 0.05) for this treatment. Select had greater (<italic>P</italic> &lt; 0.05) fat than both the enhanced and non-enhanced HDP and HSC treatments, but did not differ (<italic>P</italic> &gt; 0.05) from either HCF treatment. Fat percentages of U.S. samples in this study were slightly higher than previously published values for top loin steaks of their respective quality grades (<xref ref-type="bibr" rid="r9">Corbin et al., 2015</xref>; <xref ref-type="bibr" rid="r14">Emerson et al., 2013</xref>; <xref ref-type="bibr" rid="r19">Hunt et al., 2014</xref>). However, fat percentages were lower in our study than previously reported values of Honduran grass fed (5.78%) and Honduran grain fed (6.47%) top loin steaks (<xref ref-type="bibr" rid="r6">Bueso, 2015</xref>). The difference in breed composition between North and Central American cattle as well as differences in diet likely contributed to the elevated fat percentage of U.S. samples. Moisture content was similar (<italic>P</italic> &gt; 0.05) in all three enhanced treatments as well as HDP, and was greater (<italic>P</italic> &lt; 0.05) compared to HCF, SE, and TC. Moisture content increases with addition of moisture enhancement in meat products (<xref ref-type="bibr" rid="r50">Stetzer et al., 2008</xref>). Moreover, these results agree with other studies reporting an inverse relationship between moisture and fat (<xref ref-type="bibr" rid="r11">Delgado et al., 2005</xref>; <xref ref-type="bibr" rid="r48">Smith et al., 2011</xref>). There were expected differences in moisture and fat content; however, a 5% range in protein composition was also observed. Protein was greatest (<italic>P</italic> &lt; 0.05) in SE and lowest in EHCF.</p><table-wrap id="tbl2" position="float"><label>Table 2.</label><caption><p>Least squares means of proximate analysis for chemical fat, protein, and moisture and Warner-Bratzler shear force values</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Treatment</td><td align="center">Fat, %</td><td align="center">Protein, %</td><td align="center">Moisture, %</td><td align="center">WBSF, kg</td></tr></thead><tbody><tr><td>EHDP<sup>1</sup></td><td align="char" char=".">1.63<sup>de</sup></td><td align="char" char=".">21.51<sup>c</sup></td><td align="char" char=".">75.53<sup>a</sup></td><td align="char" char=".">4.81<sup>ab</sup></td></tr><tr><td>EHCF<sup>2</sup></td><td align="char" char=".">2.81<sup>bcd</sup></td><td align="char" char=".">19.35<sup>e</sup></td><td align="char" char=".">74.59<sup>ab</sup></td><td align="char" char=".">2.50<sup>e</sup></td></tr><tr><td>EHSC<sup>3</sup></td><td align="char" char=".">2.37<sup>cde</sup></td><td align="char" char=".">20.51<sup>d</sup></td><td align="char" char=".">74.94<sup>ab</sup></td><td align="char" char=".">4.18<sup>bc</sup></td></tr><tr><td>HDP<sup>4</sup></td><td align="char" char=".">1.53<sup>e</sup></td><td align="char" char=".">22.64<sup>b</sup></td><td align="char" char=".">74.92<sup>ab</sup></td><td align="char" char=".">5.65<sup>a</sup></td></tr><tr><td>HCF<sup>5</sup></td><td align="char" char=".">3.51<sup>bc</sup></td><td align="char" char=".">22.62<sup>b</sup></td><td align="char" char=".">72.06<sup>c</sup></td><td align="char" char=".">3.74<sup>cd</sup></td></tr><tr><td>HSC<sup>6</sup></td><td align="char" char=".">2.59<sup>cde</sup></td><td align="char" char=".">22.08<sup>bc</sup></td><td align="char" char=".">73.73<sup>b</sup></td><td align="char" char=".">5.19<sup>ab</sup></td></tr><tr><td>SE<sup>7</sup></td><td align="char" char=".">4.00<sup>b</sup></td><td align="char" char=".">24.28<sup>a</sup></td><td align="char" char=".">71.14<sup>c</sup></td><td align="char" char=".">2.72<sup>de</sup></td></tr><tr><td>TC<sup>8</sup></td><td align="char" char=".">9.24<sup>a</sup></td><td align="char" char=".">22.51<sup>b</sup></td><td align="char" char=".">67.41<sup>d</sup></td><td align="char" char=".">2.69<sup>e</sup></td></tr><tr><td>SEM<sup>9</sup></td><td align="char" char=".">0.47</td><td align="char" char=".">0.32</td><td align="char" char=".">0.47</td><td align="char" char=".">0.44</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a-e</sup>Least square means in the same column with different superscripts differ, <italic>P</italic> &lt; 0.05.</p></fn><fn><p><sup>1</sup>EHDP = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran dual purpose.</p></fn><fn><p><sup>2</sup>EHCF = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran corn-fed diet.</p></fn><fn><p><sup>3</sup>EHSC = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran sugar cane diet.</p></fn><fn><p><sup>4</sup>HDP = Honduran dual purpose.</p></fn><fn><p><sup>5</sup>HCF = Honduran corn-fed diet.</p></fn><fn><p><sup>6</sup>HSC = Honduran sugar cane diet.</p></fn><fn><p><sup>7</sup>SE = USDA Select.</p></fn><fn><p><sup>8</sup>TC = USDA top (upper 2/3) Choice.</p></fn><fn><p><sup>9</sup> SEM (largest) of the least square means.</p></fn></table-wrap-foot></table-wrap></sec><sec><title>Warner-Bratzler Shear Force</title><p>As seen in <xref ref-type="table" rid="tbl2">Table 2</xref>, treatment influenced (<italic>P</italic> &lt; 0.01) shear force values. HDP had greater (<italic>P</italic> &lt; 0.05) WBSF values when compared to all other treatments except EHDP and HSC. The 2 US treatments along with EHCF had lower WBSF values than all other Honduran treatments, regardless of enhancement (<italic>P</italic> &lt; 0.05), except that SE and HCF were similar (<italic>P</italic> &gt; 0.05). Enhancement did not statistically reduce WBSF for HDP or HSC when compared to their non-enhanced counterparts even though there was a mean difference of 0.8 kg and 1.0 kg, respectively. However, previous research reports that consumers have been able to detect differences in WBSF of this magnitude (0.8 to 1.0 kg) when evaluating tenderness (<xref ref-type="bibr" rid="r31">Miller et al., 1995</xref>). Lower WBSF values in HCF when compared to HDP could have been a result of breed differences between these treatments, as well as a dietary effect, and/or variable age at slaughter. Tenderness in <italic>Bos indicus</italic> cattle can be of concern, especially as the percentage of <italic>Bos indicus</italic> increases (<xref ref-type="bibr" rid="r10">Crouse et al., 1989</xref>). High shear force values of steaks from <italic>Bos indicus</italic> cattle can be influenced by the elevated calpastatin levels (<xref ref-type="bibr" rid="r60">Whipple et al., 1990</xref>) and less intramuscular fat (<xref ref-type="bibr" rid="r17">Highfill et al., 2012</xref>). Additionally, greater WBSF values for HDP could have been affected by the fact that the animals used in this study were killed at an older age (typically 40 mo), potentially influencing collagen solubility (<xref ref-type="bibr" rid="r42">Riley et al., 2005</xref>). Previous studies have demonstrated a correlation between connective tissue, collagen solubility, and meat tenderness (<xref ref-type="bibr" rid="r7">Campo et al., 2000</xref>) and an effect of age on connective tissue (<xref ref-type="bibr" rid="r1">Allingham et al., 1998</xref>). As the age of animals increases, tenderness decreases (<xref ref-type="bibr" rid="r18">Hiner and Hankins, 1950</xref>). Evidence of the effect of calpastatin and collagen on tenderness when Brahman is included in the breeding is presented by <xref ref-type="bibr" rid="r42">Riley et al. (2005)</xref>. Additionally, <xref ref-type="bibr" rid="r10">Crouse et al. (1989)</xref> found that with an increase in <italic>Bos indicus</italic> inheritance, an increase in shear force values was observed. Previous studies have demonstrated that enhancement lowers shear force values (<xref ref-type="bibr" rid="r61">Wicklund et al., 2006</xref>). In addition, differences in shear force values between USDA quality grades have been reported in previous research (<xref ref-type="bibr" rid="r14">Emerson et al., 2013</xref>; <xref ref-type="bibr" rid="r16">Garmyn et al., 2011</xref>; <xref ref-type="bibr" rid="r19">Hunt et al., 2014</xref>) even though no difference in WBSF between SE and TC was observed in our study.</p></sec><sec><title>Consumer demographics</title><p>Demographic profiles of consumers fed in Lubbock, Texas are shown in <xref ref-type="table" rid="tbl3">Table 3</xref>. More females participated in this study than males. The ages of the consumers were distributed from &lt; 20 to &gt; 60 yr old, with the 20 to 29 age group having the largest number of participants, followed by 30 to 39 and &gt; 60 groups. Most of the consumers were from the U.S., with Canada, Mexico, and any other country of origin each contributing &lt; 0.5% each. Most consumers were employed as a tradesperson, in administration, or a laborer, rather than being a student or unemployed.</p><table-wrap id="tbl3" position="float"><label>Table 3.</label><caption><p>Demographic characteristics of consumers (n = 240) who participated in U.S. consumer sensory panels</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Characteristic</td><td align="center">Response</td><td align="center">Consumers, %</td></tr></thead><tbody><tr><td>Gender</td><td align="center">Male</td><td align="char" char=".">42.9</td></tr><tr><td/><td align="center">Female</td><td align="char" char=".">57.1</td></tr><tr><td>Age</td><td align="center">&lt;20</td><td align="char" char=".">8.3</td></tr><tr><td/><td align="center">20-29</td><td align="char" char=".">31.4</td></tr><tr><td/><td align="center">30-39</td><td align="char" char=".">19.7</td></tr><tr><td/><td align="center">40-49</td><td align="char" char=".">12.7</td></tr><tr><td/><td align="center">50-59</td><td align="char" char=".">8.3</td></tr><tr><td/><td align="center">&gt;60</td><td align="char" char=".">19.7</td></tr><tr><td>Occupation</td><td align="center">Student</td><td align="char" char=".">27.9</td></tr><tr><td/><td align="center">Not currently employed</td><td align="char" char=".">11.7</td></tr><tr><td/><td align="center">Other (tradesperson, administration, ales, laborer)</td><td align="char" char=".">60.4</td></tr><tr><td>Country of Origin</td><td align="center">United States of America</td><td align="char" char=".">98.8</td></tr><tr><td/><td align="center">Canada</td><td align="char" char=".">0.4</td></tr><tr><td/><td align="center">Mexico</td><td align="char" char=".">0.4</td></tr><tr><td/><td align="center">Other</td><td align="char" char=".">0.4</td></tr></tbody></table></table-wrap><p>Honduran consumers were recruited from different cities around Honduras in the Pan-American Annual Fair at Zamorano University located in Tegucigalpa and at a supermarket located in Siguatepeque, Honduras. As seen in <xref ref-type="table" rid="tbl4">Table 4</xref>, most consumers were males, with nearly two-thirds of the participants representing college aged people under the age of 30 yr old. Much like the U.S. consumers, however, the highest percentage of consumers stated their occupation fell within the “other”, category including tradesperson, administration, sales, laborer, etc. Most consumers were from Honduras, followed by Ecuador, but at least 6 other Central/South American countries were represented.</p><table-wrap id="tbl4" position="float"><label>Table 4.