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Steady-State and Time-Resolved Spectroscopy of F420 Extracted from Methanogen Cells

Authors
  • K. D. Ashby (Iowa State University)
  • George A. Kraus (Iowa State University)
  • J. W. Petrich (Iowa State University)
  • T. A. Casey (United States Department of Agriculture)
  • M. A. Rasmussen (United States Department of Agriculture)
  • Susan L. Carpenter (Iowa State University)

Abstract

The methanogen electron carrier F420 was extracted from a methanogen cell culture, Methanobrevibacter ruminantium, and separated from other cell components by a single anion exchange chromatographic process. The extent of separation was determined spectroscopically. The fluorescence lifetime of F420 at pH 7.5 is ~4.2 nanoseconds.

Keywords: Chemistry, ASL R1611, Veterinary Microbiology and Preventive Medicine

How to Cite:

Ashby, K. D., Kraus, G. A., Petrich, J. W., Casey, T. A., Rasmussen, M. A. & Carpenter, S. L., (1999) “Steady-State and Time-Resolved Spectroscopy of F420 Extracted from Methanogen Cells”, Iowa State University Animal Industry Report 1(1).

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Published on
01 Jan 1999
Peer Reviewed
License
Creative Commons Attribution-NonCommercial 4.0