Health

Application of Immunohistochemistry and ELISA for the Diagnosis of Neospora-Infected Cattle

Authors
  • Meghan A. Hessler (Iowa State University)
  • Patrick Halbur (Iowa State University)

Abstract

Studies were undertaken to adapt diagnostic methods for use in our laboratory for detection of Neospora sp. infection in cattle. An immunohistochemical (IHC) test was used for detection of Neospora sp. antigen in tissues of aborted bovine fetuses. Neospora sp. antigen was detected most frequently in fetal brain tissue. Polyclonal antibodies were tested for specificity and sensitivity of the IHC. Sera were obtained from Neospora sp. infected dairy herds for use as positive and negative controls in the continuing development of an enzyme-linked immunosorbent assay (ELISA).

Keywords: ASL R1358

How to Cite:

Hessler, M. A. & Halbur, P., (1997) “Application of Immunohistochemistry and ELISA for the Diagnosis of Neospora-Infected Cattle”, Iowa State University Animal Industry Report 1(1).

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Published on
01 Jan 1997
Peer Reviewed