</label><caption><p>Demographic characteristics of consumers (n = 240) who participated in Honduran consumer sensory panels</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Characteristic</td><td align="center">Response</td><td align="center">Consumers, %</td></tr></thead><tbody><tr><td>Gender</td><td align="center">Male</td><td align="char" char=".">54.5</td></tr><tr><td/><td align="center">Female</td><td align="char" char=".">45.5</td></tr><tr><td>Age</td><td align="center">&lt;20</td><td align="char" char=".">15.8</td></tr><tr><td/><td align="center">20-29</td><td align="char" char=".">49.1</td></tr><tr><td/><td align="center">30-39</td><td align="char" char=".">18.4</td></tr><tr><td/><td align="center">40-49</td><td align="char" char=".">10.7</td></tr><tr><td/><td align="center">50-59</td><td align="char" char=".">3.0</td></tr><tr><td/><td align="center">&gt;60</td><td align="char" char=".">3.0</td></tr><tr><td>Occupation</td><td align="center">Student</td><td align="char" char=".">32.90</td></tr><tr><td/><td align="center">Not employed</td><td align="char" char=".">1.28</td></tr><tr><td/><td align="center">Other</td><td align="char" char=".">65.81</td></tr><tr><td>Country of Origin</td><td align="center">Honduras</td><td align="char" char=".">80.85</td></tr><tr><td/><td align="center">Guatemala</td><td align="char" char=".">2.12</td></tr><tr><td/><td align="center">Nicaragua</td><td align="char" char=".">1.70</td></tr><tr><td/><td align="center">El Salvador</td><td align="char" char=".">0.85</td></tr><tr><td/><td align="center">Panama</td><td align="char" char=".">1.27</td></tr><tr><td/><td align="center">Colombia</td><td align="char" char=".">1.70</td></tr><tr><td/><td align="center">Ecuador</td><td align="char" char=".">6.38</td></tr><tr><td/><td align="center">Other</td><td align="char" char=".">5.10</td></tr></tbody></table></table-wrap></sec><sec><title>Consumer tenderness</title><p>As seen in <xref ref-type="table" rid="tbl5">Table 5</xref>, treatment and country where product was evaluated both influenced (<italic>P</italic> &lt; 0.01) consumer ratings for tenderness, but were not interactive (<italic>P</italic> = 0.23). Tenderness acceptability followed a similar trend as no 2-way interaction was observed (<italic>P</italic> = 0.96; <xref ref-type="table" rid="tbl6">Table 6</xref>), while treatment (<xref ref-type="table" rid="tbl7">Table 7</xref>) influenced (<italic>P</italic> &lt; 0.01) the proportion of samples considered acceptable for tenderness as did country where product was evaluated (<italic>P</italic> &lt; 0.01). Honduran consumers rated the samples more tender (<italic>P</italic> &lt; 0.05) than U.S. consumers, regardless of treatment. Consequently, a generally greater percentage of Honduran consumers found the samples acceptable for tenderness than the U.S. consumers (<xref ref-type="table" rid="tbl6">Table 6</xref>). Regardless of country where product was evaluated, consumers rated EHCF samples most tender (<italic>P</italic> &lt; 0.05) followed by similar (<italic>P</italic> &gt; 0.05) scores for TC and SE (<xref ref-type="table" rid="tbl5">Table 5</xref>). Results of the current study demonstrate the positive effect of enhancement on tenderness scores (<xref ref-type="table" rid="tbl5">Table 5</xref>) and tenderness acceptability (<xref ref-type="table" rid="tbl7">Table 7</xref>) when evaluated by consumers. Greater tenderness ratings were observed only for EHCF when compared to HCF. Although numerical increases were seen, no differences (<italic>P</italic> &gt; 0.05) were detected between HDP with EHDP or between HSC with EHSC. Even so, in terms of the percentage of samples rated as acceptable for tenderness, enhancement had a positive effect when comparing all 3 Honduran treatments to their enhanced counterparts.</p><table-wrap id="tbl5" position="float"><label>Table 5.</label><caption><p>Least square means for consumer (n = 480) ratings for each palatability trait by treatment and country where product was evaluated</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Treatment</td><td align="center">Tenderness<sup>1</sup></td><td align="center">Juiciness<sup>2</sup></td><td align="center">Flavor liking<sup>3</sup></td><td align="center">Overall liking<sup>3</sup></td></tr></thead><tbody><tr><td> EHDP<sup>4</sup></td><td align="char" char=".">3.62<sup>ef</sup></td><td align="char" char=".">3.62<sup>f</sup></td><td align="char" char=".">4.02<sup>cd</sup></td><td align="char" char=".">3.89<sup>d</sup></td></tr><tr><td> EHCF<sup>5</sup></td><td align="char" char=".">6.49<sup>a</sup></td><td align="char" char=".">5.82<sup>a</sup></td><td align="char" char=".">6.22<sup>a</sup></td><td align="char" char=".">6.34<sup>a</sup></td></tr><tr><td> EHSC<sup>6</sup></td><td align="char" char=".">4.59<sup>cd</sup></td><td align="char" char=".">4.62<sup>bc</sup></td><td align="char" char=".">4.75<sup>b</sup></td><td align="char" char=".">4.76<sup>bc</sup></td></tr><tr><td> HDP<sup>7</sup></td><td align="char" char=".">2.93<sup>f</sup></td><td align="char" char=".">3.25<sup>f</sup></td><td align="char" char=".">3.28<sup>e</sup></td><td align="char" char=".">3.15<sup>e</sup></td></tr><tr><td> HCF<sup>8</sup></td><td align="char" char=".">4.73<sup>c</sup></td><td align="char" char=".">4.32<sup>cd</sup></td><td align="char" char=".">4.35<sup>bc</sup></td><td align="char" char=".">4.54<sup>c</sup></td></tr><tr><td> HSC<sup>9</sup></td><td align="char" char=".">3.88<sup>de</sup></td><td align="char" char=".">3.75<sup>ef</sup></td><td align="char" char=".">3.66<sup>de</sup></td><td align="char" char=".">3.75<sup>d</sup></td></tr><tr><td> SE<sup>10</sup></td><td align="char" char=".">5.24<sup>bc</sup></td><td align="char" char=".">4.46<sup>bc</sup></td><td align="char" char=".">4.63<sup>b</sup></td><td align="char" char=".">4.85<sup>bc</sup></td></tr><tr><td> TC<sup>11</sup></td><td align="char" char=".">5.62<sup>b</sup></td><td align="char" char=".">4.94<sup>b</sup></td><td align="char" char=".">4.82<sup>b</sup></td><td align="char" char=".">5.17<sup>b</sup></td></tr><tr><td>SEM<sup>12</sup></td><td align="char" char=".">0.26</td><td align="char" char=".">0.19</td><td align="char" char=".">0.19</td><td align="char" char=".">0.21</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td></tr><tr><td colspan="6">Country</td></tr><tr><td> U.S.</td><td align="char" char=".">4.39<sup>b</sup></td><td align="char" char=".">4.10<sup>b</sup></td><td align="char" char=".">4.26<sup>b</sup></td><td align="char" char=".">4.22<sup>b</sup></td></tr><tr><td> Honduras</td><td align="char" char=".">4.88<sup>a</sup></td><td align="char" char=".">4.66<sup>a</sup></td><td align="char" char=".">4.67<sup>a</sup></td><td align="char" char=".">4.90<sup>a</sup></td></tr><tr><td>SEM</td><td align="char" char=".">0.10</td><td align="char" char=".">0.07</td><td align="char" char=".">0.07</td><td align="char" char=".">0.08</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td></tr><tr><td><italic>P</italic>-value<sup>13</sup></td><td align="char" char=".">0.23</td><td align="char" char=".">0.09</td><td align="char" char=".">0.15</td><td align="char" char=".">0.42</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a-f</sup>Least square means in the same column with different superscripts differ, <italic>P</italic> &lt; 0.05.</p></fn><fn><p><sup>1</sup>Tenderness: 1 = extremely tough, 2 = very tough, 3 = moderately tough, 4 = slightly tough, 5 = slightly tough, 6 = moderately tender, 7 = very tender, 8 = extremely tender.</p></fn><fn><p><sup>2</sup>Juiciness: 1 = extremely dry, 2 = very dry, 3 = moderately dry, 4 = slightly dry, 5 = slightly juicy, 6 = moderately juicy, 7 = very juicy, 8 = extremely juicy.</p></fn><fn><p><sup>3</sup>Flavor/Overall Liking: 1 = extremely dislike, 2 = very much dislike, 3 = moderately dislike, 4 = slightly dislike, 5 = slightly like, 6 = moderately like, 7 = very much like, 8 = extremely like.</p></fn><fn><p><sup>4</sup>EHDP = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran dual purpose.</p></fn><fn><p><sup>5</sup>EHCF = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran corn-fed diet.</p></fn><fn><p><sup>6</sup>EHSC = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran sugar cane diet.</p></fn><fn><p><sup>7</sup>HDP = Honduran dual purpose.</p></fn><fn><p><sup>8</sup>HCF = Honduran corn-fed diet.</p></fn><fn><p><sup>9</sup>HSC = Honduran sugar cane diet.</p></fn><fn><p><sup>10</sup>SE = USDA Select.</p></fn><fn><p><sup>11</sup>TC = USDA top (upper 2/3) choice.</p></fn><fn><p><sup>12</sup>SEM (largest) of the least square means.</p></fn><fn><p><sup>13</sup><italic>P</italic>-value of treatment x country interaction.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tbl6" position="float"><label>Table 6.</label><caption><p>The effects of country where product was evaluated and treatment on least square means for consumer (n = 480) acceptability for each palatability trait</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Treatment</td><td align="center">Tenderness, %</td><td align="center">Juiciness, %</td><td align="center">Flavor liking, %</td><td align="center">Overall liking, %</td></tr></thead><tbody><tr><td colspan="6">U.S.</td></tr><tr><td> EHDP<sup>1</sup></td><td align="char" char=".">41.71</td><td align="char" char=".">49.74<sup>ef</sup></td><td align="char" char=".">50.93<sup>efg</sup></td><td align="char" char=".">57.56</td></tr><tr><td> EHCF<sup>2</sup></td><td align="char" char=".">96.19</td><td align="char" char=".">85.81<sup>a</sup></td><td align="char" char=".">90.71<sup>a</sup></td><td align="char" char=".">82.62</td></tr><tr><td> EHSC<sup>3</sup></td><td align="char" char=".">67.81</td><td align="char" char=".">67.83<sup>bc</sup></td><td align="char" char=".">67.51<sup>bc</sup></td><td align="char" char=".">61.40</td></tr><tr><td> HDP<sup>4</sup></td><td align="char" char=".">25.02</td><td align="char" char=".">34.80<sup>g</sup></td><td align="char" char=".">36.94<sup>hi</sup></td><td align="char" char=".">37.55</td></tr><tr><td> HCF<sup>5</sup></td><td align="char" char=".">66.64</td><td align="char" char=".">55.36<sup>cde</sup></td><td align="char" char=".">60.24<sup>cde</sup></td><td align="char" char=".">60.96</td></tr><tr><td> HSC<sup>6</sup></td><td align="char" char=".">42.85</td><td align="char" char=".">38.97<sup>fg</sup></td><td align="char" char=".">39.89<sup>ghi</sup></td><td align="char" char=".">45.20</td></tr><tr><td> SE<sup>7</sup></td><td align="char" char=".">75.61</td><td align="char" char=".">60.25<sup>cde</sup></td><td align="char" char=".">65.75<sup>bcd</sup></td><td align="char" char=".">69.40</td></tr><tr><td> TC<sup>8</sup></td><td align="char" char=".">85.34</td><td align="char" char=".">78.31<sup>ab</sup></td><td align="char" char=".">72.97<sup>b</sup></td><td align="char" char=".">70.84</td></tr><tr><td colspan="6">Honduras</td></tr><tr><td> EHDP</td><td align="char" char=".">50.46</td><td align="char" char=".">54.23<sup>de</sup></td><td align="char" char=".">57.34<sup>cdef</sup></td><td align="char" char=".">51.47</td></tr><tr><td> EHCF</td><td align="char" char=".">97.87</td><td align="char" char=".">86.16<sup>a</sup></td><td align="char" char=".">87.38<sup>a</sup></td><td align="char" char=".">89.79</td></tr><tr><td> EHSC</td><td align="char" char=".">72.27</td><td align="char" char=".">67.67<sup>bc</sup></td><td align="char" char=".">68.27<sup>bc</sup></td><td align="char" char=".">70.56</td></tr><tr><td> HDP</td><td align="char" char=".">29.70</td><td align="char" char=".">38.34<sup>fg</sup></td><td align="char" char=".">33.11<sup>i</sup></td><td align="char" char=".">32.42</td></tr><tr><td> HCF</td><td align="char" char=".">74.24</td><td align="char" char=".">56.36<sup>cde</sup></td><td align="char" char=".">53.82<sup>def</sup></td><td align="char" char=".">61.63</td></tr><tr><td> HSC</td><td align="char" char=".">51.22</td><td align="char" char=".">49.18<sup>ef</sup></td><td align="char" char=".">45.23<sup>fgh</sup></td><td align="char" char=".">46.60</td></tr><tr><td> SE</td><td align="char" char=".">81.96</td><td align="char" char=".">59.02<sup>cde</sup></td><td align="char" char=".">60.19<sup>cde</sup></td><td align="char" char=".">69.82</td></tr><tr><td> TC</td><td align="char" char=".">85.72</td><td align="char" char=".">65.24<sup>cd</sup></td><td align="char" char=".">58.20<sup>cde</sup></td><td align="char" char=".">73.25</td></tr><tr><td>SEM<sup>9</sup></td><td align="char" char=".">0.56</td><td align="char" char=".">0.31</td><td align="char" char=".">0.31</td><td align="char" char=".">0.29</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">0.96</td><td align="char" char=".">0.04</td><td align="char" char=".">0.03</td><td align="char" char=".">0.13</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a-i</sup>Least square means in the same column with different superscripts differ (<italic>P</italic> &lt; 0.05).</p></fn><fn><p><sup>1</sup>EHDP = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran dual purpose.</p></fn><fn><p><sup>2</sup>EHCF = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran corn-fed diet.</p></fn><fn><p><sup>3</sup>EHSC = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran sugar cane diet.</p></fn><fn><p><sup>4</sup>HDP = Honduran dual purpose.</p></fn><fn><p><sup>5</sup>HCF = Honduran corn-fed diet.</p></fn><fn><p><sup>6</sup>HSC = Honduran sugar cane diet.</p></fn><fn><p><sup>7</sup>SE = USDA Select.</p></fn><fn><p><sup>8</sup>TC = USDA top (upper 2/3) Choice.</p></fn><fn><p><sup>9</sup>SEM (largest) of the least square means.</p></fn></table-wrap-foot></table-wrap><table-wrap id="tbl7" position="float"><label>Table 7.