Health

Diagnostic Performance of a RT-PCR Test for the Detection of PRRS Virus in Serum

Authors
  • Elizabeth A. Wagstrom (Iowa State University)
  • Kyoung-Jin Yoon (Iowa State University)
  • Jeffrey J. Zimmerman (Iowa State University)

Abstract

Polymerase chain reaction (PCR)-based testing is being widely implemented for diagnostic purposes. The PCR technique is generally considered to be both analytically sensitive (i.e., detects small quantities of a substance) and analytically specific (i.e., few cross reactions). There are few studies, however, of diagnostic sensitivity and specificity (i.e., the likelihood of correctly identifying infection status). The purpose of the following study was to estimate the diagnostic sensitivity and specificity, as well as the analytical sensitivity, of a RT-PCR currently being used in a midwestern diagnostic laboratory for the detection of PRRSV RNA.

Keywords: ASL R1602

How to Cite:

Wagstrom, E. A., Yoon, K. & Zimmerman, J. J., (1999) “Diagnostic Performance of a RT-PCR Test for the Detection of PRRS Virus in Serum”, Iowa State University Animal Industry Report 1(1).

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Published on
01 Jan 1999
Peer Reviewed