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Triplex PCR for Rapid Detection of Escherichia coli O157:H7 Directly from Ground Pork

Authors
  • I. Matise (Iowa State University)
  • M. Shelton (Iowa State University)
  • G. Phillips (Iowa State University)
  • L. A. Will (Iowa State University)

Abstract

A polymerase chain reaction (PCR) procedure is being developed to detect Escherichia coli O157:H7 and associated Shiga-like toxins (STX1 and STX2) directly from pork, bypassing the time-consuming microbial culture steps for identification. A triplex PCR procedure was adapted and conditions experimentally determined to efficiently amplify three target genes: uidA with unique sequence in E. coli O157:H7, and stx1 and stx2. A meat sample preparation technique developed in previous research was modified to allow detection of 0.2-2.0 colony forming units per gram (CFU./g.) of meat. Time required to complete the procedure after overnight incubation of samples is seven hours.

Keywords: ASL R1420

How to Cite:

Matise, I., Shelton, M., Phillips, G. & Will, L. A., (1997) “Triplex PCR for Rapid Detection of Escherichia coli O157:H7 Directly from Ground Pork”, Iowa State University Animal Industry Report 1(1).

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Published on
01 Jan 1997
Peer Reviewed