</label><caption><p>The effects of treatment on least square means for consumer (n = 480) acceptability for each palatability trait</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Treatment</td><td align="center">Tenderness, %</td><td align="center">Juiciness, %</td><td align="center">Flavor liking, %</td><td align="center">Overall liking, %</td></tr></thead><tbody><tr><td> EHDP<sup>1</sup></td><td align="char" char=".">45.05<sup>d</sup></td><td align="char" char=".">51.99<sup>de</sup></td><td align="char" char=".">54.15<sup>d</sup></td><td align="char" char=".">54.53<sup>cd</sup></td></tr><tr><td> EHCF<sup>2</sup></td><td align="char" char=".">97.15<sup>a</sup></td><td align="char" char=".">85.99<sup>a</sup></td><td align="char" char=".">89.16<sup>a</sup></td><td align="char" char=".">86.61<sup>a</sup></td></tr><tr><td> EHSC<sup>3</sup></td><td align="char" char=".">70.09<sup>c</sup></td><td align="char" char=".">67.75<sup>bc</sup></td><td align="char" char=".">67.89<sup>b</sup></td><td align="char" char=".">66.13<sup>b</sup></td></tr><tr><td> HDP<sup>4</sup></td><td align="char" char=".">27.30<sup>e</sup></td><td align="char" char=".">36.55<sup>f</sup></td><td align="char" char=".">35.00<sup>e</sup></td><td align="char" char=".">34.94<sup>e</sup></td></tr><tr><td> HCF<sup>5</sup></td><td align="char" char=".">70.58<sup>c</sup></td><td align="char" char=".">55.86<sup>de</sup></td><td align="char" char=".">57.06<sup>cd</sup></td><td align="char" char=".">61.30<sup>bc</sup></td></tr><tr><td> HSC<sup>6</sup></td><td align="char" char=".">47.01<sup>d</sup></td><td align="char" char=".">44.01<sup>ef</sup></td><td align="char" char=".">42.54<sup>e</sup></td><td align="char" char=".">45.95<sup>de</sup></td></tr><tr><td> SE<sup>7</sup></td><td align="char" char=".">78.96<sup>bc</sup></td><td align="char" char=".">59.64<sup>cd</sup></td><td align="char" char=".">63.01<sup>bcd</sup></td><td align="char" char=".">69.61<sup>b</sup></td></tr><tr><td> TC<sup>8</sup></td><td align="char" char=".">85.53<sup>b</sup></td><td align="char" char=".">72.74<sup>b</sup></td><td align="char" char=".">65.97<sup>bc</sup></td><td align="char" char=".">72.06<sup>b</sup></td></tr><tr><td>SEM<sup>9</sup></td><td align="char" char=".">0.63</td><td align="char" char=".">0.42</td><td align="char" char=".">0.38</td><td align="char" char=".">0.42</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a-f</sup>Least square means in the same column with different superscripts differ (<italic>P</italic> &lt; 0.05).</p></fn><fn><p><sup>1</sup>EHDP = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran dual purpose.</p></fn><fn><p><sup>2</sup>EHCF = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran corn-fed diet.</p></fn><fn><p><sup>3</sup>EHSC = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran sugar cane diet.</p></fn><fn><p><sup>4</sup>HDP = Honduran dual purpose.</p></fn><fn><p><sup>5</sup>HCF = Honduran corn-fed diet.</p></fn><fn><p><sup>6</sup>HSC = Honduran sugar cane diet.</p></fn><fn><p><sup>7</sup>SE = USDA Select.</p></fn><fn><p><sup>8</sup>TC = USDA top (upper 2/3) Choice.</p></fn><fn><p><sup>9</sup>SEM (largest) of the least square means.</p></fn></table-wrap-foot></table-wrap><p>Greater tenderness scores, coupled with greater tenderness acceptability from Honduran consumers could have been due to their acclimation of their domestic beef supply and preference for beef to which they are accustomed. <xref ref-type="bibr" rid="r11">Delgado et al. (2005)</xref> reported a similar trend when comparing U.S. and Mexican beef. In the Delgado study, despite registering a lower shear force value than Northern Mexican beef, USDA Choice was rated similar for tenderness by Mexican consumers, attributing this phenomenon to Mexican beef consumers’ familiarization with the taste, flavor, and aroma of locally produced beef due to the rich tradition of beef consumption in Mexico (<xref ref-type="bibr" rid="r11">Delgado et al., 2005</xref>). Moreover, the higher degree of doneness was more suited to Honduran than U.S. consumers, which also could have influenced tenderness ratings and acceptability between the two countries.</p><p>Previous research has shown that enhancement can improve palatability traits, including tenderness (<xref ref-type="bibr" rid="r21">Jensen et al., 2003</xref>; <xref ref-type="bibr" rid="r46">Scanga et al., 2000</xref>). Improvement of consumer tenderness scores in enhanced samples can be attributed to higher water content, as <xref ref-type="bibr" rid="r37">Offer and Trinick (1983)</xref> highlighted the positive effect of salt and polyphosphates helping myofibrils retain twice the volume and providing the capacity for actin and myosin disassociation, respectively.</p><p>Differences between cattle production systems and diets can affect tenderness, as animals fed a grain-based diet have higher tenderness scores (<xref ref-type="bibr" rid="r35">Nuernberg et al., 2005</xref>). <xref ref-type="bibr" rid="r35">Nuernberg et al. (2005)</xref> proposed that grazing animals have a slower growth rate when compared to animals produced under higher energy diets, thus resulting in tougher meat.</p><p>Differences in all palatability trait scores between EHCF, EHSC, and EHDP may indicate that breed influenced consumer scores. Differences in tenderness between breeds can be a result of variable levels of calpain and calpastatin, as previous research has shown that increasing the percentage of Brahman will increase the calpastatin activity (<xref ref-type="bibr" rid="r40">Pringle et al., 1997</xref>), thus resulting in lower tenderness.</p><p>Despite similar (<italic>P</italic> &gt; 0.05) consumer palatability scores and acceptability percentages between TC and SE in our study, differences in palatability between quality grades in U.S. treatments have been previously reported when comparing strip loin tenderness scores (<xref ref-type="bibr" rid="r9">Corbin et al., 2015</xref>; <xref ref-type="bibr" rid="r36">O’Quinn et al., 2012</xref>; <xref ref-type="bibr" rid="r51">Tedford et al., 2014</xref>).</p></sec><sec><title>Consumer juiciness</title><p>Much like tenderness, both the country where product was evaluated and the treatment independently influenced (<italic>P</italic> &lt; 0.01) juiciness scores, while no interaction was detected (<xref ref-type="table" rid="tbl5">Table 5</xref>; <italic>P</italic> = 0.09); however, those two effects interacted (<italic>P</italic> = 0.04) to impact juiciness acceptability (<xref ref-type="table" rid="tbl6">Table 6</xref>). Honduran consumers rated samples juicier (<italic>P</italic> &lt; 0.01) than U.S. consumers. Regardless of country, EHCF samples had greater (<italic>P</italic> &lt; 0.05) consumer juiciness scores than any other treatment, followed by similar (<italic>P</italic> &gt; 0.05) scores for TC, EHSC, and SE. The treatments that received the lowest juiciness ratings were HDP, EHDP, and HSC. Despite the fact EHDP was enhanced, no differences (<italic>P</italic> &gt; 0.05) in juiciness were detected between HDP and EHDP, indicating that enhancement had negligible effect on EHDP. However, enhancement could be detected by consumers resulting in greater (<italic>P</italic> &lt; 0.05) juiciness for EHCF and EHSC when compared to their non-enhanced counterparts. When examining the percentage of samples rated as acceptable for juiciness, enhancement had a positive effect when comparing all 3 Honduran treatments to their non-enhanced counterparts for both U.S. and Honduran consumers. Moreover, TC was the only treatment that a greater (<italic>P</italic> &lt; 0.05) proportion of U.S. consumers found acceptable for juiciness than Honduran consumers, but those percentages were similar between the two countries for all other treatments (<italic>P</italic> &gt; 0.05) – an interesting finding, given Honduran consumers were less discriminative when scoring juiciness than U.S. consumers.</p><p>Country where product was evaluated influenced juiciness scores, as Honduran consumers gave greater (<italic>P</italic> &lt; 0.05) juiciness scores when compared to U.S consumers. This could be partly attributed to the degree of doneness (77°C) that was selected because Central American consumers prefer well done beef (<xref ref-type="bibr" rid="r30">McDonald, 2009</xref>). Research has revealed that degree of doneness affects consumer attitudes toward beef (<xref ref-type="bibr" rid="r27">Luo et al., 2009</xref>). Previous consumer research in Lubbock, TX has shown most consumers (59.8 to 76.8%) prefer their steaks cooked to medium rare or medium, and as the degree of doneness increases past medium, there is a decline in consumer satisfaction (<xref ref-type="bibr" rid="r8">Claborn et al., 2011</xref>).</p><p>Enhancement can increase palatability scores, specifically when treatments are enhanced with phosphate and salt, compared to the use of water only (<xref ref-type="bibr" rid="r4">Baublits et al., 2005</xref>). Consumer juiciness scores can be positively affected by enhancement (<xref ref-type="bibr" rid="r43">Robbins et al., 2003</xref>; <xref ref-type="bibr" rid="r61">Wicklund et al., 2006</xref>) as well as other production factors such as diet, demonstrating that a diet in which grains have been included will improve palatability of meat (<xref ref-type="bibr" rid="r32">Muir et al., 1998</xref>). Despite the positive effect of enhancement on EHCF and EHSC compared to HCF and HSC, respectively, consumers rated EHCF considerably juicier than EHSC. This difference is likely due to the dietary effect on meat from the HCF treatment.</p></sec><sec><title>Consumer flavor liking</title><p>As seen in <xref ref-type="table" rid="tbl5">Table 5</xref>, no interaction was observed (<italic>P</italic> = 0.15) but both country where product was evaluated and treatment independently influenced (<italic>P</italic> &lt; 0.01) flavor-liking scores; however, treatment and country where product was evaluated interacted (<italic>P</italic> = 0.03) to influence flavor acceptability (<xref ref-type="table" rid="tbl6">Table 6</xref>). Honduran consumers liked the flavor of the beef samples more (<italic>P</italic> &lt; 0.01) than U.S. consumers (<xref ref-type="table" rid="tbl5">Table 5</xref>). As with tenderness and juiciness, EHCF was rated with the highest (<italic>P</italic> &lt; 0.05) flavor liking score compared to all other treatments. Once again, TC and SE were rated similarly (<italic>P</italic> &gt; 0.05) for flavor liking along with EHSC and HCF, while flavor liking for HDP was lower (<italic>P</italic> &lt; 0.05) than all other treatments, except HSC. A trend similar to that observed for juiciness acceptability was noted for flavor acceptability as well. Again, TC was the only treatment with a greater (<italic>P</italic> &lt; 0.05) proportion of U.S. consumers finding it acceptable for flavor than Honduran consumers (<xref ref-type="table" rid="tbl6">Table 6</xref>), with the remaining percentages being similar between the 2 countries for all other treatments (<italic>P</italic> &gt; 0.05). Also, when examining the percentage of samples rated as acceptable for flavor, enhancement had a positive effect when comparing all three Honduran treatments to their non-enhanced counterparts for both U.S. and Honduran consumers.</p><p>Greater flavor liking scores from Honduran consumers could be a result of differences in production systems between Honduras and U.S, as more than 50% of Hispanic consumers prefer pasture fed beef (<xref ref-type="bibr" rid="r27">Luo et al., 2009</xref>). On the other hand, U.S consumers typically consuming conventional beef have shown to perceive grass fed beef flavors as “intense” (<xref ref-type="bibr" rid="r29">Martz, 2000</xref>). Despite previous results showing differences in flavor liking between TC and SE top loins steaks (<xref ref-type="bibr" rid="r9">Corbin et al., 2015</xref>; <xref ref-type="bibr" rid="r19">Hunt et al., 2014</xref>; <xref ref-type="bibr" rid="r51">Tedford et al., 2014</xref>), TC and Select were rated similarly (<italic>P</italic> &gt; 0.05) for flavor liking in our current study. However, differences in beef production systems, such as those in the U.S. compared to those in Central America, can affect flavor (<xref ref-type="bibr" rid="r49">Smith et al., 1985</xref>; <xref ref-type="bibr" rid="r45">Savell et al., 1987</xref>). Beef from animals raised on a finishing diet with grains tend to deposit more fat (<xref ref-type="bibr" rid="r13">Duckett et al., 2007</xref>; <xref ref-type="bibr" rid="r12">Duckett et al., 2013</xref>), positively affecting flavor (<xref ref-type="bibr" rid="r52">Umberger et al., 2000</xref>). The grain finishing diet of HCF may have been responsible for similar scores between HCF and U.S. treatments. Although biological type can certainly influence carcass fat deposition (<xref ref-type="bibr" rid="r20">Ito et al., 2012</xref>), the grain-based diet of HCF had a positive effect on flavor liking despite the <italic>Bos indicus</italic> influence of those cattle. Grass fed beef has been reported by U.S. consumers to have less acceptance for beef flavor due to off-flavors (<xref ref-type="bibr" rid="r12">Duckett et al., 2013</xref>). Despite previous evidence of a reduction of “grassy” off-flavor from including grain in the animal diet (<xref ref-type="bibr" rid="r25">Larick et al., 1987</xref>), in our study no differences in flavor liking scores were observed between HSC and HDP, whereas HCF was more liked than either.