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Steady-State and Time-Resolved Spectroscopy of F420 Extracted from Methanogen Cells

Authors: , , , , ,

Abstract

The methanogen electron carrier F420 was extracted from a methanogen cell culture, Methanobrevibacter ruminantium, and separated from other cell components by a single anion exchange chromatographic process. The extent of separation was determined spectroscopically. The fluorescence lifetime of F420 at pH 7.5 is ~4.2 nanoseconds.

Keywords: Chemistry, ASL R1611, Veterinary Microbiology and Preventive Medicine

How to Cite: Ashby, K. D. , Kraus, G. A. , Petrich, J. W. , Casey, T. A. , Rasmussen, M. A. & Carpenter, S. L. (1999) “Steady-State and Time-Resolved Spectroscopy of F420 Extracted from Methanogen Cells”, Iowa State University Animal Industry Report. 1(1).