</p><p>Enhancement had a positive effect on flavor liking as each enhanced treatment was scored greater than its respective non-enhanced counterpart. Improvement of flavor may be attributed to salt in the brine solution. Previous researchers (<xref ref-type="bibr" rid="r38">Papadopoulos and Miller, 1991</xref>; <xref ref-type="bibr" rid="r57">Vote et al., 2000</xref>) have shown increased flavor ratings from enhancement with brine solutions containing salt (0.5%).</p></sec><sec><title>Consumer overall liking</title><p>Both country where the product was evaluated and the treatment influenced (<italic>P</italic> &lt; 0.01) overall liking (<xref ref-type="table" rid="tbl5">Table 5</xref>) but only treatment influenced (<italic>P</italic> &lt; 0.01) overall acceptability (<xref ref-type="table" rid="tbl7">Table 7</xref>), as overall acceptability was similar between the 2 countries (<italic>P</italic> = 0.20). No interactions for overall liking (<italic>P</italic> = 0.42) or overall acceptability (<italic>P</italic> = 0.13) were observed. Honduran consumers had greater (<italic>P</italic> &lt; 0.01) scores for overall liking than U.S. consumers. For each Honduran treatment, enhancement improved (<italic>P</italic> &lt; 0.05) both overall liking and overall acceptability compared to the non-enhanced version of the respective treatment. Once again, EHCF was more liked than any other treatment, followed by similar (<italic>P</italic> &gt; 0.05) scores for both U.S. treatments and EHSC. Despite differences in quality grades, consumers rated TC and SE similarly (<italic>P</italic> &gt; 0.05) in overall liking as well as the percentage of consumers finding TC and SE acceptable overall. The HDP samples had the lowest (<italic>P</italic> &gt; 0.05) consumer overall liking scores and the lowest (<italic>P</italic> &gt; 0.05) overall acceptability, with the exception that a similar proportion of consumers found HSC acceptable overall.</p><p>Consumer ratings in tenderness, juiciness and flavor are related to consumer overall liking ratings (<xref ref-type="bibr" rid="r39">Platter et al., 2003</xref>); however, other researchers suggest overall liking scores are attributed more so to flavor liking than tenderness or juiciness (<xref ref-type="bibr" rid="r9">Corbin et al., 2015</xref>; <xref ref-type="bibr" rid="r34">Neely et al., 1998</xref>) when tenderness reaches an acceptable level. Low values in tenderness, juiciness and flavor likely influenced HDP achieving the lowest overall liking scores and overall acceptability.</p></sec><sec><title>Willingness to pay</title><p>As seen in <xref ref-type="table" rid="tbl8">Table 8</xref>, no interactions between country where product was evaluated and treatment were observed for the percentage of consumers willingness to pay at each of the 4 values (<italic>P</italic> ≥ 0.09). Treatment influenced (<italic>P</italic> &lt; 0.01) the percentage of consumers willing to pay at each value ($0, 3, 6, and 10/0.45 kg), but country where product was evaluated only influenced the proportion of consumers willing to pay for 3 of the 4 values ($0, 3, and 10/0.45 kg). Consumers were least willing to pay for HDF and HSC, as the greatest percentage of consumers indicated they would not pay for those treatments ($0/0.45 kg). Consumers were willing to pay the most for EHCF, as the highest percentage of consumers indicated they would pay $6 or 10/0.45 kg for EHCF compared to all other treatments, followed by a similar percentage of consumers willing to pay $10/0.45 kg for TC, SE, and EHSC or $6/0.45 kg for EHSC, SE, TC, and HCF. A similar (<italic>P</italic> &gt; 0.05) percentage of consumers indicated they would pay $3/0.45 kg for HCF, TC, SE, and EHSC, which is in alignment for intermediate palatability scores and acceptability for those 4 treatments. Consequently, consumers were most often willing to pay $3/0.45 kg as opposed to $6/0.45 kg for HCF, TC, SE, and EHSC. A positive effect of enhancement of Honduran treatments was observed, as the percentage of consumers willing to pay $0/0.45 kg was reduced with a concurrent increase in the proportion of consumers willing to pay $3/0.45 kg and $6/0.45 kg compared to the non-enhanced version of those treatments.</p><table-wrap id="tbl8" position="float"><label>Table 8.</label><caption><p>Percentage of consumers (n = 480) willingness to pay ($/0.45 kg) for U.S. and Honduran sourced treatments</p></caption><table frame="hsides" rules="groups"><thead><tr><td>Treatment</td><td align="center">$0</td><td align="center">$3</td><td align="center">$6</td><td align="center">$10</td></tr></thead><tbody><tr><td> EHDP<sup>1</sup></td><td align="char" char=".">41.91<sup>b</sup></td><td align="char" char=".">38.52<sup>cd</sup></td><td align="char" char=".">14.29<sup>cd</sup></td><td align="char" char=".">1.02<sup>c</sup></td></tr><tr><td> EHCF<sup>2</sup></td><td align="char" char=".">3.91<sup>e</sup></td><td align="char" char=".">32.62<sup>d</sup></td><td align="char" char=".">44.97<sup>a</sup></td><td align="char" char=".">15.02<sup>a</sup></td></tr><tr><td> EHSC<sup>3</sup></td><td align="char" char=".">24.55<sup>cd</sup></td><td align="char" char=".">42.02<sup>abc</sup></td><td align="char" char=".">27.94<sup>b</sup></td><td align="char" char=".">2.24<sup>bc</sup></td></tr><tr><td> HDP<sup>4</sup></td><td align="char" char=".">56.60<sup>a</sup></td><td align="char" char=".">34.98<sup>cd</sup></td><td align="char" char=".">6.19<sup>e</sup></td><td align="char" char=".">1.09<sup>c</sup></td></tr><tr><td> HCF<sup>5</sup></td><td align="char" char=".">26.59<sup>c</sup></td><td align="char" char=".">50.29<sup>a</sup></td><td align="char" char=".">18.91<sup>bc</sup></td><td align="char" char=".">1.22<sup>c</sup></td></tr><tr><td> HSC<sup>6</sup></td><td align="char" char=".">45.08<sup>a</sup></td><td align="char" char=".">39.56<sup>bcd</sup></td><td align="char" char=".">10.94<sup>de</sup></td><td align="char" char=".">1.56<sup>c</sup></td></tr><tr><td> SE<sup>7</sup></td><td align="char" char=".">22.62<sup>cd</sup></td><td align="char" char=".">47.62<sup>a</sup></td><td align="char" char=".">23.90<sup>b</sup></td><td align="char" char=".">2.50<sup>bc</sup></td></tr><tr><td> TC<sup>8</sup></td><td align="char" char=".">15.62<sup>d</sup></td><td align="char" char=".">49.18<sup>a</sup></td><td align="char" char=".">23.63<sup>b</sup></td><td align="char" char=".">5.13<sup>b</sup></td></tr><tr><td>SEM<sup>9</sup></td><td align="char" char=".">0.37</td><td align="char" char=".">0.17</td><td align="char" char=".">0.35</td><td align="char" char=".">0.76</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td></tr><tr><td>Country</td></tr><tr><td> U.S.</td><td align="char" char=".">28.15<sup>a</sup></td><td align="char" char=".">36.90<sup>b</sup></td><td align="char" char=".">19.91<sup>a</sup></td><td align="char" char=".">3.79<sup>a</sup></td></tr><tr><td> Honduras</td><td align="char" char=".">23.27<sup>b</sup></td><td align="char" char=".">46.50<sup>a</sup></td><td align="char" char=".">18.34<sup>a</sup></td><td align="char" char=".">1.50<sup>b</sup></td></tr><tr><td>SEM<sup>9</sup></td><td align="char" char=".">0.09</td><td align="char" char=".">0.06</td><td align="char" char=".">0.09</td><td align="char" char=".">0.24</td></tr><tr><td><italic>P</italic>-value</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">&lt; 0.01</td><td align="char" char=".">0.27</td><td align="char" char=".">&lt; 0.01</td></tr><tr><td><italic>P</italic>-value<sup>10</sup> (T × C)</td><td align="char" char=".">0.60</td><td align="char" char=".">0.90</td><td align="char" char=".">0.87</td><td align="char" char=".">0.09</td></tr></tbody></table><table-wrap-foot><fn><p><sup>a-e</sup>Least square means in the same column with different superscripts differ, <italic>P</italic> &lt; 0.05.</p></fn><fn><p><sup>1</sup>EHDP = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran dual purpose.</p></fn><fn><p><sup>2</sup>EHCF = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran corn-fed diet.</p></fn><fn><p><sup>3</sup>EHSC = Enhanced (112% with a target concentration of 0.25% tripolyphosphate and 0.50% salt of the final product) Honduran sugar cane diet.</p></fn><fn><p><sup>4</sup>HDP = Honduran dual purpose.</p></fn><fn><p><sup>5</sup>HCF = Honduran corn-fed diet.</p></fn><fn><p><sup>6</sup>HSC = Honduran sugar cane diet.</p></fn><fn><p><sup>7</sup>SE = USDA Select.</p></fn><fn><p><sup>8</sup>TC = USDA top (upper 2/3) Choice.</p></fn><fn><p><sup>9</sup>SEM (largest) of the least square means.</p></fn><fn><p><sup>10</sup><italic>P</italic>-value of treatment × country interaction.</p></fn></table-wrap-foot></table-wrap><p>A greater (<italic>P</italic> &gt; 0.01) percentage of U.S consumers were willing to pay $0/0.45 kg (<italic>P</italic> &lt; 0.01) and $10/0.45 kg when compared to Honduran consumers. Consequently, a greater (<italic>P</italic> &lt; 0.01) percentage of Honduran consumers were willing to pay $3/0.45 kg compared to U.S. consumers. However, there was no difference in the proportion of Honduran and U.S. consumers that were willing to pay $6/0.45 kg (<italic>P</italic> &gt; 0.01).</p><p>Consumers’ willingness to pay has been linked to overall liking scores in previous studies (<xref ref-type="bibr" rid="r24">Kukowski et al., 2005</xref>; <xref ref-type="bibr" rid="r41">Reicks et al., 2011</xref>). Consumers have reportedly been willing to pay more as tenderness increases (<xref ref-type="bibr" rid="r5">Boleman et al.,1997</xref>). In this study, enhanced treatments were scored greater for overall liking, partially due to tenderness, which can explain differences in willingness to pay between enhanced and non-enhanced Honduran treatments.</p><p>Results suggest that enhancement increases the value consumers are willing to pay for Honduran treatments. According to <xref ref-type="bibr" rid="r58">WageIndicator (2015)</xref>, Honduran minimum wage was lower, ranging from 22.44 to 37.01 L. ($1.04 USD to $1.71 USD) depending on the industry and the number of employees at the firm than in the U.S ($7.25/hour; <xref ref-type="bibr" rid="r53">USDL, 2017</xref>). However, a higher percentage of Honduran consumers were willing to pay $3/0.45 kg and a higher percentage of U.S consumers were willing to pay $0/0.45 kg. These results could suggest that U.S consumer beef quality expectations might be higher than Honduran consumers because if consumers indicated they were willing to pay $0/0.45 kg, those consumers found the sample unsatisfactory in some regard as they would not pay any money for that sample.</p></sec><sec><title>Conclusions</title><p>Greatest palatability traits scores were obtained in EHCF treatment in U.S. and Honduras. Enhancement of Honduran treatments had a positive effect on palatability traits, as well as acceptability of each of those traits. Honduran consumers ranked all palatability traits more favorably than U.S. consumers along with greater acceptability for tenderness. Regardless of the differences in breeds, including diets utilizing grain and/or by-products along with enhancement resulted in greater palatability scores. The U.S. consumers were willing to pay more indicating higher expectations for beef quality.</p></sec></sec></body><back><ref-list><title>Literature Cited</title><ref id="r1"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Allingham</surname><given-names>P. G.</given-names></name><name><surname>Harper</surname><given-names>G. S.</given-names></name><name><surname>Hunter</surname><given-names>R. A.</given-names></name></person-group>. <year>1998</year>. <article-title>Effect of growth path on the tenderness of the semitendinosus muscle of Brahman-cross steers</article-title>. <source>Meat Sci.</source> <volume>48</volume>:<fpage>65</fpage>–<lpage>73</lpage>. <comment>doi:10.1016/S0309-1740(97)00076-4</comment><uri>http://dx.doi.org/10.1016/S0309-1740(97)00076-4</uri></citation></ref><ref id="r2"><citation citation-type="book"><person-group person-group-type="author"><collab>American Meat Science Association</collab></person-group>. <year>2015</year>. <article-title>Research guidelines for cookery, sensory evaluation, and instrumental tenderness measurements of meat</article-title>. <publisher-name>Version 1.0. Am. Meat Sci. Assoc</publisher-name>. <publisher-loc>Champaign, IL</publisher-loc>.</citation></ref><ref id="r3"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Anderson</surname><given-names>S</given-names></name></person-group>. <year>2007</year>. <article-title>Determination of fat, moisture, and protein in meat and meat products using the FOSS FoodScan near-infrared spectrophotometer with FOSS Artificial Neural Network Calibration Model and Associated Database: Collaborative study</article-title>. <source>J. AOAC Int</source>. <volume>90</volume>:<fpage>1073</fpage>–<lpage>1083</lpage>.<ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000248520200028&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r4"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Baublits</surname><given-names>R. T.</given-names></name><name><surname>Pohlman</surname><given-names>F. W.</given-names></name><name><surname>Brown</surname><given-names>A. H.</given-names></name><name><surname>Johnson</surname><given-names>Z. B.</given-names></name></person-group>. <year>2005</year>. <article-title>Effects of sodium chloride, phosphate type and concentration, and pump rate on beef biceps femoris quality and sensory characteristics</article-title>. <source>Meat Sci.</source> <volume>70</volume>:<fpage>205</fpage>–<lpage>214</lpage>. <comment>doi:10.1016/j.meatsci.2004.12.011</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2004.12.011</uri></citation></ref><ref id="r5"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Boleman</surname><given-names>S. J.</given-names></name><name><surname>Boleman</surname><given-names>S. L.</given-names></name><name><surname>Miller</surname><given-names>R. K.</given-names></name><name><surname>Taylor</surname><given-names>J. F.</given-names></name><name><surname>Cross</surname><given-names>H. R.</given-names></name><name><surname>Wheeler</surname><given-names>T. L.</given-names></name><name><surname>Koohmaraie</surname><given-names>M.</given-names></name><name><surname>Shackelford</surname><given-names>S. D.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name><name><surname>West</surname><given-names>R. L.</given-names></name><name><surname>Johnson</surname><given-names>D. D.</given-names></name><name><surname>Savell</surname><given-names>J. W.</given-names></name></person-group>. <year>1997</year>. <article-title>Consumer evaluation of beef of known categories of tenderness</article-title>. <source>J. Anim. Sci.</source> <volume>75</volume>:<fpage>1521</fpage>–<lpage>1524</lpage>. <comment>doi:10.2527/1997.7561521x</comment><uri>http://dx.doi.org/10.2527/1997.7561521x</uri></citation></ref><ref id="r6"><citation citation-type="book"><person-group person-group-type="author"><name><surname>Bueso</surname><given-names>M. E</given-names></name></person-group>. <year>2015</year>. <article-title>Honduran and U.S. consumer assessment of beef strip loin steaks from grass and grain finished cattle</article-title>. <publisher-name>M. S. Thesis, Texas Tech University</publisher-name>, <publisher-loc>Lubbock</publisher-loc>.</citation></ref><ref id="r7"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Campo</surname><given-names>M. M.</given-names></name><name><surname>Santolaria</surname><given-names>P.</given-names></name><name><surname>Sañudo</surname><given-names>C.</given-names></name><name><surname>Lepetit</surname><given-names>J.</given-names></name><name><surname>Olleta</surname><given-names>J. L.</given-names></name><name><surname>Panea</surname><given-names>B.</given-names></name><name><surname>Albertí</surname><given-names>P.</given-names></name></person-group>. <year>2000</year>. <article-title>Assessment of breed type and ageing time effects on beef meat quality using two different texture devices</article-title>. <source>Meat Sci.</source> <volume>55</volume>:<fpage>371</fpage>–<lpage>378</lpage>. <comment>doi:10.1016/S0309-1740(99)00162-X</comment><uri>http://dx.doi.org/10.1016/S0309-1740(99)00162-X</uri></citation></ref><ref id="r8"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Claborn</surname><given-names>S. W.</given-names></name><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>Brooks</surname><given-names>J. C.</given-names></name><name><surname>Rathmann</surname><given-names>R. J.</given-names></name><name><surname>Ramsey</surname><given-names>C. B.</given-names></name><name><surname>Thompson</surname><given-names>L. D.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name></person-group>. <year>2011</year>. <article-title>Consumer evaluation of the palatability of USDA Select, USDA Choice, and Certified Angus Beef strip loin steaks from retail markets in Lubbock, Texas, USA</article-title>. <source>J. Food Qual</source>. <volume>34</volume>:<fpage>425</fpage>–<lpage>434</lpage>.<uri>http://dx.doi.org/10.1111/j.1745-4557.2011.00415.x</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000297949000007&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r9"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Corbin</surname><given-names>C. H.</given-names></name><name><surname>O’Quinn</surname><given-names>T. G.</given-names></name><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>Legako</surname><given-names>J. F.</given-names></name><name><surname>Hunt</surname><given-names>M. R.</given-names></name><name><surname>Dinh</surname><given-names>T. T. N.</given-names></name><name><surname>Rathmann</surname><given-names>R.</given-names></name><name><surname>Brooks</surname><given-names>J., J. C.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name></person-group>. <year>2015</year>. <article-title>Sensory evaluation of tender beef strip loin steaks of varying marbling levels and quality treatments</article-title>. <source>Meat Sci.</source> <volume>100</volume>:<fpage>24</fpage>–<lpage>31</lpage>. <comment>doi:10.1016/j.meatsci.2014.09.009</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2014.09.009</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000347765700005&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r10"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Crouse</surname><given-names>J. D.</given-names></name><name><surname>Cundiff</surname><given-names>L. V.</given-names></name><name><surname>Koch</surname><given-names>R. M.</given-names></name><name><surname>Koohmaraie</surname><given-names>M.</given-names></name><name><surname>Seideman</surname><given-names>S. C.</given-names></name></person-group>. <year>1989</year>. <article-title>Comparisons of and Inheritance for Carcass Beef Characteristics and Meat Palatability</article-title>. <source>J. Anim. Sci.</source> <volume>67</volume>:<fpage>2661</fpage>–<lpage>2668</lpage>. <comment>doi:10.2527/jas1989.67102661x</comment><uri>http://dx.doi.org/10.2527/jas1989.67102661x</uri></citation></ref><ref id="r11"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Delgado</surname><given-names>E. J.</given-names></name><name><surname>Rubio</surname><given-names>M. S.</given-names></name><name><surname>Iturbe</surname><given-names>F. A.</given-names></name><name><surname>Méndez</surname><given-names>R. D.</given-names></name><name><surname>Cassís</surname><given-names>L.</given-names></name><name><surname>Rosiles</surname><given-names>R</given-names></name></person-group>. <year>2005</year>. <article-title>Composition and quality of Mexican and imported retail beef in Mexico</article-title>. <source>Meat Sci.</source> <volume>69</volume>:<fpage>465</fpage>–<lpage>471</lpage>. <comment>doi:10.1016/j.meatsci.2004.10.003</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2004.10.003</uri></citation></ref><ref id="r12"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Duckett</surname><given-names>S. K.</given-names></name><name><surname>Neel</surname><given-names>J. P. S.</given-names></name><name><surname>Lewis</surname><given-names>R. M.</given-names></name><name><surname>Fontenot</surname><given-names>J. P.</given-names></name><name><surname>Clapham</surname><given-names>W. M.</given-names></name></person-group>. <year>2013</year>. <article-title>Effects of forage species or concentrate finishing on animal performance, carcass and meat quality</article-title>. <source>J. Anim. Sci.</source> <volume>91</volume>:<fpage>1454</fpage>–<lpage>1467</lpage>. <comment>doi:10.2527/jas.2012-5914</comment><uri>http://dx.doi.org/10.2527/jas.2012-5914</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000319691500045&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r13"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Duckett</surname><given-names>S. K.</given-names></name><name><surname>Neel</surname><given-names>J. P. S.</given-names></name><name><surname>Sonon</surname><given-names>R. N.</given-names></name><name><surname>Fontenot</surname><given-names>J. P.</given-names></name><name><surname>Clapham</surname><given-names>W. M.</given-names></name><name><surname>Scaglia</surname><given-names>G.</given-names></name></person-group>. <year>2007</year>. <article-title>Effects of winter stocker growth rate and finishing system on: II. Ninth-tenth-eleventh-rib composition, muscle color, and palatability</article-title>. <source>J. Anim. Sci.</source> <volume>85</volume>:<fpage>2691</fpage>–<lpage>2698</lpage>. <comment>doi:10.2527/jas.2006-734</comment><uri>http://dx.doi.org/10.2527/jas.2006-734</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000249557700035&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r14"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Emerson</surname><given-names>M. R.</given-names></name><name><surname>Woerner</surname><given-names>D. R.</given-names></name><name><surname>Belk</surname><given-names>K. E.</given-names></name><name><surname>Tatum</surname><given-names>J. D.</given-names></name></person-group>. <year>2013</year>. <article-title>Effectiveness of USDA instrument-based marbling measurements for categorizing beef carcasses according to differences in longissimus muscle sensory attributes</article-title>. <source>J. Anim. Sci.</source> <volume>91</volume>:<fpage>1024</fpage>–<lpage>1034</lpage>. <comment>doi:10.2527/jas.2012-5514</comment><uri>http://dx.doi.org/10.2527/jas.2012-5514</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000319687800055&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r15"><citation citation-type="web"><person-group person-group-type="author"><collab>FAO</collab></person-group>. <year>2015</year>. <article-title>Food Security Indicators</article-title>. <comment><ext-link ext-link-type="uri" xlink:href="http://www.fao.org/economic/ess/ess-fs/ess- fadata/en/#.VwLquOuuVVI" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.fao.org/economic/ess/ess-fs/ess- fadata/en/#.VwLquOuuVVI</ext-link> (accessed 15 October 2015)</comment>.</citation></ref><ref id="r16"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>Hilton</surname><given-names>G. G.</given-names></name><name><surname>Mateescu</surname><given-names>R. G.</given-names></name><name><surname>Morgan</surname><given-names>J. B.</given-names></name><name><surname>Reecy</surname><given-names>J. M.</given-names></name><name><surname>Tait</surname><given-names>R. G.</given-names><suffix>Jr</suffix></name><name><surname>Beitz</surname><given-names>D. C.</given-names></name><name><surname>Duan</surname><given-names>Q.</given-names></name><name><surname>Schoonmaker</surname><given-names>J. P.</given-names></name><name><surname>Mayes</surname><given-names>M. S.</given-names></name><name><surname>Drewnoski</surname><given-names>M. E.</given-names></name><name><surname>Liu</surname><given-names>Q.</given-names></name><name><surname>VanOverbeke</surname><given-names>D. L.</given-names></name></person-group>. <year>2011</year>. <article-title>Estimation of relationships between mineral concentration and fatty acid composition of longissimus muscle and beef palatability traits</article-title>. <source>J. Anim. Sci.</source> <volume>89</volume>:<fpage>2849</fpage>–<lpage>2858</lpage>. <comment>doi:10.2527/jas.2010-3497</comment><uri>http://dx.doi.org/10.2527/jas.2010-3497</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000293893000021&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r17"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Highfill</surname><given-names>C. M.</given-names></name><name><surname>Esquivel-Font</surname><given-names>O.</given-names></name><name><surname>Dikeman</surname><given-names>M. E.</given-names></name><name><surname>Kropf</surname><given-names>D. H.</given-names></name></person-group>. <year>2012</year>. <article-title>Tenderness profiles of ten muscles from F1 <italic>Bos indicus</italic> x <italic>Bos taurus</italic> and <italic>Bos taurus</italic> cattle cooked as steaks and roasts</article-title>. <source>Meat Sci.</source> <volume>90</volume>:<fpage>881</fpage>–<lpage>886</lpage>. <comment>doi:10.1016/j.meatsci.2011.11.022</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2011.11.022</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000300030200002&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r18"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Hiner</surname><given-names>R. L.</given-names></name><name><surname>Hankins</surname><given-names>O. G.</given-names></name></person-group>. <year>1950</year>. <article-title>The Tenderness of Beef in Relation to Different Muscles and Age in the Animal</article-title>. <source>J. Anim. Sci.</source> <volume>9</volume>:<fpage>347</fpage>–<lpage>353</lpage>. <comment>doi:10.2527/jas1950.93347x</comment><uri>http://dx.doi.org/10.2527/jas1950.93347x</uri></citation></ref><ref id="r19"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Hunt</surname><given-names>M. R.</given-names></name><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>O’Quinn</surname><given-names>T. G.</given-names></name><name><surname>Corbin</surname><given-names>C. H.</given-names></name><name><surname>Legako</surname><given-names>J. F.</given-names></name><name><surname>Rathmann</surname><given-names>R. J.</given-names></name><name><surname>Brooks</surname><given-names>J. C.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name></person-group>. <year>2014</year>. <article-title>Consumer assessment of beef palatability from four beef muscles from USDA Choice and Select graded carcasses</article-title>. <source>Meat Sci.</source> <volume>98</volume>:<fpage>1</fpage>–<lpage>8</lpage>. <comment>doi:10.1016/j.meatsci.2014.04.004</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2014.04.004</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000337782800001&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r20"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Ito</surname><given-names>R. H.</given-names></name><name><surname>do Prado</surname><given-names>I. N.</given-names></name><name><surname>Rotta</surname><given-names>P. P.</given-names></name><name><surname>de Oliveira</surname><given-names>M. G.</given-names></name><name><surname>do Prado</surname><given-names>R. M.</given-names></name><name><surname>Moletta</surname><given-names>J. L.</given-names></name></person-group>. <year>2012</year>. <article-title>Carcass characteristics, chemical composition and fatty acid profile of longissimus muscle of young bulls from four genetic groups finished in feedlot</article-title>. <source>Rev. Bras. Zootec</source>. <volume>41</volume>:<fpage>384</fpage>–<lpage>391</lpage>. <comment>doi:10.1590/S1516-35982012000200022</comment><uri>http://dx.doi.org/10.1590/S1516-35982012000200022</uri></citation></ref><ref id="r21"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Jensen</surname><given-names>J. M.</given-names></name><name><surname>Robbins</surname><given-names>K. L.</given-names></name><name><surname>Ryan</surname><given-names>K. J.</given-names></name><name><surname>Homco-Ryan</surname><given-names>C.</given-names></name><name><surname>McKeith</surname><given-names>F. K.</given-names></name><name><surname>Brewer</surname><given-names>M. S.</given-names></name></person-group>. <year>2003</year>. <article-title>Effects of lactic and acetic acid salts on quality characteristics of enhanced pork during retail display</article-title>. <source>Meat Sci.</source> <volume>63</volume>:<fpage>501</fpage>–<lpage>508</lpage>. <comment>doi:10.1016/S0309-1740(02)00111-0</comment><uri>http://dx.doi.org/10.1016/S0309-1740(02)00111-0</uri></citation></ref><ref id="r22"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Koch</surname><given-names>R. M.</given-names></name><name><surname>Dikeman</surname><given-names>M. E.</given-names></name><name><surname>Allen</surname><given-names>D. M.</given-names></name><name><surname>May</surname><given-names>M.</given-names></name><name><surname>Crouse</surname><given-names>J. D.</given-names></name><name><surname>Campion</surname><given-names>D. R.</given-names></name></person-group>. <year>1976</year>. <article-title>Characterization of Biological Types of Cattle III. Carcass Composition, Quality and Palatability</article-title>. <source>J. Anim. Sci.</source> <volume>43</volume>:<fpage>48</fpage>–<lpage>62</lpage>. <comment>doi:10.2527/jas1976.43148x</comment><uri>http://dx.doi.org/10.2527/jas1976.43148x</uri></citation></ref><ref id="r23"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Koch</surname><given-names>R. M.</given-names></name><name><surname>Dikeman</surname><given-names>M. E.</given-names></name><name><surname>Lipsey</surname><given-names>R. J.</given-names></name><name><surname>Allen</surname><given-names>D. M.</given-names></name><name><surname>Crouse</surname><given-names>J. D.</given-names></name></person-group>. <year>1979</year>. <article-title>Characterization of Biological Types of Cattle- Cycle II: III. Carcass Composition, Quality and Palatability</article-title>. <source>J. Anim. Sci.</source> <volume>49</volume>:<fpage>448</fpage>–<lpage>460</lpage>. <comment>doi:10.2527/jas1979.492448x</comment><uri>http://dx.doi.org/10.2527/jas1979.492448x</uri></citation></ref><ref id="r24"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Kukowski</surname><given-names>A. C.</given-names></name><name><surname>Maddock</surname><given-names>R. J.</given-names></name><name><surname>Wulf</surname><given-names>D. M.</given-names></name><name><surname>Fausti</surname><given-names>S. W.</given-names></name><name><surname>Taylor</surname><given-names>G. L.</given-names></name></person-group>. <year>2005</year>. <article-title>Evaluating consumer acceptability and willingness to pay for various beef chuck muscles</article-title>. <source>J. Anim. Sci.</source> <volume>83</volume>:<fpage>2605</fpage>–<lpage>2610</lpage>. <comment>doi:10.2527/2005.83112605x</comment><uri>http://dx.doi.org/10.2527/2005.83112605x</uri></citation></ref><ref id="r25"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Larick</surname><given-names>D. K.</given-names></name><name><surname>Hedrick</surname><given-names>H. B.</given-names></name><name><surname>Bailey</surname><given-names>M. E.</given-names></name><name><surname>Williams</surname><given-names>J. E.</given-names></name><name><surname>Hancock</surname><given-names>D. L.</given-names></name><name><surname>Garner</surname><given-names>G. B.</given-names></name><name><surname>Morrow</surname><given-names>R. E.</given-names></name></person-group>. <year>1987</year>. <article-title>Flavor Constituents of Beef as Influenced by Forage- and Grain-Feeding</article-title>. <source>J. Food Sci</source>. <volume>52</volume>:<fpage>245</fpage>–<lpage>251</lpage>. <comment>doi:10.1111/j.1365-2621.1987.tb06585.x</comment><uri>http://dx.doi.org/10.1111/j.1365-2621.1987.tb06585.x</uri></citation></ref><ref id="r26"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Lee</surname><given-names>N.</given-names></name><name><surname>Sharma</surname><given-names>V.</given-names></name><name><surname>Mohan</surname><given-names>A.</given-names></name><name><surname>Singh</surname><given-names>R.</given-names></name></person-group>. <year>2014</year>. <article-title>Injection enhancement of beef strip loins with solutions containing sodium tripolyphosphate, carrageenan, sea salt and potassium lactate in combination to improve sensory traits and color stability of beef strip loins</article-title>. <source>Meat Sci.</source> <volume>96</volume>:<fpage>477</fpage>. <comment>doi:10.1016/j.meatsci.2013.07.118</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2013.07.119</uri></citation></ref><ref id="r27"><citation citation-type="book"><person-group person-group-type="author"><name><surname>Luo</surname><given-names>J.</given-names></name><name><surname>Mainville</surname><given-names>D.</given-names></name><name><surname>You</surname><given-names>W.</given-names></name><name><surname>Nagya</surname><given-names>R. M.</given-names></name></person-group>. <year>2009</year>. <article-title>Hispanic consumers’ preferences and willingness to pay for pasture fed beef in Virginia</article-title>. <publisher-name>Virginia Tech</publisher-name>, <publisher-loc>Blacksburg, VA</publisher-loc>.</citation></ref><ref id="r28"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Maughan</surname><given-names>C.</given-names></name><name><surname>Tansawat</surname><given-names>R.</given-names></name><name><surname>Cornforth</surname><given-names>D.</given-names></name><name><surname>Ward</surname><given-names>R.</given-names></name><name><surname>Martini</surname><given-names>S.</given-names></name></person-group>. <year>2012</year>. <article-title>Development of a beef flavor lexicon and its application to compare the flavor profile and consumer acceptance of rib steaks from grass- or grain-fed cattle</article-title>. <source>Meat Sci.</source> <volume>90</volume>:<fpage>116</fpage>–<lpage>121</lpage>. <comment>doi:10.1016/j.meatsci.2011.06.006</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2011.06.006</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000296003400017&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r29"><citation citation-type="book"><person-group person-group-type="author"><name><surname>Martz</surname><given-names>F</given-names></name></person-group>. <year>2000</year>. <article-title>Pasture based finishing of cattle and eating quality of beef</article-title>. <publisher-name>Forage Systems Research Center, University of Missouri</publisher-name>. <comment><ext-link ext-link-type="uri" xlink:href="https://fsrc.missouri.edu/pasture-based-finishing-of-cattle-and-eating-quality-of-beef/" xmlns:xlink="http://www.w3.org/1999/xlink">https://fsrc.missouri.edu/pasture-based-finishing-of-cattle-and-eating-quality-of-beef/</ext-link> (accessed 15 October 2015)</comment>.</citation></ref><ref id="r30"><citation citation-type="book"><person-group person-group-type="author"><name><surname>McDonald</surname><given-names>M. R</given-names></name></person-group>. <year>2009</year>. <article-title>Food culture in Central America</article-title>. <publisher-name>Greenwood Press</publisher-name>, <publisher-loc>Santa Barbara, CA</publisher-loc>.</citation></ref><ref id="r31"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Miller</surname><given-names>M. F.</given-names></name><name><surname>Hoover</surname><given-names>L. C.</given-names></name><name><surname>Cook</surname><given-names>K. D.</given-names></name><name><surname>Guerra</surname><given-names>A. L.</given-names></name><name><surname>Huffman</surname><given-names>K. L.</given-names></name><name><surname>Tinney</surname><given-names>K.</given-names></name><name><surname>Ramsey</surname><given-names>C. B.</given-names></name><name><surname>Brittin</surname><given-names>H. C.</given-names></name><name><surname>Huffman</surname><given-names>L. M.</given-names></name></person-group>. <year>1995</year>. <article-title>Consumer Acceptability of Beef Steak Tenderness in the Home and Restaurant</article-title>. <source>J. Food Sci</source>. <volume>60</volume>:<fpage>963</fpage>–<lpage>965</lpage>. <comment>doi:10.1111/j.1365-2621.1995.tb06271.x</comment><uri>http://dx.doi.org/10.1111/j.1365-2621.1995.tb06271.x</uri></citation></ref><ref id="r32"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Muir</surname><given-names>P. D.</given-names></name><name><surname>Deaker</surname><given-names>J. M.</given-names></name><name><surname>Bown</surname><given-names>M. D.</given-names></name></person-group>. <year>1998</year>. <article-title>Effects of forage- and grain-based feeding systems on beef quality: A review</article-title>. <source>N. Z. J. Agric. Res.</source> <volume>41</volume>:<fpage>623</fpage>–<lpage>635</lpage>. <comment>doi:10.1080/00288233.1998.9513346</comment><uri>http://dx.doi.org/10.1080/00288233.1998.9513346</uri></citation></ref><ref id="r33"><citation citation-type="book"><person-group person-group-type="author"><collab>NAMP</collab></person-group>. <year>2011</year>. <article-title>The Meat Buyer’s Guide</article-title>. <publisher-name>North American Meat Processors Association</publisher-name>. <publisher-loc>Reston, VA 20191</publisher-loc>.</citation></ref><ref id="r34"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Neely</surname><given-names>T. R.</given-names></name><name><surname>Lorenzen</surname><given-names>C. L.</given-names></name><name><surname>Miller</surname><given-names>R. K.</given-names></name><name><surname>Tatum</surname><given-names>J. D.</given-names></name><name><surname>Wise</surname><given-names>J. W.</given-names></name><name><surname>Taylor</surname><given-names>J. F.</given-names></name><name><surname>Buyck</surname><given-names>M. J.</given-names></name><name><surname>Reagan</surname><given-names>J. O.</given-names></name><name><surname>Savell</surname><given-names>J. W.</given-names></name></person-group>. <year>1998</year>. <article-title>Beef customer satisfaction: Role of cut, USDA quality grade, and city on in-home consumer ratings</article-title>. <source>J. Anim. Sci.</source> <volume>76</volume>:<fpage>1027</fpage>–<lpage>1033</lpage>. <comment>doi:10.2527/1998.7641027x</comment><uri>http://dx.doi.org/10.2527/1998.7641027x</uri></citation></ref><ref id="r35"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Nuernberg</surname><given-names>K.</given-names></name><name><surname>Dannenberger</surname><given-names>D.</given-names></name><name><surname>Nuernberg</surname><given-names>G.</given-names></name><name><surname>Ender</surname><given-names>K.</given-names></name><name><surname>Voigt</surname><given-names>J.</given-names></name><name><surname>Scollan</surname><given-names>N. D.</given-names></name><name><surname>Wood</surname><given-names>J. D.</given-names></name><name><surname>Nute</surname><given-names>G. R.</given-names></name><name><surname>Richardson</surname><given-names>R. I.</given-names></name></person-group>. <year>2005</year>. <article-title>Effect of a grass-based and a concentrate feeding system on meat quality characteristics and fatty acid composition of longissimus muscle in different cattle breeds</article-title>. <source>Livest. Prod. Sci.</source> <volume>94</volume>:<fpage>137</fpage>–<lpage>147</lpage>. <comment>doi:10.1016/j.livprodsci.2004.11.036</comment><uri>http://dx.doi.org/10.1016/j.livprodsci.2004.11.036</uri></citation></ref><ref id="r36"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>O’Quinn</surname><given-names>T. G.</given-names></name><name><surname>Brooks</surname><given-names>J. C.</given-names></name><name><surname>Polkinghorne</surname><given-names>R. J.</given-names></name><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>Johnson</surname><given-names>B. J.</given-names></name><name><surname>Starkey</surname><given-names>J. D.</given-names></name><name><surname>Rathmann</surname><given-names>R. J.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name></person-group>. <year>2012</year>. <article-title>Consumer assessment of beef strip loin steaks of varying fat levels</article-title>. <source>J. Anim. Sci.</source> <volume>90</volume>:<fpage>626</fpage>–<lpage>634</lpage>. <comment>doi:10.2527/jas.2011-4282</comment><uri>http://dx.doi.org/10.2527/jas.2011-4282</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000300001000026&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r37"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Offer</surname><given-names>G.</given-names></name><name><surname>Trinick</surname><given-names>J.</given-names></name></person-group>. <year>1983</year>. <article-title>On the mechanism of water holding in meat: The swelling and shrinking of myofibrils</article-title>. <source>Meat Sci.</source> <volume>8</volume>:<fpage>245</fpage>–<lpage>281</lpage>. <comment>doi:10.1016/0309-1740(83)90013-X</comment><uri>http://dx.doi.org/10.1016/0309-1740(83)90013-X</uri></citation></ref><ref id="r38"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Papadopoulos</surname><given-names>L.</given-names></name><name><surname>Miller</surname><given-names>R.</given-names></name></person-group>. <year>1991</year>. <article-title>Consumer and trained sensory comparisons of cooked beef top rounds treated with sodium lactate</article-title>. <source>J. Food Sci.</source> <volume>56</volume>:<fpage>1141</fpage>–<lpage>1146</lpage>. <comment>doi:10.1111/j.1365-2621.1991.tb04720.x</comment><uri>http://dx.doi.org/10.1111/j.1365-2621.1991.tb04720.x</uri></citation></ref><ref id="r39"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Platter</surname><given-names>W. J.</given-names></name><name><surname>Tatum</surname><given-names>J. D.</given-names></name><name><surname>Belk</surname><given-names>K. E.</given-names></name><name><surname>Chapman</surname><given-names>P. L.</given-names></name><name><surname>Scanga</surname><given-names>J. A.</given-names></name><name><surname>Smith</surname><given-names>G. C.</given-names></name></person-group>. <year>2003</year>. <article-title>Relationships of consumer sensory ratings, marbling score, and shear force value to consumer acceptance of beef strip loin steaks</article-title>. <source>J. Anim. Sci.</source> <volume>81</volume>:<fpage>2741</fpage>–<lpage>2750</lpage>. <comment>doi:10.2527/2003.81112741x</comment><uri>http://dx.doi.org/10.2527/2003.81112741x</uri></citation></ref><ref id="r40"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Pringle</surname><given-names>T. D.</given-names></name><name><surname>Williams</surname><given-names>S. E.</given-names></name><name><surname>Lamb</surname><given-names>B. S.</given-names></name><name><surname>Johnson</surname><given-names>D. D.</given-names></name><name><surname>West</surname><given-names>R. L.</given-names></name></person-group>. <year>1997</year>. <article-title>Carcass characteristics, the calpain proteinase system, and aged tenderness of Angus and Brahman crossbred steers</article-title>. <source>J. Anim. Sci.</source> <volume>75</volume>:<fpage>2955</fpage>–<lpage>2961</lpage>. <comment>doi:10.2527/1997.75112955x</comment><uri>http://dx.doi.org/10.2527/1997.75112955x</uri></citation></ref><ref id="r41"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Reicks</surname><given-names>A. L.</given-names></name><name><surname>Brooks</surname><given-names>J. C.</given-names></name><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>Thompson</surname><given-names>L. D.</given-names></name><name><surname>Lyford</surname><given-names>C. L.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name></person-group>. <year>2011</year>. <article-title>Demographics and beef preferences affect consumer motivation for purchasing fresh beef steaks and roasts</article-title>. <source>Meat Sci.</source> <volume>87</volume>:<fpage>403</fpage>–<lpage>411</lpage>. <comment>doi:10.1016/j.meatsci.2010.11.018</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2010.11.018</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000287118900015&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r42"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Riley</surname><given-names>D. G.</given-names></name><name><surname>Johnson</surname><given-names>D. D.</given-names></name><name><surname>Chase</surname><given-names>C. C.</given-names></name><name><surname>West</surname><given-names>R. L.</given-names></name><name><surname>Coleman</surname><given-names>S. W.</given-names></name><name><surname>Olson</surname><given-names>T. A.</given-names></name><name><surname>Hammond</surname><given-names>A. C.</given-names></name></person-group>. <year>2005</year>. <article-title>Factors influencing tenderness in steaks from Brahman cattle</article-title>. <source>Meat Sci.</source> <volume>70</volume>:<fpage>347</fpage>–<lpage>356</lpage>. <comment>doi:10.1016/j.meatsci.2005.01.022</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2005.01.022</uri></citation></ref><ref id="r43"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Robbins</surname><given-names>K.</given-names></name><name><surname>Jensen</surname><given-names>J.</given-names></name><name><surname>Ryan</surname><given-names>K. J.</given-names></name><name><surname>Homco-Ryan</surname><given-names>C.</given-names></name><name><surname>McKeith</surname><given-names>F. K.</given-names></name><name><surname>Brewer</surname><given-names>M. S.</given-names></name></person-group>. <year>2003</year>. <article-title>Consumer attitudes towards beef and acceptability of enhanced beef</article-title>. <source>Meat Sci.</source> <volume>65</volume>:<fpage>721</fpage>–<lpage>729</lpage>. <comment>doi:10.1016/S0309-1740(02)00274-7</comment><uri>http://dx.doi.org/10.1016/S0309-1740(02)00274-7</uri></citation></ref><ref id="r44"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sadkowski</surname><given-names>T.</given-names></name><name><surname>Ciecierska</surname><given-names>A.</given-names></name><name><surname>Majewska</surname><given-names>A.</given-names></name><name><surname>Oprządek</surname><given-names>J.</given-names></name><name><surname>Dasiewicz</surname><given-names>K.</given-names></name><name><surname>Ollik</surname><given-names>M.</given-names></name><name><surname>Wicik</surname><given-names>Z.</given-names></name><name><surname>Motyl</surname><given-names>T.</given-names></name></person-group>. <year>2014</year>. <article-title>Transcriptional background of beef marbling- novel genes implicated in intramuscular fat deposition</article-title>. <source>Meat Sci.</source> <volume>97</volume>:<fpage>32</fpage>–<lpage>41</lpage>. <comment>doi:10.1016/j.meatsci.2013.12.017</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2013.12.017</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000333784900005&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r45"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Savell</surname><given-names>J. W.</given-names></name><name><surname>Branson</surname><given-names>R. E.</given-names></name><name><surname>Cross</surname><given-names>H. R.</given-names></name><name><surname>Stiffler</surname><given-names>D. M.</given-names></name><name><surname>Wise</surname><given-names>J. W.</given-names></name><name><surname>Griffin</surname><given-names>D. B.</given-names></name><name><surname>Smith</surname><given-names>G. C.</given-names></name></person-group>. <year>1987</year>. <article-title>National Consumer Retail Beef Study: Palatability Evaluations of Beef Loin Steaks that Differed in Marbling</article-title>. <source>J. Food Sci</source>. <volume>52</volume>:<fpage>517</fpage>–<lpage>519</lpage>. <comment>doi:10.1111/j.1365-2621.1987.tb06664.x</comment><uri>http://dx.doi.org/10.1111/j.1365-2621.1987.tb06664.x</uri></citation></ref><ref id="r46"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Scanga</surname><given-names>J. A.</given-names></name><name><surname>Delmore</surname><given-names>R. J.</given-names><suffix>Jr</suffix></name><name><surname>Ames</surname><given-names>R. P.</given-names></name><name><surname>Belk</surname><given-names>K. E.</given-names></name><name><surname>Tatum</surname><given-names>J. D.</given-names></name><name><surname>Smith</surname><given-names>G. C.</given-names></name></person-group>. <year>2000</year>. <article-title>Palatability of beef steaks marinated with solutions of calcium chloride, phosphate, and (or) beef-flavoring</article-title>. <source>Meat Sci.</source> <volume>55</volume>:<fpage>397</fpage>–<lpage>401</lpage>. <comment>doi:10.1016/S0309-1740(99)00168-0</comment><uri>http://dx.doi.org/10.1016/S0309-1740(99)00168-0</uri></citation></ref><ref id="r47"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sheard</surname><given-names>P. R.</given-names></name><name><surname>Tali</surname><given-names>A.</given-names></name></person-group>. <year>2004</year>. <article-title>Injection of salt, tripolyphosphate and bicarbonate marinade solutions to improve the yield and tenderness of cooked pork loin</article-title>. <source>Meat Sci.</source> <volume>68</volume>:<fpage>305</fpage>–<lpage>311</lpage>. <comment>doi:10.1016/j.meatsci.2004.03.012</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2004.03.012</uri></citation></ref><ref id="r48"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Smith</surname><given-names>A. M.</given-names></name><name><surname>Harris</surname><given-names>K. B.</given-names></name><name><surname>Haneklaus</surname><given-names>A. N.</given-names></name><name><surname>Savell</surname><given-names>J. W.</given-names></name></person-group>. <year>2011</year>. <article-title>Proximate composition and energy content of beef steaks as influenced by USDA quality grade and degree of doneness</article-title>. <source>Meat Sci.</source> <volume>89</volume>:<fpage>228</fpage>–<lpage>232</lpage>. <comment>doi:10.1016/j.meatsci.2011.04.027</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2011.04.027</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000292677900017&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r49"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Smith</surname><given-names>G. C.</given-names></name><name><surname>Carpenter</surname><given-names>Z. L.</given-names></name><name><surname>Cross</surname><given-names>H. R.</given-names></name><name><surname>Murphey</surname><given-names>C. E.</given-names></name><name><surname>Abraham</surname><given-names>H. C.</given-names></name><name><surname>Savell</surname><given-names>J. W.</given-names></name><name><surname>Davis</surname><given-names>G. W.</given-names></name><name><surname>Berry</surname><given-names>B. W.</given-names></name><name><surname>Parrish</surname><given-names>F. C.</given-names></name></person-group>. <year>1985</year>. <article-title>Relationship of USDA marbling groups to palatability of cooked beef</article-title>. <source>J. Food Qual</source>. <volume>7</volume>:<fpage>289</fpage>–<lpage>308</lpage>. <comment>doi:10.1111/j.1745-4557.1985.tb01061.x</comment><uri>http://dx.doi.org/10.1111/j.1745-4557.1985.tb01061.x</uri></citation></ref><ref id="r50"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Stetzer</surname><given-names>A. J.</given-names></name><name><surname>Cadwallader</surname><given-names>K.</given-names></name><name><surname>Singh</surname><given-names>T. K.</given-names></name><name><surname>McKeith</surname><given-names>F. K.</given-names></name><name><surname>Brewer</surname><given-names>M. S.</given-names></name></person-group>. <year>2008</year>. <article-title>Effect of enhancement and ageing on flavor and volatile compounds in various beef muscles</article-title>. <source>Meat Sci.</source> <volume>79</volume>:<fpage>13</fpage>–<lpage>19</lpage>. <comment>doi:10.1016/j.meatsci.2007.07.025</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2007.07.025</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000254730100002&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r51"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Tedford</surname><given-names>J. L.</given-names></name><name><surname>Rodas-González</surname><given-names>A.</given-names></name><name><surname>Garmyn</surname><given-names>A. J.</given-names></name><name><surname>Brooks</surname><given-names>J. C.</given-names></name><name><surname>Johnson</surname><given-names>B. J.</given-names></name><name><surname>Starkey</surname><given-names>J. D.</given-names></name><name><surname>Clark</surname><given-names>G. O.</given-names></name><name><surname>Derington</surname><given-names>A. J.</given-names></name><name><surname>Collins</surname><given-names>J. A.</given-names></name><name><surname>Miller</surname><given-names>M. F.</given-names></name></person-group>. <year>2014</year>. <article-title>U. S. consumer perceptions of U. S. and Canadian beef quality grades</article-title>. <source>J. Anim. Sci.</source> <volume>92</volume>:<fpage>3685</fpage>–<lpage>3692</lpage>. <comment>doi:10.2527/jas.2014-7739</comment><uri>http://dx.doi.org/10.2527/jas.2014-7739</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000341663600047&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r52"><citation citation-type="book"><person-group person-group-type="author"><name><surname>Umberger</surname><given-names>W.</given-names></name><name><surname>Feuz</surname><given-names>D.</given-names></name><name><surname>Calkins</surname><given-names>C.</given-names></name><name><surname>Killinger</surname><given-names>K.</given-names></name></person-group>. <year>2000</year>. <article-title>The Value of Beef Flavor: Consumer Willingness-to-Pay for Marbling in Beef Steaks</article-title>. <publisher-name>Annual Meeting of the Western Agricultural Economics Association</publisher-name>. <publisher-loc>Vancouver, British Columbia</publisher-loc>. <comment>29 June – 1 July, 2000</comment></citation></ref><ref id="r53"><citation citation-type="journal"><person-group person-group-type="author"><collab>USDL</collab></person-group>. <year>2017</year>. <article-title>History of federal minimum wage rates under the Fair Labor Standards Act, 1938-2009</article-title>. <source>United States Department of Labor</source>. <comment><ext-link ext-link-type="uri" xlink:href="https://www.dol.gov/whd/minwage/chart.htm" xmlns:xlink="http://www.w3.org/1999/xlink">https://www.dol.gov/whd/minwage/chart.htm</ext-link> (accessed 2 August 2017)</comment>.</citation></ref><ref id="r54"><citation citation-type="web"><person-group person-group-type="author"><collab>USDA</collab></person-group>. <year>2012</year>. <article-title>Cattle and Beef. Economic Research Service</article-title>. <comment><ext-link ext-link-type="uri" xlink:href="http://www.fas.usda.gov/data/central-america-and-caribbean-prospects-us-%20agricultural-exports" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.fas.usda.gov/data/central-america-and-caribbean-prospects-us- agricultural-exports</ext-link> (Accessed 18 September 2015.)</comment></citation></ref><ref id="r55"><citation citation-type="journal"><person-group person-group-type="author"><collab>USDA</collab></person-group>. <year>2015</year>. <article-title>Livestock and meat international trade data</article-title>. <source>Economic Research Service</source>. <comment><ext-link ext-link-type="uri" xlink:href="http://www.fas.usda.gov/data/central-america-and-caribbean-prospects-us-agricultural-exports" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.fas.usda.gov/data/central-america-and-caribbean-prospects-us-agricultural-exports</ext-link> (Accessed 18 September 2015.)</comment></citation></ref><ref id="r56"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Van Elswyk</surname><given-names>M. E.</given-names></name><name><surname>McNeill</surname><given-names>S. H.</given-names></name></person-group>. <year>2014</year>. <article-title>Impact of grass/forage feeding versus grain finishing on beef nutrients and sensory quality: The U.S. experience</article-title>. <source>Meat Sci.</source> <volume>96</volume>:<fpage>535</fpage>–<lpage>540</lpage>. <comment>doi:10.1016/j.meatsci.2013.08.010</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2013.08.010</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000327166500066&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r57"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Vote</surname><given-names>D. J.</given-names></name><name><surname>Platter</surname><given-names>W. J.</given-names></name><name><surname>Tatum</surname><given-names>J. D.</given-names></name><name><surname>Schmidt</surname><given-names>G. R.</given-names></name><name><surname>Belk</surname><given-names>K. E.</given-names></name><name><surname>Smith</surname><given-names>G. C.</given-names></name><name><surname>Speer</surname><given-names>N. C.</given-names></name></person-group>. <year>2000</year>. <article-title>Injection of beef strip loins with solutions containing sodium tripolyphosphate, sodium lactate, and sodium chloride to enhance palatability</article-title>. <source>J. Anim. Sci.</source> <volume>78</volume>:<fpage>952</fpage>–<lpage>957</lpage>. <comment>doi:10.2527/2000.784952x</comment><uri>http://dx.doi.org/10.2527/2000.784952x</uri></citation></ref><ref id="r58"><citation citation-type="journal"><person-group person-group-type="author"><collab>WageIndicator</collab></person-group>. <year>2015</year>. <article-title>Minimum wages in Honduras with effect from 01-01-2015 to 31-12-2015</article-title>. <source>WageIndicator Foundation</source>. <comment><ext-link ext-link-type="uri" xlink:href="http://www.wageindicator.org/main/salary/minimum-wage/honduras/archive/1" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.wageindicator.org/main/salary/minimum-wage/honduras/archive/1</ext-link> (accessed 8 August 2017)</comment>.</citation></ref><ref id="r59"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Warren</surname><given-names>H. E.</given-names></name><name><surname>Scollan</surname><given-names>N. D.</given-names></name><name><surname>Nute</surname><given-names>G. R.</given-names></name><name><surname>Hughes</surname><given-names>S. I.</given-names></name><name><surname>Wood</surname><given-names>J. D.</given-names></name><name><surname>Richardson</surname><given-names>R. I.</given-names></name></person-group>. <year>2008</year>. <article-title>Effects of breed and a concentrate or grass silage diet on beef quality in cattle of 3 ages. II: Meat stability and flavour</article-title>. <source>Meat Sci.</source> <volume>78</volume>:<fpage>270</fpage>–<lpage>278</lpage>. <comment>doi:10.1016/j.meatsci.2007.06.007</comment><uri>http://dx.doi.org/10.1016/j.meatsci.2007.06.007</uri><ext-link ext-link-type="WOS">http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;SrcApp=PARTNER_APP&amp;SrcAuth=Agronomy_sub&amp;KeyUT=WOS:000253028000015&amp;DestLinkType=FullRecord&amp;DestApp=WOS_CPL&amp;UsrCustomerID=9992b2403adf8c36119d0b6fce39b97c</ext-link></citation></ref><ref id="r60"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Whipple</surname><given-names>G.</given-names></name><name><surname>Koohmaraie</surname><given-names>M.</given-names></name><name><surname>Dikeman</surname><given-names>M. E.</given-names></name><name><surname>Crouse</surname><given-names>J. D.</given-names></name><name><surname>Hunt</surname><given-names>M. C.</given-names></name><name><surname>Klemm</surname><given-names>R. D.</given-names></name></person-group>. <year>1990</year>. <article-title>Evaluation of attributes that affect longissimus muscle tenderness in <italic>Bos taurus</italic> and <italic>Bos indicus</italic> cattle</article-title>. <source>J. Anim. Sci.</source> <volume>68</volume>:<fpage>2716</fpage>–<lpage>2728</lpage>. <comment>doi:10.2527/1990.6892716x</comment><uri>http://dx.doi.org/10.2527/1990.6892716x</uri></citation></ref><ref id="r61"><citation citation-type="journal"><person-group person-group-type="author"><name><surname>Wicklund</surname><given-names>S. E.</given-names></name><name><surname>Homco-Ryan</surname><given-names>C.</given-names></name><name><surname>Ryan</surname><given-names>K. J.</given-names></name><name><surname>McKeith</surname><given-names>F. K.</given-names></name><name><surname>McFarlane</surname><given-names>B. J.</given-names></name><name><surname>Brewer</surname><given-names>M. S.</given-names></name></person-group>. <year>2006</year>. <article-title>Aging and Enhancement Effects on Quality Characteristics of Beef Strip Steaks</article-title>. <source>J. Food Sci</source>. <volume>70</volume>:<fpage>S242</fpage>–<lpage>S248</lpage>. <comment>doi:10.1111/j.1365-2621.2005.tb07164.x</comment><uri>http://dx.doi.org/10.1111/j.1365-2621.2005.tb07164.x</uri></citation></ref></ref-list></back><custom-meta-container><journal-date-data><jdate>2018-08-16</jdate></journal-date-data><journal-year>2018</journal-year><journal-month>08</journal-month><journal-title>Meat and Muscle Biology</journal-title><journal-issue>1</journal-issue><journal-fpage>242</journal-fpage><journal-volume>2</journal-volume><journal-lpage>253</journal-lpage><insert-date>August 16, 2018</insert-date></custom-meta-container></article>